FastQCFastQC Report
Thu 20 Oct 2022
D1-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD1-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13189128
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAG285810.2167012102695493No Hit
CTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATG282580.2142522234980205No Hit
CTCGATGTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAG243000.184242658043807No Hit
GTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTT223050.1691165632784821No Hit
GTAGTTTCTTTAGTACGAAAGGATTAGGAAATTGAGACAAATGATCTCTT196480.14897118293188147No Hit
GCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATGATC192580.1460142020003142No Hit
CTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCT187560.1422080368012199No Hit
GGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAGTTTCTTTAG163720.12413254310671638No Hit
CTCTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATC159420.12087228207960374No Hit
GATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGAC152320.11548906038367358No Hit
CTGTTCGACTTTTAATCTCTTACATGATCTGAGTTCAAACCGGCGTAAGC150200.11388167587728316No Hit
CTAGGGATAACAGCGTAATCTTTTTGGAGAGTTCAAATCGATAAAAAGGT142140.10777058195204413No Hit
ATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTA136800.10372179267651356No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG158750.064.926611
ACATGGC166050.062.157183
CATGGCT180350.057.5414474
GCTACGA186250.054.9616138
GGCTACG189650.054.5214847
ATGGCTA196150.052.9783335
CTACGAT201500.049.868989
TGGCTAC242500.043.0268026
GACATGG244800.042.180962
GGCCTCC31150.035.910118
CGCTTGG38300.031.2924273
TGGCCTC36900.030.4417347
GCCTCCG36750.029.6707489
ACCGCTT44550.026.3748591
CCGCTTG44700.025.9709172
ACGATGG207000.023.6248810-11
TACGATG213250.023.50551210-11
GCTTGGC52600.023.1425864
CGATGGG207150.022.7342512-13
GATGGGA120950.018.4774712-13
Produced by FastQC (version 0.11.5)