##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	6491_ATCACG_R2.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	135218638	
Filtered Sequences	0	
Sequence length	100	
%GC	48	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.91141161324225	34.0	31.0	34.0	30.0	34.0
2	32.00436685362857	34.0	31.0	34.0	30.0	34.0
3	32.03541580562289	34.0	31.0	34.0	30.0	34.0
4	35.39962238785455	37.0	37.0	37.0	35.0	37.0
5	35.312452503773926	37.0	35.0	37.0	33.0	37.0
6	35.3364069012439	37.0	36.0	37.0	35.0	37.0
7	35.36694711419886	37.0	36.0	37.0	35.0	37.0
8	35.355973981929914	37.0	36.0	37.0	35.0	37.0
9	37.11901123423533	39.0	38.0	39.0	35.0	39.0
10-14	37.315206641853614	39.4	38.2	39.4	34.8	39.4
15-19	38.44048264707414	41.0	39.0	41.0	35.0	41.0
20-24	38.31468356603326	41.0	39.0	41.0	34.0	41.0
25-29	38.160787321345445	40.6	38.6	41.0	34.0	41.0
30-34	37.92142518696276	40.0	38.0	41.0	33.6	41.0
35-39	37.56939887236551	40.0	38.0	41.0	33.0	41.0
40-44	37.473127744416416	40.0	38.0	41.0	33.0	41.0
45-49	37.210127833117205	40.0	37.0	41.0	32.2	41.0
50-54	36.14984841512751	39.0	35.0	40.2	30.6	40.6
55-59	36.36123176155642	39.0	35.0	41.0	31.0	41.0
60-64	35.6895036407629	37.8	35.0	40.6	30.2	41.0
65-69	34.97516802528362	36.4	35.0	39.6	29.6	41.0
70-74	34.39798775668781	35.4	35.0	38.6	30.0	40.8
75-79	33.65848838530677	35.0	34.0	36.8	29.2	39.2
80-84	32.941883767532104	35.0	34.0	35.8	28.8	37.4
85-89	32.40248905332119	35.0	34.0	35.0	27.6	36.2
90-94	31.897112084504208	35.0	33.2	35.0	26.4	35.8
95-99	31.406787317292753	35.0	33.0	35.0	24.6	35.0
100	29.747583288037557	34.0	30.0	35.0	18.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	2385193.0
3	273744.0
4	170200.0
5	159713.0
6	156114.0
7	149412.0
8	151797.0
9	171701.0
10	183385.0
11	185039.0
12	190274.0
13	193676.0
14	179264.0
15	178857.0
16	183300.0
17	191177.0
18	203070.0
19	214906.0
20	227295.0
21	250821.0
22	284691.0
23	339355.0
24	417004.0
25	504642.0
26	624056.0
27	782777.0
28	982214.0
29	1224054.0
30	1535556.0
31	1962040.0
32	2573361.0
33	3549965.0
34	5317263.0
35	9049631.0
36	1.7697827E7
37	3.2794787E7
38	3.8666976E7
39	1.0888517E7
40	24984.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.97682767981261	17.99922965029851	9.293610179976056	29.730332489912826
2	29.24174402644109	20.55204031858389	29.274445139729927	20.931770515245095
3	22.485959369003556	24.246559117094492	30.78274682814066	22.484734685761293
4	26.062072278772376	32.0560832220284	20.74472479093893	21.1371197082603
5	27.819614113246548	34.449886226067015	19.076264096037747	18.654235564648687
6	22.45304337024728	36.21315940817745	19.713758846961817	21.620038374613458
7	22.74078599409715	20.397425851026416	33.682977474717255	23.178810680159177
8	22.36951893839383	23.131124328592556	26.264667865605297	28.23468886740832
9	23.27690679441137	22.531833644286202	27.3100361169523	26.881223444350123
10-14	25.384172361441102	27.036095316964783	23.560010004277668	24.019722317316443
15-19	25.13770281376391	26.4300994186768	24.47979967818223	23.952398089377066
20-24	24.946565761009925	26.8504950735153	24.40083601131744	23.80210315415733
25-29	24.920744956218808	26.573092638745273	24.499515296847406	24.006647108188513
30-34	24.6709455222426	26.731900702855192	24.598474721723203	23.998679053178996
35-39	24.63909572716439	26.842276150564103	24.51028865732253	24.00833946494898
40-44	24.717035687861657	26.740801027944528	24.55816900386579	23.983994280328027
45-49	24.553370008783904	26.749531292668046	24.717442900021354	23.9796557985267
50-54	24.445044691519836	26.931165541258867	24.669264412025054	23.954525355196242
55-59	24.618794890943853	26.787107290712942	24.592630463239125	24.001467355104083
60-64	24.42857715429835	26.87894470769158	24.780114429281817	23.912363708728254
65-69	24.443217947090616	27.00105690640504	24.6975892053253	23.85813594117905
70-74	24.595179111094247	26.995899265625372	24.583282806228155	23.825638817052234
75-79	24.439014664432456	27.006216718629666	24.74085197821145	23.81391663872643
