General summary: Acas_rep1_ivt.fastq.gz Acas_rep1_native.fastq.gz Atha_rep1_ivt.fastq.gz Atha_rep1_native.fastq.gz Bnat_rep1_ivt.fastq.gz Bnat_rep1_native.fastq.gz Cfra_rep1_ivt.fastq.gz Cfra_rep1_native.fastq.gz Cowc_rep1_ivt.fastq.gz Cowc_rep1_native.fastq.gz Ddis_rep1_ivt.fastq.gz Ddis_rep1_native.fastq.gz Gthe_rep1_ivt.fastq.gz Gthe_rep1_native.fastq.gz Hsap_rep1_ivt.fastq.gz Hsap_rep1_native.fastq.gz Itha_rep1_ivt.fastq.gz Itha_rep1_native.fastq.gz Mlei_rep1_ivt.fastq.gz Mlei_rep1_native.fastq.gz Mmus_rep1_ivt.fastq.gz Mmus_rep1_native.fastq.gz Mpol_rep1_ivt.fastq.gz Mpol_rep1_native.fastq.gz Ngru_rep1_ivt.fastq.gz Ngru_rep1_native.fastq.gz Nvec_rep1_ivt.fastq.gz Nvec_rep1_native.fastq.gz Scer_rep1_ivt.fastq.gz Scer_rep1_native.fastq.gz Scin_rep1_ivt.fastq.gz Scin_rep1_native.fastq.gz Skew_rep1_ivt.fastq.gz Skew_rep1_native.fastq.gz Smic_rep1_ivt.fastq.gz Smic_rep1_native.fastq.gz Spun_rep1_ivt.fastq.gz Spun_rep1_native.fastq.gz Tthe_rep1_ivt.fastq.gz Tthe_rep1_native.fastq.gz Mean read length: 285.4 448.7 283.5 389.7 227.8 260.9 268.9 315.9 465.3 937.7 263.9 682.6 244.7 305.4 256.2 557.9 268.9 311.8 370.1 542.5 235.3 663.4 299.1 569.6 383.9 521.5 225.3 255.0 220.1 214.8 362.3 574.5 300.9 350.9 323.3 335.3 290.7 570.8 302.1 126.0 Mean read quality: 13.8 10.1 10.3 11.3 13.3 10.0 13.3 12.9 14.1 14.6 13.9 15.8 13.3 9.8 12.7 11.2 9.9 10.4 11.6 10.8 12.7 12.3 13.1 11.6 13.7 12.8 12.3 10.3 13.9 10.5 10.2 11.0 12.3 11.6 12.3 12.0 11.2 12.5 11.0 7.7 Median read length: 248.0 157.0 260.0 147.0 210.0 138.0 244.0 169.0 362.0 869.0 216.0 163.0 200.0 155.0 258.0 189.0 190.0 150.0 322.0 196.0 239.0 246.0 257.0 182.0 300.0 168.0 224.0 122.0 203.0 96.0 309.0 192.0 271.0 187.0 280.0 189.0 242.0 207.0 281.0 89.0 Median read quality: 14.4 10.5 9.7 11.0 14.2 9.9 13.9 13.4 14.6 14.7 14.6 16.5 13.7 9.5 13.3 11.7 9.4 10.0 11.5 11.0 13.2 12.6 13.7 11.9 14.3 13.4 12.9 10.4 14.5 9.4 10.2 11.5 12.6 11.8 12.4 12.1 11.3 13.2 10.8 7.5 Number of reads: 6,715.0 4,256.0 3,546.0 3,229.0 1,962.0 1,992.0 2,178.0 4,036.0 1,930.0 6,150.0 7,251.0 9,529.0 6,148.0 2,240.0 5,974.0 4,382.0 2,654.0 2,851.0 4,548.0 4,202.0 7,375.0 5,173.0 4,043.0 2,372.0 6,750.0 7,885.0 1,550.0 7,365.0 4,853.0 5,331.0 2,646.0 4,221.0 3,439.0 1,457.0 3,700.0 2,561.0 2,413.0 2,616.0 3,033.0 8,025.0 Read length N50: 302.0 1,627.0 316.0 1,107.0 273.0 472.0 316.0 628.0 644.0 1,791.0 288.0 1,888.0 