FastQCFastQC Report
Thu 20 Oct 2022
D3-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33208954
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAG698260.21026256954675537No Hit
CTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATG696460.2097205470548696No Hit
CTCGATGTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAG584280.17594050086612184No Hit
GTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTT556270.167506028645166No Hit
GCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATGATC455300.13710157808644016No Hit
GTAGTTTCTTTAGTACGAAAGGATTAGGAAATTGAGACAAATGATCTCTT452510.13626144322401723No Hit
CTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCT439510.13234683633817554No Hit
CTCTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATC380300.11451730759119966No Hit
GGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAGTTTCTTTAG378240.11389699296159704No Hit
GATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGAC375810.11316526259755125No Hit
CTAGGGATAACAGCGTAATCTTTTTGGAGAGTTCAAATCGATAAAAAGGT366260.11028953215449061No Hit
CTGTTCGACTTTTAATCTCTTACATGATCTGAGTTCAAACCGGCGTAAGC343700.10349618358952227No Hit
ATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTA340930.10266207119923139No Hit
GGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATG332900.10024404863820764No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG424250.063.113491
ACATGGC438550.061.248433
CATGGCT477700.056.4846084
GGCTACG505150.053.052367
GCTACGA512750.051.8626988
ATGGCTA520950.051.7230035
CTACGAT573750.046.0948149
TGGCTAC604500.044.745246
GACATGG667850.040.247512
GGCCTCC88100.035.1032948
CGCTTGG96550.033.880893
CCGCTTG104450.031.2733352
GCCTCCG99750.030.767929
TGGCCTC100350.030.2092677
ACCGCTT121950.026.7470281
TACGATG560950.023.44344110-11
ACGATGG566950.022.8181510-11
GCTTGGC151900.021.8446354
CGATGGG587000.021.06192612-13
CCTCCGA87200.018.56823310-11
Produced by FastQC (version 0.11.5)