FastQCFastQC Report
Thu 20 Oct 2022
C1-MGI-2_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC1-MGI-2_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8464971
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA342980.40517563497854864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG1892650.069.455161
ACATGGC1919350.068.608963
GACATGG1920750.068.55652
CATGGCT1958550.067.372544
ATGGCTA2016650.065.333655
TGGCTAC2043800.064.4588556
GGCTACG2086200.062.876197
GCTACGA2136950.060.9694638
CTACGAT2175950.059.33679
TACGATG2133650.030.0043110-11
ACGATGG2143900.029.55832710-11
GATGGGA776750.027.82484612-13
GATGGGG598300.027.3492412-13
CGATGGG2399650.025.83516312-13
ATGGGGG114150.025.0131414-15
ATGGGGT211250.024.68473414-15
GATGGGT476150.023.69001212-13
ATGGGAT322700.023.6602114-15
ATGGGGC88450.023.06161714-15
ATGGGCA149900.022.98265514-15
Produced by FastQC (version 0.11.5)