296.0 821.0 292.0 1,620.0 359.0 761.0 396.0 1,839.0 282.0 1,512.0 355.0 1,482.0 507.0 1,347.0 276.0 576.0 232.0 544.0 433.0 1,831.0 351.0 654.0 402.0 566.0 364.0 1,612.0 339.0 137.0 STDEV read length: 208.9 655.4 201.4 558.2 176.5 365.8 203.7 440.9 341.1 744.7 241.0 1,035.6 198.0 456.6 156.7 809.3 350.8 453.4 256.3 798.9 137.8 809.9 218.4 728.3 311.0 781.3 133.7 378.7 155.2 453.8 269.5 826.1 224.1 434.6 261.2 434.4 222.2 799.6 225.4 157.8 Total bases: 1,916,785.0 1,909,866.0 1,005,270.0 1,258,203.0 446,911.0 519,691.0 585,573.0 1,274,976.0 898,117.0 5,766,862.0 1,913,415.0 6,504,874.0 1,504,488.0 684,021.0 1,530,836.0 2,444,786.0 713,779.0 888,960.0 1,683,241.0 2,279,767.0 1,735,534.0 3,431,722.0 1,209,164.0 1,350,990.0 2,591,296.0 4,112,305.0 349,245.0 1,877,929.0 1,067,974.0 1,145,134.0 958,707.0 2,424,977.0 1,034,898.0 511,319.0 1,196,394.0 858,729.0 701,396.0 1,493,201.0 916,218.0 1,011,358.0 Number, percentage and megabases of reads above quality cutoffs >Q5: 6614 (98.5%) 1.9Mb 4066 (95.5%) 1.9Mb 3479 (98.1%) 1.0Mb 3153 (97.6%) 1.2Mb 1902 (96.9%) 0.4Mb 1852 (93.0%) 0.5Mb 2115 (97.1%) 0.6Mb 3951 (97.9%) 1.3Mb 1906 (98.8%) 0.9Mb 6131 (99.7%) 5.8Mb 7152 (98.6%) 1.9Mb 9428 (98.9%) 6.5Mb 6081 (98.9%) 1.5Mb 2100 (93.8%) 0.7Mb 5863 (98.1%) 1.5Mb 4179 (95.4%) 2.4Mb 2313 (87.2%) 0.7Mb 2754 (96.6%) 0.9Mb 4478 (98.5%) 1.7Mb 4078 (97.0%) 2.3Mb 7211 (97.8%) 1.7Mb 5097 (98.5%) 3.4Mb 3981 (98.5%) 1.2Mb 2329 (98.2%) 1.3Mb 6606 (97.9%) 2.6Mb 7831 (99.3%) 4.1Mb 1496 (96.5%) 0.3Mb 7025 (95.4%) 1.9Mb 4741 (97.7%) 1.1Mb 5087 (95.4%) 1.1Mb 2590 (97.9%) 1.0Mb 3970 (94.1%) 2.4Mb 3373 (98.1%) 1.0Mb 1402 (96.2%) 0.5Mb 3637 (98.3%) 1.2Mb 2511 (98.0%) 0.9Mb 2351 (97.4%) 0.7Mb 2528 (96.6%) 1.5Mb 2945 (97.1%) 0.9Mb 7483 (93.2%) 1.0Mb >Q7: 6396 (95.2%) 1.9Mb 3389 (79.6%) 1.8Mb 3057 (86.2%) 0.9Mb 2866 (88.8%) 1.2Mb 1823 (92.9%) 0.4Mb 1498 (75.2%) 0.5Mb 2023 (92.9%) 0.6Mb 3789 (93.9%) 1.3Mb 1856 (96.2%) 0.9Mb 6103 (99.2%) 5.8Mb 6882 (94.9%) 1.9Mb 9046 (94.9%) 6.4Mb 5862 (95.3%) 1.5Mb 1809 (80.8%) 0.6Mb 5590 (93.6%) 1.5Mb 3756 (85.7%) 2.4Mb 1856 (69.9%) 0.6Mb 2368 (83.1%) 0.8Mb 4263 (93.7%) 1.6Mb 3689 (87.8%) 2.2Mb 6932 (94.0%) 1.7Mb 4912 (95.0%) 3.4Mb 3833 (94.8%) 1.2Mb 2193 (92.5%) 1.3Mb 6342 (94.0%) 2.6Mb 7505 (95.2%) 4.1Mb 1425 (91.9%) 0.3Mb 6085 (82.6%) 1.8Mb 4614 (95.1%) 1.0Mb 4195 (78.7%) 1.0Mb 2402 (90.8%) 0.9Mb 3487 (82.6%) 2.4Mb 3227 (93.8%) 1.0Mb 