[2024-04-23 12:32:03] Beginning TopHat run (v2.0.14) ----------------------------------------------- [2024-04-23 12:32:03] Checking for Bowtie Bowtie 2 not found, checking for older version.. Bowtie version: 1.1.1.0 [2024-04-23 12:32:03] Checking for Bowtie index files (genome).. [2024-04-23 12:32:03] Checking for reference FASTA file [2024-04-23 12:32:03] Generating SAM header for /no_backup/ldicroce/eblanco/molbio/indexes/mm10/genome [2024-04-23 12:32:06] Reading known junctions from GTF file [2024-04-23 12:32:10] Preparing reads left reads: min. length=50, max. length=50, 47398751 kept reads (445 discarded) [2024-04-23 12:38:26] Building transcriptome data files /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_PrEW1_08587/tmp/refGene [2024-04-23 12:38:39] Building Bowtie index from refGene.fa [2024-04-23 12:50:29] Mapping left_kept_reads to transcriptome refGene with Bowtie [2024-04-23 13:20:21] Resuming TopHat pipeline with unmapped reads [2024-04-23 13:20:21] Mapping left_kept_reads.m2g_um to genome genome with Bowtie [2024-04-23 13:24:44] Mapping left_kept_reads.m2g_um_seg1 to genome genome with Bowtie (1/2) [2024-04-23 13:29:30] Mapping left_kept_reads.m2g_um_seg2 to genome genome with Bowtie (2/2) [2024-04-23 13:33:57] Searching for junctions via segment mapping [2024-04-23 13:36:17] Retrieving sequences for splices [2024-04-23 13:37:47] Indexing splices [2024-04-23 13:38:02] Mapping left_kept_reads.m2g_um_seg1 to genome segment_juncs with Bowtie (1/2) [2024-04-23 13:38:44] Mapping left_kept_reads.m2g_um_seg2 to genome segment_juncs with Bowtie (2/2) [2024-04-23 13:39:26] Joining segment hits [2024-04-23 13:41:08] Reporting output tracks ----------------------------------------------- [2024-04-23 13:54:36] A summary of the alignment counts can be found in /no_backup/ldicroce/eblanco/molbio/02_map_files/mm10/RNA_PrEW1_08587/align_summary.txt [2024-04-23 13:54:36] Run complete: 01:22:33 elapsed