FastQCFastQC Report
Thu 20 Oct 2022
DF3-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDF3-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11257350
Sequences flagged as poor quality0
Sequence length100
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGGCGGTCGAGTGCCTCACAGTGTATCAAGGGT426660.37900571626537327No Hit
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT360050.31983548526074074No Hit
CGACATGGCTACGATGGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCA213210.18939626110940852No Hit
CGACATGGCTACGATGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCAA213030.18923636557449133No Hit
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG160050.14217377979719917No Hit
CGACATGGCTACGATGGGAGTACTCAAGTCATCGTTCTCTAGTTCAGAGT129660.11517808365201401No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA118960.10567318240971453No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGC3215800.090.593163
CATGGCT3225500.090.529094
CGACATG3228800.089.962021
GACATGG3257750.089.417942
ATGGCTA3284300.088.8439255
GCTACGA3335300.086.404588
TGGCTAC3392750.086.026186
GGCTACG3398250.085.765237
CTACGAT3331400.085.292439
ACGATGG3197900.042.79588310-11
TACGATG3245200.042.7912610-11
GATGGGA1074050.038.55658312-13
CGATGGG3513950.038.38900812-13
GATGGGG1955050.038.14835712-13
ATGGGGG395000.038.03430614-15
ATGGGGA894250.037.06916414-15
TGGGGAA280900.036.9357414-15
ATGGGGC290300.035.82879614-15
ATGGGAA400350.035.19570514-15
TGGGGGA137200.035.130114-15