##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	6492_ACTTGA_R1.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	144586011	
Filtered Sequences	0	
Sequence length	100	
%GC	48	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.817333746070357	33.0	31.0	34.0	28.0	34.0
2	32.44866624060885	33.0	31.0	34.0	30.0	34.0
3	32.617290921733776	34.0	31.0	34.0	31.0	34.0
4	36.25941210868595	37.0	37.0	37.0	35.0	37.0
5	36.206048626654486	37.0	37.0	37.0	35.0	37.0
6	36.195194699714065	37.0	37.0	37.0	35.0	37.0
7	36.211951424539954	37.0	37.0	37.0	35.0	37.0
8	36.2106913856279	37.0	37.0	37.0	35.0	37.0
9	37.998913733085836	39.0	38.0	39.0	35.0	39.0
10-14	38.18774636780041	39.4	38.2	39.4	35.2	39.4
15-19	39.22459055599784	40.8	39.0	41.0	36.0	41.0
20-24	39.249780171333455	41.0	39.0	41.0	36.0	41.0
25-29	39.06935574147626	40.6	39.0	41.0	35.6	41.0
30-34	38.82995271375181	40.0	38.2	41.0	35.0	41.0
35-39	38.55605711260684	40.0	38.0	41.0	34.4	41.0
40-44	38.42399813907308	40.0	38.0	41.0	34.0	41.0
45-49	38.17335861212742	40.0	37.6	41.0	33.6	41.0
50-54	37.7133295544062	40.0	36.6	41.0	33.0	41.0
55-59	37.16455360539686	39.0	35.4	41.0	32.2	41.0
60-64	36.36119419464446	38.0	35.0	40.0	31.0	41.0
65-69	35.40593792438191	36.6	34.2	39.4	30.0	41.0
70-74	34.40295442966471	35.2	33.8	38.2	29.0	40.0
75-79	32.680121760880446	34.2	31.8	36.0	26.8	38.2
80-84	32.763517296289464	35.0	33.0	35.6	27.6	37.2
85-89	31.984523714400005	34.0	32.0	35.0	26.4	36.2
90-94	31.0874016117645	34.0	31.2	35.0	24.6	35.0
95-99	29.822134532779938	34.0	30.4	35.0	15.8	35.0
100	22.87503608492249	26.0	19.0	31.0	2.0	34.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	196.0
3	570.0
4	907.0
5	2168.0
6	5021.0
7	12388.0
8	28314.0
9	48031.0
10	68991.0
11	92819.0
12	112379.0
13	134109.0
14	158089.0
15	182547.0
16	214314.0
17	247157.0
18	283024.0
19	318197.0
20	351981.0
21	389085.0
22	445888.0
23	535142.0
24	642615.0
25	763764.0
26	936055.0
27	1182623.0
28	1467020.0
29	1845150.0
30	2316668.0
31	2932805.0
32	3818940.0
33	5252742.0
34	7916360.0
35	1.281497E7
36	2.2809871E7
37	3.7860591E7
38	3.4307225E7
39	4087109.0
40	186.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	53.51953823521386	9.434456835038233	4.971470771008174	32.07453415873973
2	27.1641944649235	10.560244500558165	32.72134442661914	29.554216607899185
3	24.715906990476416	14.598313387316564	23.610767572804814	37.075012049402204
4	27.299894178559224	22.275656391129015	22.244790334522747	28.179659095789013
5	28.90183961157902	26.000282973433713	23.772779788495583	21.32509762649168
6	24.34023717550379	28.739168272648453	24.003206644936075	22.917387906911685
7	18.265062309520385	23.099451163363238	40.4704297430268	18.165056784089575
8	20.25846331703556	22.438055919531525	32.96011949593104	24.343361267501876
9	20.343739893342793	20.748135862189322	34.755629298051524	24.152494946416358
10-14	23.68558333636891	26.3156904935235	27.36747973856216	22.631246431545424
15-19	23.981063839068366	24.71876211977013	27.561463062506576	23.73871097865493
20-24	23.783942279173882	24.927178743453958	27.476016611316567	23.812862366055594
25-29	23.74418558288266	24.959645554042183	27.37726068974025	23.918908173334902
30-34	23.71693411765963	24.910781320971857	27.191145125289275	24.181139436079242
35-39	23.715623173792544	24.939292328794068	27.243741825185296	24.101342672228096
40-44	23.753898993185782	24.886945227866416	27.253203840722602	24.105951938225203
45-49	23.744079229561844	24.84301981575238	27.19251387772727	24.220387076958502
50-54	23.700115082364366	24.850800123395064	27.233029618612274	24.2160551756283
55-59	23.666411683492672	24.81465679276538	27.261391836863112	24.257539686878836
60-64	23.675080686608833	24.755177403450176	27.19202916627013	24.377712743670855
65-69	23.69173490373128	24.834850362204207	27.29899034116573	24.174424392898782
70-74	23.812680652718388	24.922744220210717	27.06471149408	24.19986363299089
75-79	23.819528143303025	24.82443751866649	26.992161247355266	24.36387309067522
80-84	23.85175683252072	24.77209948142265	27.07614442061917	24.29999926543746
85-89	23.940039747981707	24.967101887067468	26.83346512066193	24.259393244288894
90-94	24.027259879919658	24.89994204671501	26.640313755724076	24.432484317641258
95-99	24.170231258976962	24.99284971211723	26.576193132159066	24.26072589674674
100	23.981795859905148	25.5392736438382	26.010404284547278	24.468526211709374
