FastQCFastQC Report
Thu 20 Oct 2022
D3-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD3-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29774468
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAG584700.1963763046916573No Hit
CTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATG577310.1938943124021561No Hit
CTCGATGTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAG459090.1541891529346553No Hit
GTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTT434200.14582964169166682No Hit
GCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATGATC373710.12551357760615572No Hit
CTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCT360620.12111719342894724No Hit
GTAGTTTCTTTAGTACGAAAGGATTAGGAAATTGAGACAAATGATCTCTT356050.11958232133652227No Hit
GGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAGTTTCTTTAG313290.10522102359645856No Hit
CTCTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATC310530.10429405489293714No Hit
GATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGAC304050.10211769358901727No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG486650.067.643691
ACATGGC497900.066.33243
CATGGCT541100.061.2276884
GGCTACG589700.055.8389057
ATGGCTA599050.055.3361175
GCTACGA591750.055.240398
CTACGAT629400.051.428199
TGGCTAC682300.048.618796
GACATGG765350.043.1588172
GGCCTCC70850.035.623158
CGCTTGG81950.032.4039043
CCGCTTG91250.028.9468482
TGGCCTC86000.028.9104677
GCCTCCG90350.027.622589
TACGATG638900.025.03380810-11
ACGATGG637750.024.69572810-11
ACCGCTT107900.024.3929581
CGATGGG662200.022.83637812-13
GCTTGGC122200.021.9615384
GATGGGA374800.019.56870512-13