FastQCFastQC Report
Thu 20 Oct 2022
D1-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameD1-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13684767
Sequences flagged as poor quality0
Sequence length100
%GC37

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAG296170.21642312214742132No Hit
CTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATG294070.21488856916599314No Hit
CTCGATGTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAG259050.18929807135189075No Hit
GTTGGATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTT247000.18049266019655286No Hit
GCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATGATC215820.15770820211991918No Hit
GTAGTTTCTTTAGTACGAAAGGATTAGGAAATTGAGACAAATGATCTCTT200410.1464475061942962No Hit
CTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCT192400.14059428267942012No Hit
GATTAAGAGATTGGCAAGGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGAC170710.1247445425998119No Hit
CTCTTACATGATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATC170000.12422571754418617No Hit
GGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTATGGTAGTTTCTTTAG163190.11924938144726907No Hit
CTAGGGATAACAGCGTAATCTTTTTGGAGAGTTCAAATCGATAAAAAGGT159600.11662602658854185No Hit
GGTGCAGAAGTTTTGCTGGAAAGTCTGTTCGACTTTTAATCTCTTACATG148000.10814944821493855No Hit
CTGTTCGACTTTTAATCTCTTACATGATCTGAGTTCAAACCGGCGTAAGC146100.10676104313650353No Hit
ATCTGAGTTCAAACCGGCGTAAGCCAGGTTGGTTTCTATCCTCAAAATTA141540.10342887094825948No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG194550.065.130811
ACATGGC201450.063.016633
CATGGCT217000.058.739174
GGCTACG228550.055.462267
GCTACGA227350.055.258858
ATGGCTA237100.053.779425
CTACGAT235250.052.6839529
TGGCTAC282100.045.4172256
GACATGG287450.044.114112
GGCCTCC31900.037.8652048
CGCTTGG39550.031.9671293
GCCTCCG39300.030.4961839
TGGCCTC39550.030.4222497
CCGCTTG44300.028.3273122
ACCGCTT48250.026.3005181
ACGATGG244050.024.76623710-11
TACGATG250900.024.56775510-11
CGATGGG239250.024.51661512-13
GCTTGGC53500.024.1588784
GATGGGA135800.019.55449312-13
Produced by FastQC (version 0.11.5)