Flowcell BC3UG0ACXX Run Start 2014-03-14 Read Length 100 paired end HiSeq Sequencing Kit Trueseq v3 Sequencer Software HiSeq Control Software 2.0 Gerald version 1.8 Alignment Report: Legend: Total - total reads considered ( random 10% of total reads sequenced ) Total Unique - total reads aligning to only a single location in the reference U0 - # of unique hits with 0 mismatches U1 - # of unique hits with 1 mismatches U2 - # of unique hits with 2 mismatches R012 - # of hits aligning to multiple location in the reference with 0,1 or 2 mismatches NM - # of reads without a match to the reference Mapping Algorithm : GEM allowing up to 2 mismatches Lane 1 Read 1 Sample: CTRL Index: ATCACG Lab ID: 6491 Application: Truseq Stranded mRNA Reference: Homo sapiens (hg19) Total 13521863 Total Unique 7939280 58.7% U0 5756301 42.6% U1 1654057 12.2% U2 528922 3.9% R012 615128 4.5% NM 4553771 33.7% ------------------------------------------------ Lane 1 Read 2 Sample: CTRL Index: ATCACG Lab ID: 6491 Application: Truseq Stranded mRNA Reference: Homo sapiens (hg19) Total 13521863 Total Unique 7537437 55.7% U0 5992243 44.3% U1 1111641 8.2% U2 433553 3.2% R012 575287 4.3% NM 5044818 37.3% ------------------------------------------------ Lane 2 Read 1 Sample: RED Index: ACTTGA Lab ID: 6492 Application: Truseq Stranded mRNA Reference: Homo sapiens (hg19) Total 14458601 Total Unique 8457523 58.5% U0 5882849 40.7% U1 1935108 13.4% U2 639566 4.4% R012 735332 5.1% NM 4723240 32.7% ------------------------------------------------ Lane 2 Read 2 Sample: RED Index: ACTTGA Lab ID: 6492 Application: Truseq Stranded mRNA Reference: Homo sapiens (hg19) Total 14458601 Total Unique 8054614 55.7% U0 6215230 43.0% U1 1316310 9.1% U2 523074 3.6% R012 692568 4.8% NM 5244255 36.3% ------------------------------------------------ Lane 3 Read 1 Sample: SMU1 Index: TAGCTT Lab ID: 6493 Application: Truseq Stranded mRNA Reference: Homo sapiens (hg19) Total 12921962 Total Unique 7029054 54.4% U0 2697065 20.9% U1 3228096 25.0% U2 1103893 8.5% R012 446221 3.5% NM 4284634 33.2% ------------------------------------------------ Lane 3 Read 2 Sample: SMU1 Index: TAGCTT Lab ID: 6493 Application: Truseq Stranded mRNA Reference: Homo sapiens (hg19) Total 12921962 Total Unique 7243311 56.1% U0 5618010 43.5% U1 1161540 9.0% U2 463761 3.6% R012 559569 4.3% NM 4757779 36.8% ------------------------------------------------ Lane 4 Read 1 Sample: MFAP Index: GGCTAC Lab ID: 6494 Application: Truseq Stranded mRNA Reference: Homo sapiens (hg19) Total 13173703 Total Unique 6968602 52.9% U0 2652258 20.1% U1 3186130 24.2% U2 1130214 8.6% R012 486361 3.7% NM 4369005 33.2% ------------------------------------------------ Lane 4 Read 2 Sample: MFAP Index: GGCTAC Lab ID: 6494 Application: Truseq Stranded mRNA Reference: Homo sapiens (hg19) Total 13173703 Total Unique 7341350 55.7% U0 5690348 43.2% U1 1173130 8.9% U2 477872 3.6% R012 639805 4.9% NM 4785252 36.3% ------------------------------------------------