FastQCFastQC Report
Tue 25 Mar 2014
6492_ACTTGA_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 6492_ACTTGA_R2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 144586011
Filtered Sequences 0
Sequence length 100
%GC 48

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 45166050 3.962344 6.0516043 2
GAAGA 48519365 2.8601081 6.8298783 2
GGAAG 33236030 2.1084003 6.316422 1
GAAAA 36736930 2.0123038 5.6196 2
GGAGA 30862370 1.9578221 5.1631947 1
GGAAA 30366190 1.790019 6.022015 1
GTTTT 18313075 1.5505748 5.3249655 1
GTTTG 15552120 1.270903 5.0560913 1
Produced by FastQC (version 0.10.1)