FastQCFastQC Report
Thu 20 Oct 2022
C2-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC2-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9014164
Sequences flagged as poor quality0
Sequence length100
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTT538230.5970936406304567No Hit
CGATGGGGTTAGTTTTTATTTATTAATTTTTATTATTTTTTAAAAAATTA161050.17866326816330388No Hit
CGACATGGCTACGATGTGAACCACAGGAAGCGCAAGTCCGGCACTGTCGG112950.1253028012359216No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACATGGC1374800.085.9045643
CGACATG1376550.085.638291
CATGGCT1391750.085.084614
GACATGG1396750.084.578132
ATGGCTA1444200.081.919685
TGGCTAC1475750.080.181056
GGCTACG1497950.078.7605747
GCTACGA1513750.076.761758
CTACGAT1523750.074.5439
TACGATG1452900.037.7934510-11
ACGATGG1427850.037.41632510-11
CGATCCG102200.033.5254361
CGATGGG1717750.030.42447912-13
GATGGGA589350.030.09323912-13
GATGGGG801250.029.9480212-13
ATGGGGG148500.029.26027114-15
ATGGGGT274100.027.00656714-15
GATCCGA124300.026.4682222
ATGGGGA367700.026.3951614-15
GGGGTTA111800.026.14847816-17
Produced by FastQC (version 0.11.5)