FastQCFastQC Report
Thu 20 Oct 2022
DF2-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDF2-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5652044
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT599661.0609613088645453No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA269490.47680095908665965No Hit
CGACATGGCTACGATGGGGGCGGTCGAGTGCCTCACAGTGTATCAAGGGT192800.3411155327170135No Hit
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA124820.2208404605484317No Hit
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG123860.2191419599705876No Hit
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA100150.17719253424071008No Hit
CGACATGGCTACGATGGGGACGATGAGGACGAAGAGGAGTATGACGAGAA75390.1333853735038156No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG1444450.090.375241
ACATGGC1454250.090.066773
CATGGCT1458800.090.027484
GACATGG1459800.089.71792
ATGGCTA1485100.088.350885
GCTACGA1499100.086.346958
TGGCTAC1521350.086.2827766
GGCTACG1525750.085.913817
CTACGAT1485000.085.236169
TACGATG1422050.042.72487310-11
ACGATGG1388000.042.69956610-11
GATGGGA400000.039.3742512-13
GATGGGG899200.039.15708512-13
CGATGGG1487950.039.15692512-13
ATGGGGG165550.038.58230214-15
ATGGGGA395000.038.44481314-15
TGGGGAA146300.036.3021214-15
ATGGGGC105500.036.2857814-15
ATGGGAA167800.036.2163314-15
ATGGGGT269150.036.12966514-15
Produced by FastQC (version 0.11.5)