FastQCFastQC Report
Tue 25 Mar 2014
6494_GGCTAC_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 6494_GGCTAC_R1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 131737035
Filtered Sequences 0
Sequence length 100
%GC 47

[FAIL] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[WARN] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 41084735 3.3300562 4.409197 9
TCTTC 42831025 2.8338983 5.9311852 7
CCAGG 24556130 2.602913 5.9029937 2
TTCTT 45564045 2.5800562 5.011365 6
CTTCA 33930060 2.48821 5.7084527 6
CTCCA 28788355 2.4668236 8.364095 1
CCAGC 23195945 2.3896372 5.8898244 2
CAGCT 27099850 2.389285 5.535928 3
TCCAG 26764945 2.359758 6.7397556 2
GGCAG 21186040 2.3106241 11.347821 1
CAGGA 22940785 2.3065653 5.1073995 3
CTCCT 29393495 2.2724578 5.8940825 1
CTGCT 27457950 2.1842012 5.4597697 3
GCAGG 19730160 2.1518407 5.097575 2
GCCAG 20182250 2.1392882 11.78663 1
GGCTG 21574035 2.1229231 8.415544 1
GCTGG 21365105 2.102364 6.440885 1
CCCAG 20121255 2.0728838 6.9681864 1
CCACA 21781150 2.06861 5.5726776 2
CCTGG 20152840 1.9273447 5.1297736 1
GGCCA 17718620 1.8781471 8.062006 1
GGGCA 16828870 1.8354161 7.0198064 1
GGGGG 14962795 1.8213533 9.535551 1
CTCTG 22885265 1.8204567 5.1649933 1
CCAGA 18571710 1.8148032 5.4994316 2
GGGAG 16107430 1.8075305 6.7470956 1
GGGGC 14181965 1.6777917 6.882024 1
GCTCC 17765260 1.6512576 5.455988 1
GGGGA 14666750 1.6458615 7.157799 1
GCCAC 15708705 1.6183046 8.099069 1
CCCCA 15742710 1.5762299 5.640662 1
GCCTG 15910400 1.5216131 7.134165 1
GGCCT 15606395 1.4925389 5.4717517 1
GCCTC 15972420 1.4846156 6.209764 1
GTGGG 14455640 1.4635923 6.394421 1
GGGGT 13968700 1.4142909 7.0125785 1
GTCCA 16003885 1.4109983 9.290385 1
GCCCA 13569275 1.3979014 7.1524243 1
GCCAA 14127335 1.3805047 5.572314 1
GGCAT 14935040 1.3548375 5.54831 1
GTCTG 16460980 1.3472865 6.1431975 1
GCCTT 16855925 1.3408402 5.2929497 1
GGCAA 13097980 1.3169273 5.3036137 1
GCCAT 14838665 1.3082654 5.7831726 1
GTCAG 14006695 1.2706226 6.8097825 1
GTCCT 15289750 1.2162557 7.1161427 1
GCCCC 10822905 1.175455 5.378905 1
GCCCT 11604615 1.0786338 5.526143 1
GTCCC 10414100 0.967977 5.217869 1