[vast combine]: VAST-TOOLS v2.5.1 [vast combine]: Species assembly: mm10, VASTDB Species key: Mm2 [vast combine]: Using VASTDB -> /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2 [vast combine]: VASTDB Version: vastdb.mm2.23.06.20 [vast combine]: Building Table for COMBI (splice-site based pipeline) [vast combine combi]: Loading strand-unspecific mappability for each EEJ and length: [vast combine combi]: Loading: /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2/FILES/Mm2_COMBI-M-50-gDNA.eff Length: 50 [vast combine combi]: Loading strand-specific mappability for each EEJ and length: [vast combine combi]: Loading: /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2/FILES/Mm2_COMBI-M-50-gDNA-SS.eff Length: 50 [vast combine combi]: Parsing Template file [vast combine combi]: Loading EEJ read counts data [vast combine combi]: SRR5264037_1: found to_combine/SRR5264037_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: SRR5264040_1: found to_combine/SRR5264040_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: SRR5264043_1: found to_combine/SRR5264043_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: SRR5264054_1: found to_combine/SRR5264054_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: SRR5264057_1: found to_combine/SRR5264057_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: SRR5264060_1: found to_combine/SRR5264060_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: SRR5264067_1: found to_combine/SRR5264067_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: SRR5264070_1: found to_combine/SRR5264070_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: SRR5264073_1: found to_combine/SRR5264073_1.info. Sample will be treated as being not strand-specific. [vast combine combi]: Quantifying PSIs [vast combine]: Building Table for EXSK (transcript-based pipeline, single) [vast combine apr]: Loading and parsing data for each sample for EXSK [vast combine apr]: Parsing info and getting Quality scores (Q) for EXSK [vast combine]: Building Table for MULTI (transcript-based pipeline, multiexon) [vast combine apr]: Loading and parsing data for each sample for MULTI [vast combine apr]: Parsing info and getting Quality scores (Q) for MULTI [vast combine]: Building Table for MIC (microexon pipeline) [vast combine]: Building Table for ANNOT (annotation-based pipeline) [vast combine annot]: Loading strand-unspecific mappability for each EEJ and length: [vast combine annot]: Loading: /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2/FILES/Mm2_COMBI-M-50-gDNA.eff Length: 50 [vast combine annot]: Loading strand-unspecific mappability for each EEJ and length: [vast combine annot]: Loading: /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2/FILES/Mm2_COMBI-M-50-gDNA-SS.eff Length: 50 [vast combine annot]: Parsing Template file [vast combine annot]: Loading EEJ read counts data [vast combine annot]: SRR5264037_1: found to_combine/SRR5264037_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: SRR5264040_1: found to_combine/SRR5264040_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: SRR5264043_1: found to_combine/SRR5264043_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: SRR5264054_1: found to_combine/SRR5264054_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: SRR5264057_1: found to_combine/SRR5264057_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: SRR5264060_1: found to_combine/SRR5264060_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: SRR5264067_1: found to_combine/SRR5264067_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: SRR5264070_1: found to_combine/SRR5264070_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: SRR5264073_1: found to_combine/SRR5264073_1.info. Sample will be treated as being not strand-specific. [vast combine annot]: Quantifying PSIs [vast combine]: Building quality score table for intron retention (version 2) [vast combine IR]: SRR5264037_1: found to_combine/SRR5264037_1.info. Sample will be treated as being not strand-specific. [vast combine IR]: SRR5264040_1: found to_combine/SRR5264040_1.info. Sample will be treated as being not strand-specific. [vast combine IR]: SRR5264043_1: found to_combine/SRR5264043_1.info. Sample will be treated as being not strand-specific. [vast combine IR]: SRR5264054_1: found to_combine/SRR5264054_1.info. Sample will be treated as being not strand-specific. [vast combine IR]: SRR5264057_1: found to_combine/SRR5264057_1.info. Sample