1327 (91.1%) 0.5Mb 3492 (94.4%) 1.2Mb 2333 (91.1%) 0.8Mb 2138 (88.6%) 0.7Mb 2384 (91.1%) 1.5Mb 2706 (89.2%) 0.9Mb 4931 (61.4%) 0.7Mb >Q10: 5736 (85.4%) 1.7Mb 2338 (54.9%) 1.5Mb 1630 (46.0%) 0.5Mb 2029 (62.8%) 1.0Mb 1508 (76.9%) 0.4Mb 979 (49.1%) 0.4Mb 1754 (80.5%) 0.5Mb 3337 (82.7%) 1.2Mb 1707 (88.4%) 0.8Mb 5888 (95.7%) 5.6Mb 6188 (85.3%) 1.7Mb 8292 (87.0%) 6.2Mb 5154 (83.8%) 1.3Mb 1018 (45.4%) 0.5Mb 4725 (79.1%) 1.3Mb 2913 (66.5%) 2.0Mb 1228 (46.3%) 0.4Mb 1417 (49.7%) 0.6Mb 3261 (71.7%) 1.4Mb 2553 (60.8%) 2.0Mb 5879 (79.7%) 1.5Mb 4233 (81.8%) 3.1Mb 3192 (79.0%) 1.0Mb 1659 (69.9%) 1.2Mb 5665 (83.9%) 2.4Mb 6453 (81.8%) 3.9Mb 1140 (73.5%) 0.3Mb 3992 (54.2%) 1.4Mb 4199 (86.5%) 1.0Mb 2428 (45.5%) 0.9Mb 1410 (53.3%) 0.7Mb 2541 (60.2%) 2.1Mb 2565 (74.6%) 0.8Mb 1059 (72.7%) 0.4Mb 2678 (72.4%) 1.0Mb 1872 (73.1%) 0.7Mb 1550 (64.2%) 0.5Mb 1967 (75.2%) 1.4Mb 1795 (59.2%) 0.6Mb 995 (12.4%) 0.2Mb >Q12: 4961 (73.9%) 1.4Mb 1342 (31.5%) 0.9Mb 980 (27.6%) 0.3Mb 1318 (40.8%) 0.8Mb 1312 (66.9%) 0.3Mb 640 (32.1%) 0.2Mb 1485 (68.2%) 0.4Mb 2685 (66.5%) 1.1Mb 1462 (75.8%) 0.7Mb 5374 (87.4%) 5.0Mb 5481 (75.6%) 1.5Mb 7932 (83.2%) 6.0Mb 4394 (71.5%) 1.1Mb 575 (25.7%) 0.3Mb 3737 (62.6%) 1.0Mb 2000 (45.6%) 1.3Mb 894 (33.7%) 0.3Mb 916 (32.1%) 0.5Mb 1960 (43.1%) 0.8Mb 1682 (40.0%) 1.6Mb 4651 (63.1%) 1.2Mb 3117 (60.3%) 2.4Mb 2615 (64.7%) 0.8Mb 1159 (48.9%) 0.8Mb 4816 (71.3%) 2.1Mb 5386 (68.3%) 3.6Mb 901 (58.1%) 0.2Mb 2417 (32.8%) 1.0Mb 3774 (77.8%) 0.9Mb 1936 (36.3%) 0.8Mb 588 (22.2%) 0.3Mb 1956 (46.3%) 1.8Mb 1929 (56.1%) 0.6Mb 679 (46.6%) 0.2Mb 2013 (54.4%) 0.8Mb 1322 (51.6%) 0.4Mb 1022 (42.4%) 0.3Mb 1627 (62.2%) 1.2Mb 1144 (37.7%) 0.4Mb 329 (4.1%) 0.1Mb >Q15: 2798 (41.7%) 0.9Mb 169 (4.0%) 0.1Mb 382 (10.8%) 0.1Mb 503 (15.6%) 0.4Mb 778 (39.7%) 0.2Mb 204 (10.2%) 0.1Mb 808 (37.1%) 0.2Mb 1119 (27.7%) 0.6Mb 863 (44.7%) 0.4Mb 2746 (44.7%) 2.4Mb 3212 (44.3%) 0.9Mb 6560 (68.8%) 5.0Mb 1946 (31.7%) 0.6Mb 129 (5.8%) 0.1Mb 1673 (28.0%) 0.5Mb 502 (11.5%) 0.2Mb 401 (15.1%) 0.1Mb 356 (12.5%) 0.2Mb 626 (13.8%) 0.2Mb 236 (5.6%) 0.1Mb 2059 (27.9%) 0.5Mb 653 (12.6%) 0.4Mb 1408 (34.8%) 0.5Mb 265 (11.2%) 0.1Mb 2877 (42.6%) 1.3Mb 1865 (23.7%) 1.7Mb 408 (26.3%) 0.1Mb 388 (5.3%) 0.1Mb 2048 (42.2%) 0.5Mb 940 (17.6%) 0.6Mb 90 (3.4%) 0.0Mb 638 (15.1%) 0.6Mb 821 (23.9%) 0.3Mb 214 (14.7%) 0.1Mb 941 (25.4%) 0.4Mb 492 (19.2%) 0.2Mb 349 (14.5%) 0.1Mb 694 (26.5%) 0.6Mb 375 (12.4%) 0.1Mb 57 (0.7%) 