FastQCFastQC Report
Tue 20 Oct 2015
11358_GTGAAA_L001_R1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename11358_GTGAAA_L001_R1_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56248404
Sequences flagged as poor quality0
Sequence length75
%GC47

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATC3703340.658390236281193No Hit
CAACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCAT2980940.5299599256185118No Hit
CTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATATCTTAATC1969390.350123711954565No Hit
CCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTA1722000.3061420196029029No Hit
CTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAAT1626860.2892277619112535No Hit
CTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATG1597600.2840258365375131No Hit
GTCCAACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGC1569600.2790479175195798No Hit
CGGCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAA1555400.27652340144619925No Hit
CAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAATTACGCTG1542430.27421755824396365No Hit
TTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGT1541670.2740824432991912No Hit
CTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAATT1485760.2641426057173107No Hit
CTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAATATTTC1478040.2627701223309376No Hit
GCTACCTTTGCACAGTCAAAATACTGCGGCCATTTAAAATTTTCAGTGGGCAGGTTAGACTTTATATATAATTCA1325750.23569557635804206No Hit
GGGTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAAC1253630.22287387923042226No Hit
CTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTT1076650.19140987538064191No Hit
CTGGCTTACACCGGTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACA1067980.18986849831330324No Hit
GTCTTCTCGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAG1062030.1888106905219924No Hit
TAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATATCTTAATCCAACATCGAGG972580.17290801708791595No Hit
GCTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAA962940.1711941906831703No Hit
CCCCAATAAAATATTTTTATTTATTAAAATTTAATTAATCTATATAATTAAAATAAAAAAAAATATAAAGATTTA906140.16109612638964832No Hit
GTTTGAACTCAGATCATGTAAGAATTTAAAAGTCGAACAGACTTAAAATTTGAACGGCTACACCCAAAATTATAT848210.15079716750718827No Hit
CTACACCCAAAATTATATCTTAATCCAACATCGAGGTCGCAATCTTTTTTATCGATATGAACTCTCCAAAAAAAT812800.14450187777772325No Hit
TCCAACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCC753070.13388290981553894No Hit
CGTCTTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAATAT687460.12221857885958863No Hit
CCAACCATTCATTCCAGCCTTCAATTAAAAGACTAATGATTATGCTACCTTTGCACAGTCAAAATACTGCGGCCA680590.12099721087197425No Hit
TTTTAAATAAATTTTAGCTTTTTGACTAAAAAATAAAATTCTATAAAAATTTTAAATGAAACAGTTAATATTTCG632660.11247608021020472No Hit
TCCTTTCGTACTAAAATATCACAATTTTTTAAAGATAGAAACCAACCTGGCTTACACCGGTTTGAACTCAGATCA565900.100607299008875No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCTTT746000.037.3412131
CCTTTCG758200.036.021133
TCCTTTC820850.033.9384042
TACGCTG407300.028.19309469
CCCCAAT292750.027.4316711
CTTTCGT1068650.026.2780194
ATTACGC442600.026.00090667
CGTACTA1056200.025.8219328
CCCAATA299700.024.8459262
TTCGTAC1114250.024.5966666
GTACTAA1113700.024.5845349
AATTACG472950.024.38423366
TTTCGTA1130550.024.3187755
ACCATTC1060400.021.9102553
TATGCCG80500.021.77823348
CAACCAT1038400.021.6329141
GGGGTCT217050.021.5017591
TTACGCT542200.021.49682668
TACTAAA1320900.020.9990610
CCATTCA1157250.020.8526554
Produced by FastQC (version 0.11.2)