FastQCFastQC Report
Fri 29 Jan 2016
trimmed.H61RP2_11895_TGACCA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed.H61RP2_11895_TGACCA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34439444
Sequences flagged as poor quality0
Sequence length25-44
%GC65

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGATGCGTGCATTTATCAGA22923366.65613533133694No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC16625254.827386295783405No Hit
GGGGGGGATGCGTGCATTTATCAGAT6305101.8307786850449734No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCC5835681.6944756715584608No Hit
GGGGCGCGGGACATGTGGCGTACGGAAG5619921.6318265765266131No Hit
GGGGCGCGGGACATGTGGCGTACGGAAGA5252811.5252307789870243No Hit
GGGGGGATGCGTGCATTTATCAGAT5196901.5089964867028631No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT5075931.4738710648174227No Hit
ACTGCATAATTTGTGGTAGTGGGGG5072371.4728373663639867No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTG4483241.3017747905570136No Hit
GGGCGCGGGACATGTGGCGTACGGAAGA4003521.162481020309155No Hit
GACTGCATAATTTGTGGTAGTGGGGG3923101.1391298883919263No Hit
GGGCGCGGGACATGTGGCGTACGGAAG3704641.0756968085779781No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCCC3682811.0693581464323292No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCC3616741.0501737484495974No Hit
CGACTGCATAATTTGTGGTAGTGGGGG3083480.8953338503374212No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAAT2994840.8695959203057981No Hit
ACTGCATAATTTGTGGTAGTGGGGGA2943870.8547960298081467No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCT2928250.8502605326613286No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC2806370.814870878867847No Hit
CGGGGCGCGGGACATGTGGCGTACGGAAG2448180.7108651347565309No Hit
CGGGGCGCGGGACATGTGGCGTACGGAAGA2417970.7020932161390294No Hit
CCGCCTGGATACCGCAGCTAGGAATAATGGAA2397560.6961668719158184No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCC2190190.6359539370031642No Hit
GGGGCGCGGGACATGTGGCGTACGGAA2171740.6305967076588113No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA2085960.6056892207667465No Hit
GACTGCATAATTTGTGGTAGTGGGGGA2080910.6042228788594844No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGG2041370.5927418572727249No Hit
GGGGGGCCCAAGTCCTTCTGATCGAGGCCC2031670.5899253193518454No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCCC2010540.5837899125200744No Hit
GGGGGGGGATGCGTGCATTTATCAGA1987280.5770360288046462No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCC1888330.548304438364336No Hit
GTGGGGGGCCCAAGTCCTTCTGATCGAGGCCC1825750.5301334133036527No Hit
TCCGCCGAGGGCGCACCACCGGCCCGTCTCGC1769400.5137713605364825No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1738040.5046655224747532No Hit
CTCGCCCGTCACGCACCGCACGTTCGTG1704770.49500508777087115No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCCC1651880.479647696983726No Hit
GGGCGCGGGACATGTGGCGTACGGAA1556380.4519178648760996No Hit
CTCGGTTGGCCTCGGATAGCCGGTCCCC1540850.4474085005553516No Hit
TGGGGGGCCCAAGTCCTTCTGATCGAGGCC1509220.43822426401541215No Hit
TGGTGACTCTAGATAACCTCGGGCCGATCGCAC1397250.4057121247369731No Hit
GCGCGGGACATGTGGCGTACGGAAGA1395860.4053085177565584No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGT1368520.3973699459259563No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG1307190.3795618767829121No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC1302120.3780897275809679No Hit
GCGCGGGACATGTGGCGTACGGAAG1246610.36197158119045125No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCC1213920.3524795580323538No Hit
ACCGGGGTCCGGTGCGGAGTGCCCTTC1147030.333057060967651No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGA1114750.3236840873505391No Hit
CGGGGCGCGGGACATGTGGCGTACGGAA1110770.32252843570877626No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTGAA1103190.3203274710242128No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGG1092930.3173483288522312No Hit
GGGGCGCGGGACATGTGGCGTACGG1081290.313968483347176No Hit
GGGGGGGCCGGGCCACCCCTCCCACGGCGC1066320.30962172327753024No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTG1044540.30329757936858676No Hit
GGCGCGGGACATGTGGCGTACGGAAGA1024200.29739156067676353No Hit
CTCGCCCGTCACGCACCGCACGTTCG999140.2901150204399351No Hit
CTCCCGGGGCTACGCCTGTCTGAGCGTCGCT980290.28464164520193763No Hit
CTCGCCCGTCACGCACCGCACGTTCGT963360.2797257702534338No Hit
GGCGGCGTCCGGTGAGCTCTCGCTGGCC934950.27147650815733265No Hit
TCCCGGGGCTACGCCTGTCTGAGCGTCGCTT899650.26122663304320476No Hit
CGCGGGACATGTGGCGTACGGAAGA870690.25281767034334235No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATTGC855150.24830540237525323No Hit
GGCGCGGGACATGTGGCGTACGGAAG841200.244254814334401No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGC789520.22924876487553053No Hit
GGGGCGCGGGACATGTGGCGTACGGA709460.2060021642625822No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC702510.20398412936050883No Hit
