FastQCFastQC Report
Thu 20 Oct 2022
DF1-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDF1-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9614295
Sequences flagged as poor quality0
Sequence length100
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT484050.5034690531130988No Hit
CGACATGGCTACGATGGGGGCGGTCGAGTGCCTCACAGTGTATCAAGGGT413800.4304007730155981No Hit
CGACATGGCTACGATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA197810.20574571510443562No Hit
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG163180.16972643339943283No Hit
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA153180.1593252547378669No Hit
CGACATGGCTACGATGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCAA145840.15169078960027751No Hit
CGACATGGCTACGATGGGGAGTTCTGGATCAAGCAGAGTAAAACTATCCA132520.13783641962307167No Hit
CGACATGGCTACGATGGGGCGGTCGAGTGCCTCACAGTGTATCAAGGGTT100930.10497909623118493No Hit
CGACATGGCTACGATGGGGACGATGAGGACGAAGAGGAGTATGACGAGAA96590.1004649846920653No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGC3071700.090.698053
CATGGCT3085450.090.517794
CGACATG3076200.090.220081
GACATGG3101900.089.808962
ATGGCTA3144500.088.7551965
TGGCTAC3213350.086.869596
GCTACGA3186750.086.75558
GGCTACG3219550.086.62937
CTACGAT3182500.085.6086659
TACGATG3104500.042.87983710-11
ACGATGG3056400.042.84344510-11
GATGGGA1052100.037.9493412-13
CGATGGG3406900.037.82252512-13
GATGGGG1863300.037.76294312-13
ATGGGGG392650.037.280414-15
ATGGGGA804300.037.14186514-15
TGGGGAA263800.036.8356714-15
ATGGGGC250300.035.82740414-15
TGGGGAG221050.035.0825614-15
ATGGGAA401750.035.0087114-15