FastQCFastQC Report
Thu 20 Oct 2022
C2-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC2-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9414189
Sequences flagged as poor quality0
Sequence length100
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT578380.6143704996787297No Hit
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA180310.19153004045276764No Hit
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG100970.10725299863854443No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGC1268650.084.5603563
CGACATG1269000.084.362961
CATGGCT1283050.083.834764
GACATGG1287050.083.336852
ATGGCTA1326250.080.979985
TGGCTAC1355100.079.273266
GGCTACG1381450.077.45847
GCTACGA1391000.075.612238
CTACGAT1402950.073.457289
CGATCCG108850.038.8608171
TACGATG1343650.037.03788410-11
ACGATGG1321900.036.65886310-11
GATCCGA128250.031.5898652
CGATGGG1582400.030.04180312-13
GATGGGG742750.029.98761212-13
ATCCGAC134800.029.5667653
GATGGGA531950.029.2982412-13
TCCGACT139150.028.3722614
ATGGGGG136900.028.03177614-15
ATGGGGT275100.027.23300714-15
Produced by FastQC (version 0.11.5)