FastQCFastQC Report
Thu 20 Oct 2022
C1-MGI-1_parsed.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameC1-MGI-1_parsed.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9352668
Sequences flagged as poor quality0
Sequence length100
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGACATGGCTACGATGGGCGACATGGCTACGATGGGCGACATGGCTACGA394220.4215053928996518No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACATG2176500.069.4818341
ACATGGC2205000.068.730843
GACATGG2213900.068.460912
CATGGCT2254000.067.4535454
ATGGCTA2315950.065.57215
TGGCTAC2350500.064.610256
GGCTACG2395100.063.1461337
GCTACGA2450000.061.3513038
CTACGAT2492350.059.8147549
TACGATG2443900.030.18008210-11
ACGATGG2458250.029.75535410-11
GATGGGA861950.028.8668712-13
GATGGGG669000.028.22107712-13
CGATGGG2682250.026.59843312-13
ATGGGGG123500.026.3732814-15
ATGGGGT238700.024.99643914-15
GATGGGT513050.024.86268612-13
ATGGGAT347500.024.53467814-15
ATGGGCA164150.023.87937714-15
ATGGGGA315850.023.36979714-15
Produced by FastQC (version 0.11.5)