FastQCFastQC Report
Thu 20 Oct 2022
D2-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD2-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10863108
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAG208170.191630240627268No Hit
CTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATG207500.1910134742285541No Hit
CTCGATGTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAG189680.17460932911649227No Hit
GTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTT184860.1701722932332073No Hit
GCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATGATC154100.14185627170419368No Hit
CTAGGGATAACAGCGTAATCTTTTTGGAGAGTTCAAATCGATAAAAAGGT131760.12129125476797248No Hit
GATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGAC130930.12052720087105827No Hit
CTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCT128770.1185388196453538No Hit
CTCTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATC118750.10931494007055807No Hit
GTAGTTTCTTTAGTACGAAAGGATTAGGAAATTGAGACAAATGATCTCTT118690.10925970725873295No Hit
GGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAGTTTCTTTAG114190.10511724637184865No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG169350.065.275471
ACATGGC177700.062.3140143
CATGGCT191750.058.0177354
ATGGCTA208200.053.388575
GGCTACG208850.053.0648777
GCTACGA212650.051.8292968
CTACGAT223750.048.922019
TGGCTAC243600.045.9388356
GACATGG246750.044.876192
GGCCTCC25350.039.6765338
CGCTTGG31600.033.7626573
GCCTCCG30600.033.6372579
TGGCCTC32800.030.5213437
CCGCTTG35750.029.8433552
ACCGCTT40250.026.506831
GCTTGGC43900.024.0888394
TACGATG229950.023.60730610-11
ACGATGG225000.023.47911310-11
CGATGGG232150.022.04738212-13
GATGGGA123100.018.44110512-13