GGGGGGGATGCGTGCATTTATCAGATC684500.19875466049916485No Hit
ATTCAACCCGGCGGCGGGTCCGGCCGTGT682520.19817973832562452No Hit
GACCGGGGTCCGGTGCGGAGTGCCCTTC676110.19631850037997128No Hit
CTGCATAATTTGTGGTAGTGGGGGA675720.1962052581336679No Hit
GATGGTTTAGTGAGGCCCTCGGATCGGCC665690.19329289985053186No Hit
GGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC649090.18847284526428476No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCGA645660.18747689422628308No Hit
GAGATACCATGATCACGAAGGTGGT639540.18569986205352212No Hit
GCTGAGAAGACGGTCGAACTTGACTATC624080.18121082326416185No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGG584830.16981400745029449No Hit
GGGGGGATGCGTGCATTTATCAGATC576820.16748818593006323No Hit
GACTGCATAATTTGTGGTAGTGGGG570990.1657953595301945No Hit
TAAACCATTCGTAGACGACCTGCTT567300.1647239136613239No Hit
GGTCCGATGGTAGTGGGTTATCAGAA552110.16031327335017373No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGCG551070.16001129402669798No Hit
CGACTGCATAATTTGTGGTAGTGGGG544620.1581384414916803No Hit
GCCTCGGTTGGCCTCGGATAGCCGGTCCC543920.15793518617780242No Hit
CGGGGGGGATGCGTGCATTTATCAGA543770.1578916314676857No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT541350.15718894881113643No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGCC527500.15316739724369532No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA525270.1525198838866272No Hit
CCTCGCCCGTCACGCACCGCACGTTCGT523280.15194205806574576No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGG515810.14977303350193458No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA512120.148701587633064No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAAG503640.14623929468780042No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCC495170.14377990538987795No Hit
GGAGATACCATGATCACGAAGGTGGT491070.14258940998002173No Hit
CCTCGCCCGTCACGCACCGCACGTTCG491020.1425748917433162No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGG489950.14226420147781713No Hit
TGAGAGGTGTAGAATAAGTGGGAGGCCC488820.14193608932827137No Hit
GGATGGTTTAGTGAGGCCCTCGGATCGGCC488350.14179961790323908No Hit
TCGGTTGGCCTCGGATAGCCGGTCCCC486170.1411666227828765No Hit
GGGGGGGGATGCGTGCATTTATCAGAT484840.14078043768650852No Hit
AGCCTCGGTTGGCCTCGGATAGCCGGTCCCC482430.14008065867730035No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC474600.13780710280920913No Hit
GTCCGCCGAGGGCGCACCACCGGCCCGTCTCGC473410.1374615687756167No Hit
CTCGCTTCTGGCGCCAAGCGCCCGGCCG467080.13562356000869236No Hit
GGGCTTCTGTCGTGAGTGGCACACGTAGGG461230.13392492631414143No Hit
GGCGCGGGACATGTGGCGTACGGAA456770.13262989960000515No Hit
CCTCGGTTGGCCTCGGATAGCCGGTCCCCC449170.1304231276207595No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGG445620.12939233281466447No Hit
CGGGGCGCGGGACATGTGGCGTACGGA440100.12778951948237027No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC437220.1269532690481298No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAC436170.12664838607731296No Hit
GCGACCTCAGATCAGACGTGGCGACCCGCTG431960.12542595054670452No Hit
GGCGGGGCGCGGGACATGTGGCGTACGGAA420380.12206352692569601No Hit
GGGCGCGGGACATGTGGCGTACGGA415120.12053620842427072No Hit
CAACTTCTTAGAGGGACAAGTGGCGT413720.12012969779651496No Hit
GGGCGGGTCGCGCCGTCGGGCCCGGGGG413520.12007162484969269No Hit
TAAACCATTCGTAGACGACCTGCTTC410100.11907857745903215No Hit
TCGCCCGTCACGCACCGCACGTTCGTGGG407800.11841073857057623No Hit
CCTCGCCCGTCACGCACCGCACGTTCGTGGGGA406790.11811747018912384No Hit
CTCGCCCGTCACGCACCGCACGTTCGTGGGGA401400.1165524042722641No Hit
GCGGCGTCCGGTGAGCTCTCGCTGGCC401180.11648852403075961No Hit
TCGACTGCATAATTTGTGGTAGTGGGG401060.11645368026266627No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCGA398530.11571905748536475No Hit
CGGTTGGCCTCGGATAGCCGGTCCCC389160.11299833992674214No Hit
GGGCGCGGGACATGTGGCGTACGGAAGAC381460.11076253147408535No Hit
GGGGCGCGGGACATGTGGCGTACGGAAGAC381010.11063186734373527No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG359570.10440644744438965No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA357930.10393024928044714No Hit
ACTGCATAATTTGTGGTAGTGGGGGAC345170.10022519527318735No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGCCATA502.0332664E-8518253.838
AGTTGCC959.933816E-628217.08635
ACCGCTC7850.014727.44832
ACCGTTC406.603952E-511105.43833
TTGCCAT12058.266533E-59216.13237
GTATCAC551.2485319E-48076.682633
CGACCGC47050.07668.48631
GTTGCCA11351.920741E-46226.517636
TACCGAT4402.1307847E-46092.32535
CCGCTCT3650.06085.17233
GACCGCT9750.04945.966331
CAAGTTG1054.5500972E-44230.64333
GACCGTT505.1404885E-43986.567932
CGTATCA607.4020575E-43322.139632
ATTTGAC1256.6794564E-73189.254232
CCGGCAA1750.00126378462538.38633
ATTGCAA3500.02538.38633
AGAGGGT4550.00159917022248.064534
TAGGAAG2050.00173414492166.914833
GGCGGCT4850.00181696732109.00934
Produced by FastQC (version 0.11.2)