##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	6494_GGCTAC_R2.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	131737035	
Filtered Sequences	0	
Sequence length	100	
%GC	47	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94343087348216	34.0	31.0	34.0	30.0	34.0
2	32.03312688797042	34.0	31.0	34.0	30.0	34.0
3	32.05072004998443	34.0	31.0	34.0	30.0	34.0
4	35.45632523154935	37.0	35.0	37.0	33.0	37.0
5	35.42669054302004	37.0	35.0	37.0	33.0	37.0
6	35.44682564018539	37.0	35.0	37.0	33.0	37.0
7	35.45044850144077	37.0	35.0	37.0	33.0	37.0
8	35.45077041547201	37.0	35.0	37.0	33.0	37.0
9	37.20248475305369	39.0	38.0	39.0	35.0	39.0
10-14	37.421815737692896	39.4	38.2	39.4	34.4	39.4
15-19	38.49635707832653	41.0	39.0	41.0	34.2	41.0
20-24	38.44247589297876	41.0	39.0	41.0	34.2	41.0
25-29	38.23892674220275	40.0	38.0	41.0	34.0	41.0
30-34	37.99844661753622	40.0	38.0	41.0	33.6	41.0
35-39	37.642916882105325	40.0	38.0	41.0	33.0	41.0
40-44	37.53582118498416	40.0	37.8	41.0	32.8	41.0
45-49	37.305513171751585	40.0	37.0	41.0	32.0	41.0
50-54	36.28354800910769	39.0	35.0	40.2	30.6	40.6
55-59	36.522510838353085	39.0	35.0	41.0	31.0	41.0
60-64	35.85687794476322	38.0	35.0	40.4	30.2	41.0
65-69	35.13521552690175	36.8	34.8	39.6	29.6	41.0
70-74	34.56782916208794	35.6	34.6	38.8	30.0	40.8
75-79	33.78019827150353	35.0	34.0	36.8	29.0	39.2
80-84	33.06746092623081	35.0	34.0	35.8	29.0	37.4
85-89	32.49554417707974	35.0	34.0	35.0	28.4	36.4
90-94	31.928450354146804	35.0	33.0	35.0	26.6	35.8
95-99	31.401903986984376	35.0	33.0	35.0	24.8	35.0
100	29.78452738821699	34.0	30.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	1560888.0
3	228142.0
4	146027.0
5	141243.0
6	145708.0
7	145233.0
8	154081.0
9	170412.0
10	182589.0
11	186542.0
12	191097.0
13	191562.0
14	184523.0
15	186958.0
16	193595.0
17	203473.0
18	212792.0
19	223381.0
20	239739.0
21	266193.0
22	305539.0
23	365033.0
24	446635.0
25	552259.0
26	688211.0
27	861250.0
28	1077930.0
29	1342503.0
30	1680663.0
31	2138386.0
32	2783683.0
33	3787602.0
34	5562744.0
35	9126099.0
36	1.6886598E7
37	3.0918186E7
38	3.7725633E7
39	1.0317648E7
40	16255.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.650684866256476	21.253681624153757	8.080280537663535	27.015352971926234
2	26.80771508179154	26.726900298006555	28.355166791176075	18.11021782902583
3	23.583001545465176	27.2062431039229	26.812700771654686	22.39805457895724
4	26.03272255685582	33.10861405594201	20.547149431228316	20.311513955973854
5	26.71109642507941	34.961656924257255	19.779958160396845	18.547288490266485
6	22.46374802655157	36.7415647771234	19.920188306284206	20.874498890040822
7	22.570161172427458	21.0928066700583	33.512929632868534	22.824102524645703
8	22.62340991919786	24.06924630781161	26.094905956077152	27.212437816913376
9	23.275863162075364	23.296111038696328	27.704639522991098	25.72338627623721
10-14	25.276667160319427	27.753229569968763	23.419148953732176	23.550954315979634
15-19	24.8394848932276	27.10967736631472	24.810278909443443	23.240558831014237
20-24	24.60441458534644	27.46967813152046	24.73168197275427	23.194225310378826
25-29	24.602995605265722	27.27103243493235	24.777188977387553	23.34878298241437
30-34	24.4052487177843	27.397241874849783	24.848818914967687	23.34869049239823
35-39	24.278500662282816	27.528420689524953	24.844081061020376	23.348997587171855
40-44	24.42301584447065	27.399249499421586	24.857377229858898	23.320357426248865
45-49	24.23857854524295	27.424585810368374	25.0328525872324	23.30398305715627
50-54	24.11080299883709	27.606552229504857	24.989302494650747	23.29334227700731
55-59	24.27507136233068	27.49273961415047	24.94632832043637	23.28586070308248
60-64	24.135130222994906	27.558394903617256	25.08812463301017	23.218350240377667
65-69	24.104831253226184	27.72431615615128	25.012824722471134	23.1580278681514
70-74	24.26176453720253	27.720685067764432	24.954754400384495	23.06279599464854
