FastQCFastQC Report
Thu 20 Oct 2022
C3-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC3-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22644025
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT378880.1673200767089773No Hit
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA279300.123343795990333No Hit
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA260350.11497514244927745No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG2515250.075.87271
ACATGGC2526750.075.6296843
CATGGCT2570900.074.488114
GACATGG2570200.074.352972
ATGGCTA2672500.071.5701455
TGGCTAC2758200.069.366836
GGCTACG2825500.067.461767
GCTACGA2889600.065.223568
CTACGAT2943850.063.0796479
TACGATG2885150.031.70909710-11
ACGATGG2890650.031.12440110-11
GGGGTTA143150.025.6423325
ATGGGGT469200.025.232953
GATGGGA1245050.024.68627712-13
CGATGGG3587700.024.37837612-13
CGATCCG161100.024.2439481
TGGGGTT269750.024.0444854
GATGGGG1386650.023.59405712-13
GATGGGT562900.023.5626222
GGGTTAG134300.023.2025326
Produced by FastQC (version 0.11.5)