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	85.5940723939536
2	56.7184110728227
3	61.790919039878624
4	55.479553274348234
5	50.226937238070704
6	47.25762508241548
7	36.43011909360997
8	44.60182458453744
9	44.496234839759154
10-14	46.316829767914335
15-19	47.71977481772329
20-24	47.596804645229476
25-29	47.66309375621757
30-34	47.898073553738875
35-39	47.81696584602064
40-44	47.85985093141099
45-49	47.96446630652035
50-54	47.91617025799266
55-59	47.92395137037151
60-64	48.05279343027969
65-69	47.86615929663006
70-74	48.01254428570928
75-79	48.183401233978245
80-84	48.151756097958184
85-89	48.1994329922706
90-94	48.45974419756091
95-99	48.4309571557237
100	48.45032207161452
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	606.0
1	404.0
2	178.0
3	161.5
4	187.0
5	222.5
6	238.5
7	267.5
8	348.0
9	487.0
10	631.5
11	761.5
12	1004.0
13	1392.0
14	2050.5
15	3125.0
16	4927.5
17	8257.5
18	13405.5
19	21672.0
20	35261.5
21	55830.5
22	85115.5
23	124562.5
24	181444.5
25	252787.0
26	347412.5
27	476043.0
28	628738.5
29	796811.5
30	989714.5
31	1209035.0
32	1445491.0
33	1698355.5
34	1990998.5
35	2311743.5
36	2642757.5
37	3023054.5
38	3447974.0
39	3840907.5
40	4204958.0
41	4561406.0
42	4874652.0
43	5176833.0
44	5345064.0
45	5459700.0
46	5675230.5
47	5646029.0
48	5494390.5
49	5427698.0
50	5351980.0
51	5307352.5
52	5126640.0
53	4861696.5
54	4710515.0
55	4530900.0
56	4208466.0
57	3816285.0
58	3501681.0
59	3296916.5
60	3056535.0
61	2821323.0
62	2549011.0
63	2266585.5
64	2057354.5
65	1826661.0
66	1584948.0
67	1373512.0
68	1167810.0
69	937225.0
70	715813.0
71	539546.0
72	409601.0
73	303947.0
74	219170.0
75	157522.0
76	116692.0
77	89746.0
78	65520.5
79	42551.0
80	24086.0
81	14190.0
82	8525.5
83	5297.5
84	3252.5
85	2171.5
86	1612.5
87	1146.5
88	729.5
89	488.5
90	330.5
91	222.5
92	136.0
93	87.0
94	56.5
95	35.5
96	23.0
97	16.5
98	12.5
99	17.0
100	27.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.05872628991749416
2	4.5370917660907047E-4
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0043343058963014065
15-19	0.016361887181464602
20-24	0.0
25-29	0.00254284627853797
30-34	0.010720400883042552
35-39	1.1411892399465949E-4
40-44	2.157885108262652E-5
45-49	0.0013818764250989675
50-54	0.0
55-59	0.0
60-64	0.06205911580201213
65-69	0.13194955630942748
70-74	0.1969992795499421
75-79	0.1440633146729527
80-84	0.044692982089394526
85-89	0.15309060570181993
90-94	0.20830424597577424
95-99	0.10607167245246155
100	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.44586011E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	88.43759173819508
#Duplication Level	Relative count
1	100.0
2	58.71764750464209
3	39.72109591117511
4	29.5274546212437
5	22.998218954867557
6	19.663496153700404
7	17.026033574595477
8	15.176778203039145
9	14.00962522263064
10++	441.0511955739134
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AAAAA	41566780	3.4011734	4.5948586	9
TCTTC	45338960	2.782374	5.8695335	7
CCAGG	31798110	2.7270186	5.7701554	2
TTCTT	48159820	2.6504748	5.3385744	6
CTCCA	33056370	2.5027292	8.03188	1
CTTCA	36411795	2.472269	5.766959	6
CCAGC	28698460	2.4419334	5.5201416	2
TCCAG	31603950	2.4116352	6.6198463	2
CAGCT	31516530	2.404964	5.2583685	3
GGCAG	27471625	2.374557	10.658869	1
CAGGA	27618875	2.35016	5.1147404	3
CTCCT	33483570	2.2912936	5.8589973	1
GCCAG	25324750	2.1718605	10.993977	1
CTGCT	31471945	2.1706214	5.2670174	3
CCCAG	25382030	2.1597407	6.624692	1
GGCTG	27298085	2.1326556	7.9276214	1
GCTGG	27171390	2.1227574	6.1303697	1
CCACA	25041615	2.0976388	5.31345	2
CCTGG	25359320	1.9656881	5.0439878	1
GGCCA	22871440	1.9614638	7.794882	1
GGGCA	21679395	1.8738958	6.7685413	1
CCAGA	21454045	1.8112947	5.2292204	2
GGGAG	20588775	1.7936608	6.388923	1
GGGGG	19923930	1.7631598	9.062192	1
GGGGC	19609890	1.7217906	6.7036357	1
GCTCC	21628250	1.6633624	5.0045576	1
GCCAC	19399315	1.6506752	7.440298	1
GGGGA	18804685	1.6382338	6.8828325	1
CCCCA	19397825	1.6376338	5.479548	1
GCCTG	19831550	1.5372117	6.704945	1
GGCCT	19600365	1.5192919	5.1829963	1
GCCTC	19451620	1.4959644	5.599126	1
GCCCA	17352865	1.4765441	6.816486	1
GTGGG	18595480	1.4642239	6.345846	1
GTCCA	18824780	1.4364817	8.961891	1
GGGGT	17685810	1.3925956	6.775823	1
GCCAA	15983265	1.3494145	5.2154293	1
GTCTG	18771820	1.3049034	5.884554	1
GTCAG	16846330	1.2956479	6.549535	1
GCCAT	16837010	1.2847989	5.200864	1
GTCCT	17845320	1.2307924	6.8139725	1
GCCCT	14416170	1.1087034	5.0746813	1
>>END_MODULE