will be treated as being not strand-specific. [vast combine IR]: SRR5264060_1: found to_combine/SRR5264060_1.info. Sample will be treated as being not strand-specific. [vast combine IR]: SRR5264067_1: found to_combine/SRR5264067_1.info. Sample will be treated as being not strand-specific. [vast combine IR]: SRR5264070_1: found to_combine/SRR5264070_1.info. Sample will be treated as being not strand-specific. [vast combine IR]: SRR5264073_1: found to_combine/SRR5264073_1.info. Sample will be treated as being not strand-specific. [vast combine]: Building Table for intron retention (version 2) Trying to load required package: optparse Merging IR of 9 sample(s)... SRR5264037_1 SRR5264040_1 SRR5264043_1 SRR5264054_1 SRR5264057_1 SRR5264060_1 SRR5264067_1 SRR5264070_1 SRR5264073_1 used (Mb) gc trigger (Mb) max used (Mb) Ncells 1234216 66.0 2164898 115.7 2164898 115.7 Vcells 11619033 88.7 26657121 203.4 26652649 203.4 ... done. [vast combine]: Building Table for Alternative 5'ss choice events [vast combine alt5]: Parsing Template file [vast combine alt5]: Loading mappability files (strand-unspecific and strand-specific): [vast combine alt5]: Loading: /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2/FILES/Mm2_COMBI-M-50-gDNA.eff Length: 50 [vast combine alt5]: Loading: /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2/FILES/Mm2_COMBI-M-50-gDNA-SS.eff Length: 50 [vast combine alt5]: Loading EEJ data for ALT5 [vast combine alt5]: SRR5264037_1: found to_combine/SRR5264037_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: SRR5264040_1: found to_combine/SRR5264040_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: SRR5264043_1: found to_combine/SRR5264043_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: SRR5264054_1: found to_combine/SRR5264054_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: SRR5264057_1: found to_combine/SRR5264057_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: SRR5264060_1: found to_combine/SRR5264060_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: SRR5264067_1: found to_combine/SRR5264067_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: SRR5264070_1: found to_combine/SRR5264070_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: SRR5264073_1: found to_combine/SRR5264073_1.info. Sample will be treated as being not strand-specific. [vast combine alt5]: Starting PSI quantifications [vast combine]: Building Table for Alternative 3'ss choice events [vast combine alt3]: Parsing Template file [vast combine alt3]: Loading mappability files (strand-unspecific and strand-specific): [vast combine alt3]: Loading: /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2/FILES/Mm2_COMBI-M-50-gDNA.eff Length: 50 [vast combine alt3]: Loading: /nfs/users/mirimia/projects/vast-tools/VASTDB/Mm2/FILES/Mm2_COMBI-M-50-gDNA-SS.eff Length: 50 [vast combine alt3]: Loading EEJ data for ALT3 [vast combine alt3]: SRR5264037_1: found to_combine/SRR5264037_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: SRR5264040_1: found to_combine/SRR5264040_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: SRR5264043_1: found to_combine/SRR5264043_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: SRR5264054_1: found to_combine/SRR5264054_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: SRR5264057_1: found to_combine/SRR5264057_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: SRR5264060_1: found to_combine/SRR5264060_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: SRR5264067_1: found to_combine/SRR5264067_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: SRR5264070_1: found to_combine/SRR5264070_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: SRR5264073_1: found to_combine/SRR5264073_1.info. Sample will be treated as being not strand-specific. [vast combine alt3]: Starting PSI quantification [vast combine]: Combining results into a single table [vast combine]: Final table saved as: /nfs/no_backup/jvalcarcel/emancini/anieto/vast_out/INCLUSION_LEVELS_FULL-mm10-9.tab [vast combine]: Combining cRPKMs into a single table [vast combine]: Final cRPKM table saved as: /nfs/no_backup/jvalcarcel/emancini/anieto/vast_out/cRPKM-mm10-9.tab [vast combine]: Final cRPKM and COUNTS table saved as: /nfs/no_backup/jvalcarcel/emancini/anieto/vast_out/cRPKM_AND_COUNTS-mm10-9.tab [vast combine]: Deleting raw_incl/ folder [vast combine]: Deleting raw_reads/ folder [vast combine]: Completed Sat Jan 29 20:34:03 2022