Basic Statistics
Measure | Value |
---|---|
Filename | DF2-MGI-1_parsed.fq.gz |
File type | Conventional base calls |
Encoding | Sanger / Illumina 1.9 |
Total Sequences | 5256442 |
Sequences flagged as poor quality | 0 |
Sequence length | 100 |
%GC | 43 |
Per base sequence quality
Per sequence quality scores
Per base sequence content
Per sequence GC content
Per base N content
Sequence Length Distribution
Sequence Duplication Levels
Overrepresented sequences
Sequence | Count | Percentage | Possible Source |
---|---|---|---|
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT | 50133 | 0.9537439964143046 | No Hit |
CGACATGGCTACGATGGGGGCGGTCGAGTGCCTCACAGTGTATCAAGGGT | 24197 | 0.46033039078524973 | No Hit |
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 20023 | 0.38092306544997545 | No Hit |
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG | 14184 | 0.2698403216472283 | No Hit |
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA | 10452 | 0.19884172601923505 | No Hit |
CGACATGGCTACGATGGGGACGATGAGGACGAAGAGGAGTATGACGAGAA | 8908 | 0.1694682448698188 | No Hit |
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA | 7344 | 0.13971427821328572 | No Hit |
CGACATGGCTACGATGGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCA | 6256 | 0.11901586662613228 | No Hit |
CGACATGGCTACGATGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCAA | 5603 | 0.10659301481876905 | No Hit |
Adapter Content
Kmer Content
Sequence | Count | PValue | Obs/Exp Max | Max Obs/Exp Position |
---|---|---|---|---|
CGACATG | 159760 | 0.0 | 90.7433 | 1 |
ACATGGC | 160545 | 0.0 | 90.62456 | 3 |
CATGGCT | 161040 | 0.0 | 90.617424 | 4 |
GACATGG | 161670 | 0.0 | 89.99103 | 2 |
ATGGCTA | 164565 | 0.0 | 88.61071 | 5 |
TGGCTAC | 167980 | 0.0 | 86.83164 | 6 |
GCTACGA | 166095 | 0.0 | 86.53806 | 8 |
GGCTACG | 168430 | 0.0 | 86.44618 | 7 |
CTACGAT | 164010 | 0.0 | 85.781235 | 9 |
ACGATGG | 152990 | 0.0 | 42.990913 | 10-11 |
TACGATG | 156875 | 0.0 | 42.98983 | 10-11 |
GATGGGA | 44130 | 0.0 | 39.454227 | 12-13 |
GATGGGG | 99030 | 0.0 | 39.40634 | 12-13 |
CGATGGG | 164660 | 0.0 | 39.276085 | 12-13 |
ATGGGGG | 19125 | 0.0 | 38.816475 | 14-15 |
ATGGGGA | 44045 | 0.0 | 38.714043 | 14-15 |
CTACGAA | 2795 | 0.0 | 38.33989 | 9 |
TGGGGAA | 15225 | 0.0 | 37.80066 | 14-15 |
ATGGGGT | 26990 | 0.0 | 36.77807 | 14-15 |
ATGGGGC | 12170 | 0.0 | 36.727196 | 14-15 |