FastQCFastQC Report
Thu 20 Oct 2022
DF2-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDF2-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5256442
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT501330.9537439964143046No Hit
CGACATGGCTACGATGGGGGCGGTCGAGTGCCTCACAGTGTATCAAGGGT241970.46033039078524973No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA200230.38092306544997545No Hit
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG141840.2698403216472283No Hit
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA104520.19884172601923505No Hit
CGACATGGCTACGATGGGGACGATGAGGACGAAGAGGAGTATGACGAGAA89080.1694682448698188No Hit
CGACATGGCTACGATGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA73440.13971427821328572No Hit
CGACATGGCTACGATGGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCA62560.11901586662613228No Hit
CGACATGGCTACGATGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCAA56030.10659301481876905No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG1597600.090.74331
ACATGGC1605450.090.624563
CATGGCT1610400.090.6174244
GACATGG1616700.089.991032
ATGGCTA1645650.088.610715
TGGCTAC1679800.086.831646
GCTACGA1660950.086.538068
GGCTACG1684300.086.446187
CTACGAT1640100.085.7812359
ACGATGG1529900.042.99091310-11
TACGATG1568750.042.9898310-11
GATGGGA441300.039.45422712-13
GATGGGG990300.039.4063412-13
CGATGGG1646600.039.27608512-13
ATGGGGG191250.038.81647514-15
ATGGGGA440450.038.71404314-15
CTACGAA27950.038.339899
TGGGGAA152250.037.8006614-15
ATGGGGT269900.036.7780714-15
ATGGGGC121700.036.72719614-15