FastQCFastQC Report
Tue 25 Mar 2014
6494_GGCTAC_R2.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 6494_GGCTAC_R2.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 131737035
Filtered Sequences 0
Sequence length 100
%GC 47

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 43712735 3.7649431 5.747354 2
GAAGA 46770735 2.9161208 6.866651 2
GGAAG 30964365 2.165463 6.3811436 1
GAAAA 35773515 1.9885455 5.612055 2
GGAGA 27648135 1.9335457 5.232977 1
GGAAA 28961090 1.8057026 6.1433105 1
GTGGA 22616590 1.766536 5.1828914 1
GTTTT 18023335 1.5589644 5.4554796 1
GTTTG 14668230 1.2741756 5.204695 1