FastQCFastQC Report
Tue 25 Mar 2014
6491_ATCACG_R1.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 6491_ATCACG_R1.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 135218638
Filtered Sequences 0
Sequence length 100
%GC 48

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[OK] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[OK] Overrepresented sequences

No overrepresented sequences

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 41038400 3.4397523 4.5798454 9
CCAGG 30123565 2.787739 5.692462 2
TCTTC 42092110 2.7784522 6.1312575 7
TTCTT 44047750 2.599359 5.5596905 6
CTCCA 31393635 2.5428123 7.953108 1
CCAGC 26916970 2.4647067 5.447944 2
CTTCA 34034645 2.464531 6.198888 6
TCCAG 29833015 2.4421735 6.5753307 2
CAGCT 29594335 2.4226346 5.37533 3
GGCAG 25331175 2.3692327 10.256397 1
CAGGA 25870380 2.3480082 5.1165576 3
CTCCT 31582200 2.3318698 5.775187 1
GCCAG 23779665 2.2006526 11.039944 1
CTGCT 29278065 2.1847963 5.176238 3
GGAGG 23092050 2.182838 5.0357943 1
CCCAG 23656375 2.1661434 6.4722886 1
GCTGG 25336895 2.160205 5.9483094 1
GGCTG 25184815 2.1472387 7.069738 1
CCTGG 23867955 2.0134928 5.6774864 1
GGCCA 21478430 1.9876883 7.3643875 1
TCCAC 23565855 1.9087802 5.1168017 2
GGGCA 20016200 1.8721212 6.422111 1
CTCTG 24657535 1.8400016 5.054084 1
GGGAG 19147735 1.8099909 6.5506816 1
GGGGG 18432310 1.7955368 8.678848 1
GGGGC 18157300 1.7500851 6.089531 1
GCCAC 17948590 1.6434989 7.2705626 1
GGGGA 17330995 1.6382587 6.865631 1
CCCCA 18072440 1.637379 5.2583847 1
GGCCT 18496385 1.560349 5.0157843 1
GCCTG 18434975 1.5551685 6.447894 1
GCCTC 18183420 1.5177625 5.5182734 1
TTCAA 21369990 1.5176388 5.127586 7
GTGGG 17238055 1.4853761 6.221287 1
GCCCA 16196980 1.4831092 6.492145 1
GTCCA 17911145 1.466232 9.660069 1
GGGGT 16185450 1.3946748 6.509046 1
GCCTT 17854290 1.3323281 5.0198956 1
GCCAA 14667630 1.3171952 5.203409 1
GCCAT 16058450 1.3145678 5.4617934 1
GTCAG 15828945 1.309598 7.351038 1
GTCTG 17171460 1.2950377 5.9114585 1
GTCCT 16725370 1.2480854 6.9752707 1
GCCCT 13487790 1.1258203 5.0011444 1