##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	6492_ACTTGA_R2.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	144586011	
Filtered Sequences	0	
Sequence length	100	
%GC	48	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.885527487164715	34.0	31.0	34.0	30.0	34.0
2	31.965935321363837	34.0	31.0	34.0	30.0	34.0
3	31.978907876502657	34.0	31.0	34.0	30.0	34.0
4	35.39680210141491	37.0	35.0	37.0	33.0	37.0
5	35.29918193814753	37.0	35.0	37.0	33.0	37.0
6	35.31525236559711	37.0	35.0	37.0	33.0	37.0
7	35.261209696144114	37.0	35.0	37.0	33.0	37.0
8	35.27839003041588	37.0	35.0	37.0	33.0	37.0
9	37.05645858782286	39.0	38.0	39.0	34.0	39.0
10-14	37.29115204374786	39.4	38.2	39.4	34.0	39.4
15-19	38.39079771140515	41.0	39.0	41.0	34.0	41.0
20-24	38.31867714505243	40.8	38.8	41.0	34.0	41.0
25-29	38.12275464878826	40.0	38.0	41.0	34.0	41.0
30-34	37.865294886654006	40.0	38.0	41.0	33.0	41.0
35-39	37.50005457996902	40.0	38.0	41.0	32.6	41.0
40-44	37.38588037400105	40.0	37.4	41.0	32.4	41.0
45-49	37.10157482386038	40.0	36.6	41.0	31.8	41.0
50-54	36.02654424154492	38.6	35.0	40.2	30.2	40.6
55-59	36.24812151709476	39.0	35.0	41.0	30.8	41.0
60-64	35.56408426953559	37.6	35.0	40.2	29.8	41.0
65-69	34.83608229567935	36.4	34.6	39.4	29.0	41.0
70-74	34.256177925816075	35.2	34.2	38.4	29.0	40.6
75-79	33.53926868623549	35.0	34.0	36.6	29.0	39.0
80-84	32.856004209148566	35.0	34.0	35.6	28.8	37.2
85-89	32.29965772691523	35.0	33.6	35.0	27.2	36.2
90-94	31.734873692587037	35.0	33.0	35.0	25.6	35.6
95-99	31.21928914409292	35.0	33.0	35.0	24.0	35.0
100	29.578094128345516	34.0	30.0	35.0	17.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1995718.0
3	278340.0
4	172677.0
5	165625.0
6	175574.0
7	173244.0
8	181263.0
9	201719.0
10	213347.0
11	221144.0
12	228857.0
13	232438.0
14	215873.0
15	212126.0
16	220138.0
17	233326.0
18	244120.0
19	256800.0
20	276042.0
21	302435.0
22	341749.0
23	404579.0
24	494981.0
25	610001.0
26	765071.0
27	959761.0
28	1201948.0
29	1493653.0
30	1879666.0
31	2392280.0
32	3122097.0
33	4290922.0
34	6378153.0
35	1.0609658E7
36	1.9788482E7
37	3.4732663E7
38	3.902588E7
39	9877740.0
40	15921.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.3296745423041	20.40182504239639	8.456292497065986	27.81220791823353
2	27.24905869351358	25.53766629608448	27.81756874114191	19.39570626926003
3	23.87799121175008	26.246369021135802	27.05873806837371	22.816901698740413
4	26.615167645154163	32.307674240051774	20.097417099960584	20.97974101483348
5	27.450760654337646	34.272649123793514	19.281115132531543	18.995475089337297
6	22.87127391309638	36.15152677609055	19.503823233849555	21.47337607696352
7	23.171846707966367	20.725400802667608	32.733567514533554	23.36918497483247
8	22.96352595646067	23.579512033223864	25.53836328164771	27.918598728667753
9	23.635381146035765	22.832074955577948	27.073469486955425	26.459074411430862
10-14	25.6694692929708	27.11615271371998	22.987837029136628	24.226540964172592
15-19	25.284251951701282	26.53917167818682	24.251626039456983	23.92495033065492
20-24	25.055784318981715	26.906128204699176	24.169768304826363	23.868319171492743
25-29	25.034571567231357	26.674432148211075	24.231500791594563	24.05949549296301
30-34	24.842745114947903	26.790235696422894	24.2999878397441	24.067031348885102
35-39	24.735157101008472	26.918483025560125	24.27718132571411	24.06917854771729
40-44	24.85504465660801	26.791256365671558	24.288020255921616	24.065678721798818
45-49	24.717953627702947	26.80133019344964	24.439809077354223	24.040907101493193
50-54	24.57772204261588	26.961955962814375	24.40840495913261	24.051917035437132
55-59	24.748942421763157	26.835768823356233	24.349086011288122	24.066202743592488
60-64	24.5939967178718	26.914591117452215	24.48885416013219	24.002558004543804
65-69	24.5751265779681	27.074917445362296	24.417811548698566	23.932144427971043