80-84	24.56914115180692	27.07135638090078	24.69116280450471	23.668339662787584
85-89	24.683515673585323	27.027192592748193	24.57811181888036	23.711179914786122
90-94	24.64084934130325	27.107027855494227	24.60848443435602	23.643638368846503
95-99	24.78311410119783	27.249562807465328	24.421178673117122	23.54614441821972
100	25.227334452955176	26.85743460240215	24.16897906862	23.74625187602268
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	72.70716016972544
2	50.17351454168618
3	44.97069405476485
4	47.19919198703268
5	46.47384967789524
6	44.07308174486074
7	45.91959667425633
8	50.60420780580215
9	50.15813023876149
10-14	49.403894678757545
15-19	49.09010090314097
20-24	48.74866891516726
25-29	48.92739206440732
30-34	48.6696245754216
35-39	48.64743519211336
40-44	48.70102996818969
45-49	48.5330258073106
50-54	48.39957004671608
55-59	48.620262246047936
60-64	48.3409408630266
65-69	48.30135388826967
70-74	48.42081792814648
75-79	48.25293130315889
80-84	48.237480814594505
85-89	48.39469558837145
90-94	48.284487710149754
95-99	48.32925851941755
100	48.97358632897785
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	3042.0
1	3064.5
2	3363.5
3	3835.0
4	4400.5
5	5110.0
6	5811.5
7	6524.5
8	7352.0
9	8479.0
10	9818.0
11	11159.5
12	12528.0
13	14112.5
14	15929.0
15	18216.5
16	21300.5
17	25541.5
18	32023.0
19	42387.0
20	59476.5
21	85799.0
22	121828.5
23	174600.0
24	244781.0
25	335345.0
26	448554.0
27	575734.5
28	721609.5
29	888945.0
30	1059600.5
31	1246938.0
32	1455746.0
33	1682358.5
34	1937141.5
35	2228568.0
36	2543637.0
37	2869230.5
38	3188141.5
39	3496851.5
40	3771930.5
41	4003966.0
42	4229499.5
43	4459851.5
44	4623213.0
45	4731144.0
46	4745867.5
47	4674183.0
48	4651538.5
49	4604395.0
50	4527618.5
51	4479857.0
52	4417941.0
53	4354929.0
54	4254620.5
55	4145699.0
56	3963870.0
57	3658661.0
58	3479300.5
59	3368852.0
60	3157004.5
61	2944962.5
62	2733830.5
63	2473905.0
64	2221224.5
65	1971078.5
66	1729864.0
67	1514583.0
68	1277097.0
69	1046155.0
70	841840.0
71	646801.0
72	497065.0
73	376404.0
74	278897.5
75	201304.0
76	145130.0
77	106842.5
78	75487.0
79	52704.0
80	37156.5
81	26329.0
82	19300.5
83	15210.0
84	12081.0
85	9444.0
86	7542.5
87	6267.5
88	5255.5
89	4453.5
90	3703.5
91	3074.0
92	2563.5
93	2136.0
94	1820.0
95	1553.5
96	1259.5
97	950.5
98	762.0
99	601.0
100	472.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.005411975825403596
2	0.0
3	0.0
4	1.1832688331027266E-5
5	4.0674866137906226E-5
6	0.0015988920107300592
7	0.009457276148573541
8	0.012859913586764572
9	0.012185450351896016
10-14	0.01218249217981326
15-19	0.03548297831545973
20-24	0.003460321793804786
25-29	4.579250384107552E-4
30-34	0.015132973015154908
35-39	0.060150879496360554
40-44	0.0866626093364437
45-49	0.05604005566155754
50-54	0.0029197158456809777
55-59	0.03273897789149452
60-64	0.10655764777042054
65-69	0.1910559105025152
70-74	0.21647001059129142
75-79	0.08225227057826155
80-84	0.02946546466471582
85-89	0.14247030058090068
90-94	0.33312404758876507
95-99	0.412370075787925
100	0.2816542198864627
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
100	1.35218638E8
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Duplicate Percentage	88.79205072771842
#Duplication Level	Relative count
1	100.0
2	56.58770832492635
3	44.64710867196643
4	35.321415600661794
5	29.619466526774545
6	25.53972801743271
7	21.879665873047898
8	19.36160768330576
9	17.90484645494532
10++	456.20838545659984
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
TTTTT	49503135	4.3371134	6.6650314	2
GAAGA	44191025	2.8659708	7.9414134	2
CCTGG	31024650	2.7754025	5.846671	1
GGAAG	30707140	2.1439562	7.3905888	1
GAAAA	34017305	2.0492682	6.2348623	2
GGAGA	28925955	2.019595	5.558608	1
CTTCA	22665640	1.9135994	6.126926	1
GGAAA	28164715	1.8265985	6.8593984	1
GTGGA	23683215	1.8086679	5.017961	1
CCTGA	21450790	1.7824754	5.024982	1
AAGAT	24927735	1.642568	5.0888247	3
GTTTT	18219470	1.5710899	5.721496	1
GTTTG	14945555	1.2684535	5.688326	1
>>END_MODULE