FastQCFastQC Report
Tue 25 Mar 2014
6492_ACTTGA_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 6492_ACTTGA_R1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 144586011
Filtered Sequences 0
Sequence length 100
%GC 48

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 41566780 3.4011734 4.5948586 9
TCTTC 45338960 2.782374 5.8695335 7
CCAGG 31798110 2.7270186 5.7701554 2
TTCTT 48159820 2.6504748 5.3385744 6
CTCCA 33056370 2.5027292 8.03188 1
CTTCA 36411795 2.472269 5.766959 6
CCAGC 28698460 2.4419334 5.5201416 2
TCCAG 31603950 2.4116352 6.6198463 2
CAGCT 31516530 2.404964 5.2583685 3
GGCAG 27471625 2.374557 10.658869 1
CAGGA 27618875 2.35016 5.1147404 3
CTCCT 33483570 2.2912936 5.8589973 1
GCCAG 25324750 2.1718605 10.993977 1
CTGCT 31471945 2.1706214 5.2670174 3
CCCAG 25382030 2.1597407 6.624692 1
GGCTG 27298085 2.1326556 7.9276214 1
GCTGG 27171390 2.1227574 6.1303697 1
CCACA 25041615 2.0976388 5.31345 2
CCTGG 25359320 1.9656881 5.0439878 1
GGCCA 22871440 1.9614638 7.794882 1
GGGCA 21679395 1.8738958 6.7685413 1
CCAGA 21454045 1.8112947 5.2292204 2
GGGAG 20588775 1.7936608 6.388923 1
GGGGG 19923930 1.7631598 9.062192 1
GGGGC 19609890 1.7217906 6.7036357 1
GCTCC 21628250 1.6633624 5.0045576 1
GCCAC 19399315 1.6506752 7.440298 1
GGGGA 18804685 1.6382338 6.8828325 1
CCCCA 19397825 1.6376338 5.479548 1
GCCTG 19831550 1.5372117 6.704945 1
GGCCT 19600365 1.5192919 5.1829963 1
GCCTC 19451620 1.4959644 5.599126 1
GCCCA 17352865 1.4765441 6.816486 1
GTGGG 18595480 1.4642239 6.345846 1
GTCCA 18824780 1.4364817 8.961891 1
GGGGT 17685810 1.3925956 6.775823 1
GCCAA 15983265 1.3494145 5.2154293 1
GTCTG 18771820 1.3049034 5.884554 1
GTCAG 16846330 1.2956479 6.549535 1
GCCAT 16837010 1.2847989 5.200864 1
GTCCT 17845320 1.2307924 6.8139725 1
GCCCT 14416170 1.1087034 5.0746813 1