70-74	24.715099093392713	27.056053525610796	24.355171541126918	23.873675839869573
75-79	24.570533871140366	27.03479509747328	24.494523849707154	23.900147181679205
80-84	24.690018003848373	27.102284864985755	24.46795170647106	23.739745424694817
85-89	24.826345071015602	27.074842295011702	24.316684608265525	23.782128025707173
90-94	24.79448066075759	27.109388110546533	24.391431462801417	23.704699765894464
95-99	24.963347142790724	27.231378507053034	24.16744332201897	23.637831028137278
100	25.389280521476117	26.94997891174148	23.89894295255982	23.761797614222584
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	71.14188246053762
2	46.64476496277361
3	46.69489291049049
4	47.594908659987645
5	46.44623574367494
6	44.3446499900599
7	46.54103168279884
8	50.882124685128424
9	50.09445555746663
10-14	49.896010257143395
15-19	49.2092022823562
20-24	48.924103490474465
25-29	49.09406706019436
30-34	48.90977646383301
35-39	48.80433564872576
40-44	48.92072337840683
45-49	48.75886072919614
50-54	48.62963907805301
55-59	48.815145165355645
60-64	48.596554722415604
65-69	48.50727100593914
70-74	48.588774933262286
75-79	48.47068105281957
80-84	48.42976342854319
85-89	48.60847309672277
90-94	48.49918042665205
95-99	48.601178170928
100	49.1510781356987
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2076.0
1	1931.5
2	2015.5
3	2554.0
4	3396.0
5	4603.0
6	6032.0
7	7644.0
8	9182.0
9	10525.0
10	11699.5
11	12811.0
12	13816.5
13	14742.0
14	15901.0
15	17435.0
16	19746.5
17	22979.0
18	27913.0
19	35668.0
20	48691.0
21	69697.5
22	98350.0
23	141284.0
24	201566.5
25	281673.5
26	383849.5
27	505413.0
28	649399.0
29	818147.0
30	1002029.5
31	1210593.5
32	1446317.0
33	1702021.5
34	1992339.0
35	2324271.0
36	2683472.0
37	3067004.5
38	3438009.5
39	3784645.0
40	4101713.0
41	4359935.5
42	4608269.0
43	4873804.5
44	5063301.0
45	5214433.0
46	5252760.5
47	5162424.5
48	5111711.5
49	5044285.5
50	4964069.5
51	4887097.0
52	4798693.0
53	4712610.5
54	4581531.5
55	4453397.5
56	4222285.5
57	3864253.0
58	3655705.5
59	3530551.0
60	3301346.0
61	3074150.5
62	2848806.5
63	2575338.0
64	2311990.0
65	2053469.5
66	1818071.0
67	1611219.5
68	1375757.0
69	1136451.0
70	920092.5
71	720414.0
72	570205.5
73	443838.5
74	337529.0
75	249708.0
76	187349.5
77	144194.5
78	105089.0
79	74234.0
80	50555.5
81	33036.5
82	22571.0
83	16827.5
84	12327.5
85	8425.5
86	5703.0
87	4417.5
88	3531.0
89	2937.5
90	2499.5
91	2082.0
92	1750.0
93	1469.0
94	1217.5
95	1030.5
96	867.5
97	685.0
98	540.5
99	446.5
100	403.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	7.801584622180359E-4
5	0.0013424535240826306
6	0.010425628244215134
7	0.025770127927521286
8	0.026688612358217696
9	0.029305739681828553
10-14	0.032005032630715566
15-19	0.06401836481953985
20-24	0.009883390447779904
25-29	0.0
30-34	0.0342903159559468
35-39	0.09766463506625132
40-44	0.13853497901674597
45-49	0.10615480635951705
50-54	0.02647655864854035
55-59	0.06279376502060079
60-64	0.1392107013727628
65-69	0.23860731589033188
70-74	0.27395790039466544
75-79	0.12289183356749499
80-84	0.06671336966340402
85-89	0.18031440123208048
90-94	0.4100808894990539
95-99	0.500622013840606
100	0.344931018257361
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.44586011E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	87.62113243813235
#Duplication Level	Relative count
1	100.0
2	50.679318524908346
3	37.65365538063403
4	29.613255696930487
5	24.96944863776867
6	21.03371432679175
7	19.04967292071023
8	16.986557400618217
9	15.020487384084538
10++	403.8494716411473
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	45166050	3.962344	6.0516043	2
GAAGA	48519365	2.8601081	6.8298783	2
GGAAG	33236030	2.1084003	6.316422	1
GAAAA	36736930	2.0123038	5.6196	2
GGAGA	30862370	1.9578221	5.1631947	1
GGAAA	30366190	1.790019	6.022015	1
GTTTT	18313075	1.5505748	5.3249655	1
GTTTG	15552120	1.270903	5.0560913	1
>>END_MODULE