0.0Mb Top 5 highest mean basecall quality scores and their read lengths 1: 23.9 (385) 18.5 (2769) 22.8 (500) 21.1 (1685) 22.0 (356) 20.5 (329) 22.6 (316) 21.9 (1038) 23.1 (1518) 26.1 (1507) 24.6 (879) 36.5 (131) 22.2 (580) 21.4 (1548) 22.4 (264) 22.6 (327) 24.6 (160) 20.6 (164) 20.5 (222) 21.2 (320) 26.3 (94) 24.2 (1504) 23.3 (622) 21.7 (118) 23.3 (203) 23.4 (159) 20.3 (180) 20.9 (331) 23.4 (467) 26.3 (1562) 19.6 (166) 22.5 (136) 22.0 (294) 21.2 (573) 26.1 (705) 23.7 (207) 21.3 (568) 22.8 (327) 22.2 (251) 20.5 (122) 2: 23.7 (745) 18.4 (353) 21.8 (164) 20.9 (298) 21.8 (637) 20.1 (705) 22.4 (277) 21.8 (340) 22.4 (1683) 24.6 (1502) 24.4 (452) 32.8 (125) 21.7 (531) 21.0 (1697) 22.0 (1082) 21.6 (341) 24.5 (1429) 20.6 (388) 20.4 (510) 19.7 (329) 23.3 (119) 23.8 (502) 23.2 (1497) 21.4 (149) 22.9 (181) 22.4 (2741) 20.0 (319) 20.7 (291) 23.3 (952) 22.8 (1510) 19.5 (177) 21.3 (299) 21.8 (439) 20.9 (160) 25.1 (194) 22.8 (159) 21.0 (276) 22.3 (1258) 21.3 (242) 20.0 (117) 3: 22.6 (1307) 18.1 (407) 21.2 (565) 20.4 (123) 21.6 (280) 19.7 (140) 21.9 (393) 21.7 (1149) 22.1 (685) 23.9 (472) 22.8 (311) 31.2 (125) 21.6 (325) 20.8 (346) 21.9 (489) 21.6 (132) 24.4 (157) 20.5 (551) 20.4 (1860) 19.6 (308) 21.3 (173) 22.7 (1167) 22.8 (350) 20.6 (1171) 22.4 (1096) 22.1 (1833) 19.8 (231) 20.6 (328) 23.3 (487) 22.7 (1478) 19.2 (281) 20.5 (912) 20.9 (467) 20.5 (381) 22.1 (382) 21.5 (157) 20.8 (447) 21.8 (1383) 20.7 (213) 19.8 (623) 4: 22.6 (330) 18.1 (151) 20.9 (207) 20.1 (1075) 21.5 (1159) 19.4 (282) 21.6 (436) 21.4 (670) 22.1 (545) 23.8 (978) 22.7 (1168) 30.9 (126) 21.5 (361) 20.2 (158) 20.8 (1054) 21.3 (497) 23.5 (156) 20.2 (154) 20.2 (257) 18.8 (323) 21.2 (280) 22.4 (1631) 22.6 (361) 20.2 (322) 22.2 (250) 22.0 (2771) 19.8 (237) 20.2 (322) 23.2 (648) 22.2 (1556) 19.2 (501) 20.3 (1139) 20.6 (420) 20.1 (121) 21.8 (1441) 21.3 (755) 20.8 (184) 20.9 (1212) 20.6 (314) 19.1 (156) 5: 22.5 (939) 17.8 (112) 20.7 (839) 20.1 (1105) 21.4 (198) 19.3 (159) 21.3 (172) 21.2 (723) 21.7 (490) 23.8 (991) 22.4 (548) 29.4 (95) 21.5 (1244) 20.0 (1549) 20.7 (787) 21.1 (207) 22.9 (158) 20.1 (138) 20.2 (306) 18.2 (293) 21.1 (160) 22.3 (1264) 22.5 (726) 20.0 (1331) 22.1 (621) 21.9 (3534) 19.5 (314) 19.9 (336) 22.7 (562) 22.1 (1932) 19.2 (253) 20.2 (1069) 20.4 (314) 20.1 (732) 21.8 (553) 20.8 (759) 20.7 (937) 20.9 (596) 20.6 (348) 19.1 (137) Top 5 longest reads and their mean basecall