FastQCFastQC Report
Fri 29 Jan 2016
trimmed.H1201RP_11897_ACTTGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenametrimmed.H1201RP_11897_ACTTGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32501482
Sequences flagged as poor quality0
Sequence length25-44
%GC61

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ACTGCATAATTTGTGGTAGTGGGGG383109111.787434800665398No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCGC23226477.146280283465228No Hit
ACTGCATAATTTGTGGTAGTGGGGGA16922955.206824107282246No Hit
GCCGAGGGCGCACCACCGGCCCGTCTCGC9559002.9410966552232907No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCG9216732.8357876111618543No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGA8943322.751665293293395No Hit
CTGCATAATTTGTGGTAGTGGGGGA8052892.4776993246031056No Hit
GACTGCATAATTTGTGGTAGTGGGGG6974592.145929837907084No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGG5355731.6478417814916873No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCGA4242951.305463547785298No Hit
CCGAGGGCGCACCACCGGCCCGTCTCGC4171071.28334763319408No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGCG3915111.204594301269093No Hit
GGTCCGATGGTAGTGGGTTATCAGAA3354541.0321190892156857No Hit
GACTGCATAATTTGTGGTAGTGGGGGA3216370.9896071816048265No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGC2852300.8775907510925194No Hit
GAGATACCATGATCACGAAGGTGGT2272920.6993281106381549No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGGG2030380.6247038211980611No Hit
GACTGCATAATTTGTGGTAGTGGGG1917350.589926945485132No Hit
CTCGCCGAATCCCGGGGCCGAGGGAGC1800110.5538547442236634No Hit
CGCATGAAGATTACGGAAAAGAAGCGT1690670.520182433527185No Hit
TGCGCCGCGACCGGCTCCGGGACGGCTGGGA1616700.49742347133586096No Hit
GAGGGCGCACCACCGGCCCGTCTCGC1436820.4420783027678553No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGA1296790.39899411356072934No Hit
TTGAACCCCATTCGTGATGGGGATCGGGGATT1294700.398351065960623No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGG1232150.37910578969906666No Hit
ACTGCATAATTTGTGGTAGTGGGGGAC1159690.3568114217068625No Hit
GGAGATACCATGATCACGAAGGTGGT1144700.35219932432619533No Hit
TGAACCCCATTCGTGATGGGGATCGGGGATT1132030.34830104054947403No Hit
AGGGCGCACCACCGGCCCGTCTCGC1082160.33295712484741463No Hit
TGGTCCGATGGTAGTGGGTTATCAGAA1077460.33151103694286926No Hit
CTGGTCCGATGGTAGTGGGTTATCAGAA916490.28198406460357717No Hit
GTCCGATGGTAGTGGGTTATCAGAA896160.2757289652207244No Hit
CGCCGAGGGCGCACCACCGGCCCGTCTCG779420.2398106030980372No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGGA764320.23516466110683815No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGC751360.2311771506296236No Hit
TGTGTGACTGAAAGGTATTTTCTGAGC707430.21766084389628756No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTT678970.2089043201168488No Hit
CGCCGCGACCGGCTCCGGGACGGCTGGG672950.2070520968859205No Hit
TGCATAATTTGTGGTAGTGGGGGAC596190.18343471230019603No Hit
GCACTCGCCGAATCCCGGGGCCGAGGGAGC595060.18308703584655No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGGG547360.168410782006802No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAG530850.16333101364423935No Hit
GGGGGGGATGCGTGCATTTATCAGA484430.14904858799977183No Hit
CACTCGCCGAATCCCGGGGCCGAGGGAGCG483120.1486455294561645No Hit
CTGCATAATTTGTGGTAGTGGGGGAC482290.14839015648578732No Hit
ACGATGCCGACCGGCGATGCGGCGGCGTTA478490.1472209790310485No Hit
ACTCGCCGAATCCCGGGGCCGAGGGAGCGAG473290.14562105198772166No Hit
CGACTGCATAATTTGTGGTAGTGGGGG432030.13292624625547844No Hit
GGGAGATACCATGATCACGAAGGTGGT419470.12906180708928905No Hit
ACCGGGGTTCGAATCCCCGTAGGGACGCCA395350.12164060703447306No Hit
CCGCCGAGGGCGCACCACCGGCCCGTCTCGC381620.11741618428353513No Hit
GCGCCGCGACCGGCTCCGGGACGGCTGGGAA357350.10994883248708474No Hit
CTCGCCGAATCCCGGGGCCGAGGGAG352790.10854581954139815No Hit
GGTGCGCCGCGACCGGCTCCGGGACGGCTGG348980.10737356530388369No Hit
GTTCAACTTTAAATTTGCCCACAGAAC344650.10604131836203654No Hit
GTGCGCCGCGACCGGCTCCGGGACGGCTGG339890.10457676976083737No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTCTAC2355.1043935E-637473.48437
GCCTGCC39800.017701.04138
AGGTCAC7955.8415597E-511077.06737
CCGTTCC8256.2907406E-510674.26437
AACCCAT2552.5240303E-45638.26736
TGCCTAC17852.9447378E-44933.483437
GTGAAGT22151.891749E-103975.74237
CGGCACA3655.17105E-43939.06336
AAGTCTA3701.2216529E-53885.832836
GCACTTT25606.05663E-43439.948237
ACGAAGT4257.010665E-43382.960236
CGCCTGC31700.02778.002437
TCCGTTC5250.00106974612738.586736
TCCGGAC5700.0012609652522.382836
CTGTAAC6700.00174214252145.907736
GCGTGTT6900.0018476872083.707336
ACCTACA8200.00260935281753.363536
GCAAACC8400.00273816611711.616836
AAATGCC9400.0034287641529.529936
GGCAAGC9900.0038031431452.280836
Produced by FastQC (version 0.11.2)