[2024-04-23 12:32:03] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2024-04-23 12:32:03] Checking for Bowtie Bowtie 2 not found, checking for older version.. Bowtie version: 1.1.1.0 [2024-04-23 12:32:03] Checking for Bowtie index files (genome).. [2024-04-23 12:32:03] Checking for reference FASTA file [2024-04-23 12:32:03] Generating SAM header for /no_backup/ldicroce/eblanco/molbio/indexes/mm10/genome [2024-04-23 12:32:07] Reading known junctions from GTF file [2024-04-23 12:32:11] Preparing reads left reads: min. length=50, max. length=50, 46035534 kept reads (438 discarded) [2024-04-23 12:39:12] Building transcriptome data files /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_PrEW3_08588/tmp/refGene [2024-04-23 12:39:25] Building Bowtie index from refGene.fa [2024-04-23 12:50:12] Mapping left_kept_reads to transcriptome refGene with Bowtie [2024-04-23 13:25:07] Resuming TopHat pipeline with unmapped reads [2024-04-23 13:25:07] Mapping left_kept_reads.m2g_um to genome genome with Bowtie [2024-04-23 13:29:10] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie (1/2) [2024-04-23 13:33:54] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie (2/2) [2024-04-23 13:38:27] Searching for junctions via segment mapping [2024-04-23 13:40:41] Retrieving sequences for splices [2024-04-23 13:42:05] Indexing splices [2024-04-23 13:42:20] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie (1/2) [2024-04-23 13:42:57] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie (2/2) [2024-04-23 13:43:33] Joining segment hits [2024-04-23 13:45:11] Reporting output tracks ----------------------------------------------- [2024-04-23 13:57:43] A summary of the alignment counts can be found in /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_PrEW3_08588/align_summary.txt [2024-04-23 13:57:43] Run complete: 01:25:39 elapsed