quality score 1: 2808 (12.9) 5830 (7.9) 2332 (5.2) 3884 (8.7) 3419 (8.5) 3620 (13.4) 2502 (9.7) 3639 (16.0) 2087 (20.1) 6005 (7.0) 5264 (7.5) 8464 (23.1) 6591 (6.0) 3392 (8.5) 3638 (7.6) 7907 (10.4) 7259 (8.3) 3685 (14.4) 2599 (13.8) 4824 (7.2) 2678 (12.5) 6053 (18.7) 3535 (5.7) 4046 (14.0) 5770 (6.9) 6504 (7.3) 2232 (12.7) 5495 (14.3) 2708 (7.5) 5378 (9.4) 2697 (11.3) 6434 (15.7) 3389 (12.5) 3631 (13.2) 3125 (16.1) 7907 (8.7) 2018 (16.9) 4548 (8.0) 2107 (12.5) 5017 (9.1) 2: 2477 (14.4) 4753 (7.8) 1865 (10.9) 3866 (10.2) 2116 (8.7) 2388 (13.0) 2484 (7.3) 3620 (15.5) 2055 (11.1) 4832 (16.7) 3016 (14.8) 7841 (8.9) 5018 (7.6) 3198 (14.8) 2568 (15.4) 5976 (8.1) 4322 (8.6) 3565 (9.4) 2559 (14.2) 4636 (15.6) 2165 (10.1) 6007 (17.5) 2478 (4.5) 3792 (12.6) 3078 (14.2) 4529 (8.7) 1125 (12.7) 3765 (14.2) 2669 (15.0) 5000 (8.3) 2618 (15.1) 4282 (6.7) 2418 (9.9) 3383 (13.1) 2878 (7.4) 3614 (15.8) 1874 (14.8) 4487 (9.2) 2037 (13.0) 2287 (9.9) 3: 2227 (8.1) 4439 (15.2) 1846 (12.5) 3818 (10.8) 1548 (13.3) 2124 (11.6) 2241 (13.9) 3618 (14.7) 2029 (13.3) 4689 (8.7) 2596 (17.3) 7807 (8.6) 2228 (5.3) 3197 (12.0) 2107 (16.7) 5435 (11.1) 3564 (14.4) 3541 (16.1) 2259 (13.5) 3818 (12.6) 1958 (11.8) 5966 (15.0) 2120 (14.9) 3636 (14.6) 2960 (16.4) 4131 (6.5) 1038 (16.7) 3699 (14.5) 2654 (12.7) 3529 (15.0) 2382 (16.5) 3877 (12.5) 2286 (14.5) 3096 (13.8) 2276 (13.7) 3451 (10.0) 1527 (8.5) 3986 (13.5) 1987 (15.3) 2029 (12.7) 4: 2166 (11.8) 4358 (12.4) 1782 (9.6) 3809 (13.6) 1536 (11.2) 2043 (12.2) 1983 (14.9) 3618 (15.2) 1990 (13.2) 4657 (16.8) 2485 (15.6) 7797 (8.1) 2022 (12.3) 3050 (11.5) 1982 (11.4) 5255 (13.5) 3442 (14.7) 3511 (12.4) 2172 (14.1) 3797 (12.4) 1831 (13.9) 4985 (11.1) 1906 (8.2) 3629 (10.1) 2898 (12.5) 3829 (16.5) 945 (16.6) 3686 (13.8) 1940 (20.0) 3527 (13.8) 1962 (14.5) 3784 (13.0) 2027 (15.2) 2896 (10.2) 1957 (7.0) 3450 (10.8) 1491 (13.2) 3743 (15.1) 1963 (15.1) 1993 (13.7) 5: 2022 (20.5) 4316 (13.0) 1696 (12.5) 3649 (13.1) 1438 (19.3) 2022 (14.7) 1495 (12.8) 3599 (18.3) 1932 (13.9) 3937 (14.7) 2253 (15.8) 7400 (9.0) 1994 (13.5) 2809 (12.5) 1952 (18.1) 5203 (13.3) 3002 (9.6) 3504 (18.9) 2088 (10.9) 3791 (11.1) 1828 (15.1) 4968 (12.0) 1902 (12.8) 3627 (14.2) 2654 (15.0) 3765 (17.5) 848 (14.8) 3347 (14.5) 1785 (21.4) 3478 (17.9) 1894 (14.6) 3739 (11.6) 2008 (15.5) 2857 (11.3) 1919 (16.4) 3251 (12.2) 1482 (14.3) 3670 (12.5) 1960 (14.3) 1971 (10.9)