FastQCFastQC Report
Thu 20 Oct 2022
DF1-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDF1-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9646518
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGGCGGTCGAGTGCCTCACAGTGTATCAAGGGT389820.40410436180184395No Hit
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT359910.37309835528218577No Hit
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG161790.16771854880693737No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA153230.15884488060873364No Hit
CGACATGGCTACGATGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCAA148870.15432511503114388No Hit
CGACATGGCTACGATGGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCA132610.13746929202848115No Hit
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA124500.12906211339677176No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGC2886500.090.570873
CATGGCT2894850.090.506074
CGACATG2893700.090.148981
GACATGG2920250.089.5128562
ATGGCTA2961300.088.392635
TGGCTAC3032850.086.32596
GCTACGA3004550.086.291168
GGCTACG3044000.085.947937
CTACGAT2997100.085.169569
TACGATG2916250.042.6654410-11
ACGATGG2872650.042.54566210-11
GATGGGA987100.037.42716212-13
CGATGGG3232900.037.19337512-13
ATGGGGG372550.037.1344814-15
GATGGGG1769900.037.12944412-13
ATGGGGA784550.036.39347514-15
TGGGGAA256300.035.65801614-15
ATGGGGC237250.034.84636714-15
TGGGGAG220300.034.78597314-15
TGGGGGA131500.034.6334614-15