75-79	24.128010644127077	27.700229676936374	25.069253089503952	23.102506589432604
80-84	24.238290968281607	27.795304012276105	25.04080509305608	22.925599926386205
85-89	24.399284822730312	27.751531704728215	24.89853380218274	22.950649670358732
90-94	24.382608690299826	27.824782451671158	24.94402794438651	22.8485809136425
95-99	24.60017776691852	27.964095591427572	24.69063482064041	22.7450918210135
100	25.02298451195511	27.731642278047907	24.42795323808932	22.81741997190766
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	70.6660378381827
2	44.91793291081737
3	45.981056124422416
4	46.34423651282968
5	45.2583849153459
6	43.3382469165924
7	45.394263697073164
8	49.83584773611123
9	48.999249438312575
10-14	48.82762147629906
15-19	48.08004372424183
20-24	47.798639895725266
25-29	47.951778587680096
30-34	47.75393921018253
35-39	47.627498249454675
40-44	47.743373270719516
45-49	47.542561602399225
50-54	47.4041452758444
55-59	47.560932065413155
60-64	47.35348046337257
65-69	47.26285912137759
70-74	47.32456053185107
75-79	47.230517233559674
80-84	47.163890894667816
85-89	47.349934493089044
90-94	47.23118960394233
95-99	47.34526958793202
100	47.84040448386277
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	1495.0
1	1502.5
2	1761.5
3	2309.0
4	3180.0
5	4342.0
6	5527.5
7	6760.0
8	7882.5
9	8946.0
10	9778.5
11	10429.5
12	11243.0
13	12107.5
14	13234.0
15	14630.0
16	16789.5
17	19852.5
18	24490.5
19	32199.0
20	45057.5
21	66160.5
22	95402.5
23	140262.0
24	202984.0
25	286089.5
26	397279.0
27	532144.5
28	690398.5
29	875235.5
30	1070715.0
31	1286393.0
32	1530557.0
33	1791866.5
34	2077707.5
35	2399661.0
36	2744964.0
37	3110990.0
38	3467487.0
39	3794292.0
40	4086578.0
41	4322102.0
42	4559735.5
43	4820674.5
44	4980120.5
45	5081216.5
46	5104049.5
47	5018727.5
48	4936558.0
49	4809459.5
50	4661497.5
51	4530257.0
52	4390940.0
53	4239878.0
54	4039169.5
55	3855113.0
56	3595418.5
57	3232784.0
58	3018675.0
59	2842206.0
60	2572057.0
61	2351004.5
62	2137690.5
63	1881396.0
64	1654894.5
65	1451118.0
66	1273743.5
67	1118197.5
68	947198.0
69	775554.5
70	620267.5
71	483890.0
72	382069.5
73	298417.5
74	227307.5
75	167756.0
76	126905.5
77	98560.0
78	70988.5
79	47792.5
80	32227.0
81	21339.5
82	14873.0
83	11411.5
84	8306.0
85	5414.0
86	3512.0
87	2712.0
88	2128.0
89	1741.0
90	1491.0
91	1244.5
92	1041.5
93	876.0
94	724.0
95	624.0
96	509.5
97	415.0
98	344.0
99	287.5
100	311.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	6.0727038527928E-5
5	0.003724085637725185
6	0.005287047791837732
7	0.029237032699270936
8	0.019110039936757344
9	0.026064044936186696
10-14	0.034368467454880855
15-19	0.05899161158439614
20-24	0.016511378140550984
25-29	8.000787326054514E-5
30-34	0.034333397590130973
35-39	0.08375594607848887
40-44	0.10094291252266306
45-49	0.07168447354231101
50-54	0.018991318576435243
55-59	0.052088313662137606
60-64	0.10399323166792086
65-69	0.19330752358287098
70-74	0.2259221941650653
75-79	0.09346149319361864
80-84	0.048496916603595945
85-89	0.1483972976923308
90-94	0.35366273424933237
95-99	0.44412248992851555
100	0.29348466814969687
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.31737035E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	86.8483754032097
#Duplication Level	Relative count
1	100.0
2	48.54424614554165
3	35.06629330994231
4	27.755473836915083
5	23.241456091224993
6	19.83738740258426
7	17.17216018352957
8	15.28963260348841
9	13.670254040012145
10++	374.1641645018724
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	43712735	3.7649431	5.747354	2
GAAGA	46770735	2.9161208	6.866651	2
GGAAG	30964365	2.165463	6.3811436	1
GAAAA	35773515	1.9885455	5.612055	2
GGAGA	27648135	1.9335457	5.232977	1
GGAAA	28961090	1.8057026	6.1433105	1
GTGGA	22616590	1.766536	5.1828914	1
GTTTT	18023335	1.5589644	5.4554796	1
GTTTG	14668230	1.2741756	5.204695	1
>>END_MODULE