FastQCFastQC Report
Tue 20 Oct 2015
11361_CTTGTA_L002_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename11361_CTTGTA_L002_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65772932
Sequences flagged as poor quality0
Sequence length75
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATC6556510.9968401591098295No Hit
CAACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCAT6305740.9587135327949194No Hit
CAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAATTACGCTG5450670.8287102055903482No Hit
TTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGT4607150.7004629199136204No Hit
CCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTA4332820.6587542729583653No Hit
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAA4025000.6119538657635027No Hit
CTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATATCTTAATC3891430.5916461197138057No Hit
CTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAATT3183540.48401977883546377No Hit
GCTACCTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGGTTAGACTTTATATATAATTCA3139200.47727840382727654No Hit
CTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATG2859430.43474266891431873No Hit
GTCCAACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGC2829910.4302545004379614No Hit
CTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAAT2786560.4236636432750178No Hit
CTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAATATTTC2631170.4000384231008586No Hit
TAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATATCTTAATCCAACATCGAGG2306820.35072482400510896No Hit
TCCAACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCC2127860.32351606280832973No Hit
GCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAA2009350.3054980124650669No Hit
GGGTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAAC1974610.30021620444106095No Hit
CTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAAT1927500.2930536835426464No Hit
CTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTT1917330.2915074547687794No Hit
CTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACA1908310.29013606995655905No Hit
GTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATAT1823840.277293400878039No Hit
GTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAG1784660.27133654312384914No Hit
TTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGTA1539120.23400507673886273No Hit
TTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAATATTTCG1514830.23031206819242905No Hit
CTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGGTTAGACTTTATATATAATTCAAAAAG1444250.21958121009414633No Hit
CCAACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCA1396320.21229401784916627No Hit
CCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAATTACGCT1390590.2114228388054831No Hit
CGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAATAT1273890.19367997765402947No Hit
TCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCA1231700.18726548483500782No Hit
CCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAATTACGCTGTTATCCCTAAAGTAACT1207840.18363785272640729No Hit
AAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGT1148760.17465543424459168No Hit
ATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCATTTAAA1089080.16558179282626478No Hit
CCCCAATAAAATATTTTTATTTATTAAAATTTAATTAATCTATATAATTAAAATAAAAAAAAATATAAAGATTTA946850.14395739572625407No Hit
CTACCTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGGTTAGACTTTATATATAATTCAA945210.14370805303312312No Hit
TCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAATA801760.12189816929553939No Hit
AACGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAA767230.11664828929931235No Hit
TGCTACCTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGGTTAGACTTTATATATAATTC757490.11516743696327846No Hit
AAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAATTACGCTGT745540.11335058014442781No Hit
AACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCATT743760.11307995209944419No Hit
GGGGTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAA740500.11258430747773263No Hit
ATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCA717760.10912695818395325No Hit
TTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTA716360.1089141046654268No Hit
TTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATATC694230.10554949869043392No Hit
CAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAA676830.10290403353160538No Hit
CCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTTCA658400.10010196899843238No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTTT1310650.037.148211
CCTTTCG1440250.033.8603743
TACGCTG1187000.033.31149369
TCCTTTC1494150.032.964872
ATTACGC1341850.029.5798967
CCCTAAA311550.028.73867263
CCCCAAT288300.028.6588331
AATTACG1394150.028.48762966
CGCTACC104850.028.4451121
TTACGCT1431250.027.74792168
GGGGTCT242950.027.5000931
CTTTCGT1796300.027.3174464
CCCAATA291750.027.2563532
TCCCTAA336550.026.61417662
ATCCCTA371350.024.64301761
CAACCAT1940350.023.6285691
ACCATTC2022900.023.5699393
CCATTCA2125050.023.311684
AAATTAC1714950.023.3014365
TTATCCC393200.023.19268259
Produced by FastQC (version 0.11.2)