FastQCFastQC Report
Fri 29 Jan 2016
trimmed.H181RP_11896_ACAGTG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed.H181RP_11896_ACAGTG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39441134
Sequences flagged as poor quality0
Sequence length25-44
%GC58

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCATAATTTGTGGTAGTGGGGG35873869.095544768058646No Hit
TCGACTGCATAATTTGTGGTAGTGGGGG35609449.028503085129348No Hit
ACTGCATAATTTGTGGTAGTGGGGGA21434025.434432995765285No Hit
GACTGCATAATTTGTGGTAGTGGGGG20189615.118922290621765No Hit
CGACTGCATAATTTGTGGTAGTGGGGG15424113.9106659560042063No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGT15347623.891272497388133No Hit
GACTGCATAATTTGTGGTAGTGGGGGA12069203.0600540035182555No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC10437372.646315899537777No Hit
CGACTGCATAATTTGTGGTAGTGGGGGA10136762.5700985169442645No Hit
TCGACTGCATAATTTGTGGTAGTGGGG7276701.844952023945356No Hit
ATTCAACCCGGCGGCGGGTCCGGCCGTGT6371591.6154682570739474No Hit
CTGCATAATTTGTGGTAGTGGGGGA5688701.4423266836090463No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGC3453040.8754920687625259No Hit
ACTGCATAATTTGTGGTAGTGGGGGAC3453020.8754869979144108No Hit
CGACTGCATAATTTGTGGTAGTGGGG3318910.8414844258788299No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT3318310.841332300435378No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTG2868050.7271722968208774No Hit
GACTGCATAATTTGTGGTAGTGGGG2781880.7053245477171118No Hit
TCGACTGCATAATTTGTGGTAGTGGGGGA2685190.680809532504821No Hit
GGTCCGATGGTAGTGGGTTATCAGAA2615540.6631503039441006No Hit
GGAGATACCATGATCACGAAGGTGGT2335130.5920544779468055No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTGT2150180.5451618100027246No Hit
GGGGGGGATGCGTGCATTTATCAGA2045360.5185854950316591No Hit
GACTGCATAATTTGTGGTAGTGGGGGAC1992320.5051376058305018No Hit
GAGATACCATGATCACGAAGGTGGT1768060.4482781859162569No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1669030.42316988147450324No Hit
GGGGGGGCCGGGCCACCCCTCCCACGGCGCGACC1208220.3063350054793049No Hit
GGATTCAACCCGGCGGCGGGTCCGGCCGTG1200820.3044587916767302No Hit
TGGTCCGATGGTAGTGGGTTATCAGAA1171930.2971339515745161No Hit
TCGCTTCTGGCGCCAAGCGCCCGGCCG1117290.2832803945241534No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC1079310.2736508539536414No Hit
CGACTGCATAATTTGTGGTAGTGGGGGAC1065650.2701874646910507No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC1035430.2625254131891847No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGA955410.2422369498808021No Hit
CGCATGAAGATTACGGAAAAGAAGCGT878920.22284349126472885No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTA872620.2212461741084828No Hit
GGGGGGGATGCGTGCATTTATCAGAT802920.20357426842747472No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA801790.20328776550897343No Hit
ATTCAACCCGGCGGCGGGTCCGGCCGTG733310.18592518156298446No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG732890.18581869375256807No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCG730780.18528371927642853No Hit
GGGGGGGCCGGGCCACCCCTCCCACGGCGC713000.18077573530213406No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA689340.17477692198200995No Hit
CTGCATAATTTGTGGTAGTGGGGGAC584630.14822849667557733No Hit
GCTGGTCCGATGGTAGTGGGTTATCAGAA558870.1416972443033712No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT557010.14122565542867No Hit
GGCCCGGCGGGGAAGGTGGCTCGGGGGGC537750.13634242869386057No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCGCGAC510450.12942072101679428No Hit
TTCAACCCGGCGGCGGGTCCGGCCGTGT510200.12935733541535596No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGCC498600.12641624350861716No Hit
GATTCAACCCGGCGGCGGGTCCGGCCGTGTC490590.12438536883853288No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT466010.11815329650511569No Hit
GGGAGATACCATGATCACGAAGGTGGT456920.11584859603681781No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGC456570.11575985619480414No Hit
TGCATAATTTGTGGTAGTGGGGGAC440530.1116930360065205No Hit
GGGGGGATGCGTGCATTTATCAGAT438890.11127722646108501No Hit
CAGTGCGCCCCGGGCGGGTCGCGCCGTCGGG415060.10523531093198284No Hit
TGGGGGGGCCGGGCCACCCCTCCCACGGCG396950.1006436579637898No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTACTCA1952.1296932E-519181.25438
CGAATAT1102.4265319E-89595.97636
GCTAATC4159.6455835E-59012.87838
GACTTTT5201.5143683E-47192.969738
TTTACTC2453.301739E-44945.554737
TCGCATT2658.1699094E-54572.30537
GGAATCA8704.2387584E-44299.24638
GGCTAAT3004.950401E-44038.869137
AGTGTGT19100.03916.591338
ATCGGTC9905.488606E-43778.125538
TGAGATT15500.03571.903333
TCGAAGT3406.358378E-43563.708537
CCAGACC10651.6370905E-113512.060538
GTAAATC1557.020905E-43405.023436
CGAATTG3155.6966564E-73350.975336
ATTTATT3807.9423026E-43188.58137
AACGCAG12208.3347975E-43065.85638
GAAGGGT13009.463601E-42877.18838
ATAGGTC1850.00100013122852.857436
TATTGCC1950.00111116172706.557136
Produced by FastQC (version 0.11.2)