FastQCFastQC Report
Thu 20 Oct 2022
C3-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC3-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences21570033
Sequences flagged as poor quality0
Sequence length100
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT521650.24184014924780134No Hit
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA340080.15766318020932096No Hit
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA309520.1434953762008616No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGC2921450.076.277573
CGACATG2920750.076.1703341
CATGGCT2962250.075.441174
GACATGG2972200.074.9862062
ATGGCTA3085600.072.3628855
TGGCTAC3172650.070.408526
GGCTACG3257450.068.354857
GCTACGA3320250.066.2607658
CTACGAT3372000.064.328119
TACGATG3302950.032.24593410-11
ACGATGG3292950.031.81932410-11
CGATCCG164150.027.1434651
GATGGGA1345900.026.17144812-13
CGATGGG4023250.025.34489412-13
GATGGGG1504800.025.14287612-13
ATGGGGG292450.023.85758414-15
GGGGTTA169200.023.8333345
ATGGGGT524750.023.7171973
CGACTTA144500.023.2235286
TGGGGTT301650.022.732644
Produced by FastQC (version 0.11.5)