2026-07-01 09:58:22,904 NanoComp 1.18.0 started with arguments Namespace(threads=4, outdir='../report/nanocomp_fastq/', prefix='', verbose=False, raw=False, store=False, tsv_stats=False, no_static=False, readtype='1D', maxlength=None, minlength=None, barcoded=False, split_runs=False, format='png', names=None, colors=None, plot='violin', title=None, dpi=100, fasta=None, fastq=['Acas_rep1_ivt.fastq.gz', 'Acas_rep1_native.fastq.gz', 'Atha_rep1_ivt.fastq.gz', 'Atha_rep1_native.fastq.gz', 'Bnat_rep1_ivt.fastq.gz', 'Bnat_rep1_native.fastq.gz', 'Cfra_rep1_ivt.fastq.gz', 'Cfra_rep1_native.fastq.gz', 'Cowc_rep1_ivt.fastq.gz', 'Cowc_rep1_native.fastq.gz', 'Ddis_rep1_ivt.fastq.gz', 'Ddis_rep1_native.fastq.gz', 'Gthe_rep1_ivt.fastq.gz', 'Gthe_rep1_native.fastq.gz', 'Hsap_rep1_ivt.fastq.gz', 'Hsap_rep1_native.fastq.gz', 'Itha_rep1_ivt.fastq.gz', 'Itha_rep1_native.fastq.gz', 'Mlei_rep1_ivt.fastq.gz', 'Mlei_rep1_native.fastq.gz', 'Mmus_rep1_ivt.fastq.gz', 'Mmus_rep1_native.fastq.gz', 'Mpol_rep1_ivt.fastq.gz', 'Mpol_rep1_native.fastq.gz', 'Ngru_rep1_ivt.fastq.gz', 'Ngru_rep1_native.fastq.gz', 'Nvec_rep1_ivt.fastq.gz', 'Nvec_rep1_native.fastq.gz', 'Scer_rep1_ivt.fastq.gz', 'Scer_rep1_native.fastq.gz', 'Scin_rep1_ivt.fastq.gz', 'Scin_rep1_native.fastq.gz', 'Skew_rep1_ivt.fastq.gz', 'Skew_rep1_native.fastq.gz', 'Smic_rep1_ivt.fastq.gz', 'Smic_rep1_native.fastq.gz', 'Spun_rep1_ivt.fastq.gz', 'Spun_rep1_native.fastq.gz', 'Tthe_rep1_ivt.fastq.gz', 'Tthe_rep1_native.fastq.gz'], fastq_rich=None, summary=None, bam=None, ubam=None, cram=None, pickle=None, feather=None, path='../report/nanocomp_fastq/')
2026-07-01 09:58:22,904 Python version is: 3.9.23 | packaged by conda-forge | (main, Jun  4 2025, 17:57:12)  [GCC 13.3.0]
2026-07-01 09:58:22,926 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:22,926 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:22,927 Nanoget: Decompressing gzipped fastq Acas_rep1_ivt.fastq.gz
2026-07-01 09:58:22,927 Nanoget: Decompressing gzipped fastq Acas_rep1_native.fastq.gz
2026-07-01 09:58:22,927 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:22,928 Nanoget: Decompressing gzipped fastq Atha_rep1_ivt.fastq.gz
2026-07-01 09:58:22,929 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:22,930 Nanoget: Decompressing gzipped fastq Atha_rep1_native.fastq.gz
2026-07-01 09:58:23,510 Reduced DataFrame memory usage from 0.054229736328125Mb to 0.020412445068359375Mb
2026-07-01 09:58:23,519 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:23,523 Nanoget: Decompressing gzipped fastq Bnat_rep1_ivt.fastq.gz
2026-07-01 09:58:23,585 Reduced DataFrame memory usage from 0.0493927001953125Mb to 0.018598556518554688Mb
2026-07-01 09:58:23,592 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:23,596 Nanoget: Decompressing gzipped fastq Bnat_rep1_native.fastq.gz
2026-07-01 09:58:23,756 Reduced DataFrame memory usage from 0.030059814453125Mb to 0.011348724365234375Mb
2026-07-01 09:58:23,778 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:23,783 Nanoget: Decompressing gzipped fastq Cfra_rep1_ivt.fastq.gz
2026-07-01 09:58:23,849 Reduced DataFrame memory usage from 0.1025848388671875Mb to 0.03854560852050781Mb
2026-07-01 09:58:23,853 Reduced DataFrame memory usage from 0.0650634765625Mb to 0.02447509765625Mb
2026-07-01 09:58:23,863 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:23,865 Reduced DataFrame memory usage from 0.030517578125Mb to 0.0115203857421875Mb
2026-07-01 09:58:23,866 Nanoget: Decompressing gzipped fastq Cfra_rep1_native.fastq.gz
2026-07-01 09:58:23,866 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:23,866 Nanoget: Decompressing gzipped fastq Cowc_rep1_ivt.fastq.gz
2026-07-01 09:58:23,866 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:23,867 Nanoget: Decompressing gzipped fastq Cowc_rep1_native.fastq.gz
2026-07-01 09:58:24,023 Reduced DataFrame memory usage from 0.033355712890625Mb to 0.012584686279296875Mb
2026-07-01 09:58:24,045 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:24,049 Nanoget: Decompressing gzipped fastq Ddis_rep1_ivt.fastq.gz
2026-07-01 09:58:24,304 Reduced DataFrame memory usage from 0.029571533203125Mb to 0.011165618896484375Mb
2026-07-01 09:58:24,312 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:24,316 Nanoget: Decompressing gzipped fastq Ddis_rep1_native.fastq.gz
2026-07-01 09:58:24,522 Reduced DataFrame memory usage from 0.06170654296875Mb to 0.02321624755859375Mb
2026-07-01 09:58:24,523 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:24,529 Nanoget: Decompressing gzipped fastq Gthe_rep1_ivt.fastq.gz
2026-07-01 09:58:25,039 Reduced DataFrame memory usage from 0.1107635498046875Mb to 0.04161262512207031Mb
2026-07-01 09:58:25,040 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:25,050 Nanoget: Decompressing gzipped fastq Gthe_rep1_native.fastq.gz
2026-07-01 09:58:25,271 Reduced DataFrame memory usage from 0.09393310546875Mb to 0.03530120849609375Mb
2026-07-01 09:58:25,292 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:25,297 Nanoget: Decompressing gzipped fastq Hsap_rep1_ivt.fastq.gz
2026-07-01 09:58:25,380 Reduced DataFrame memory usage from 0.0343017578125Mb to 0.012939453125Mb
2026-07-01 09:58:25,398 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:25,425 Nanoget: Decompressing gzipped fastq Hsap_rep1_native.fastq.gz
2026-07-01 09:58:26,061 Reduced DataFrame memory usage from 0.091278076171875Mb to 0.034305572509765625Mb
2026-07-01 09:58:26,068 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:26,074 Nanoget: Decompressing gzipped fastq Itha_rep1_ivt.fastq.gz
2026-07-01 09:58:26,316 Reduced DataFrame memory usage from 0.093963623046875Mb to 0.035312652587890625Mb
2026-07-01 09:58:26,330 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:26,336 Nanoget: Decompressing gzipped fastq Itha_rep1_native.fastq.gz
2026-07-01 09:58:26,470 Reduced DataFrame memory usage from 0.040618896484375Mb to 0.015308380126953125Mb
2026-07-01 09:58:26,477 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:26,483 Nanoget: Decompressing gzipped fastq Mlei_rep1_ivt.fastq.gz
2026-07-01 09:58:26,505 Reduced DataFrame memory usage from 0.066986083984375Mb to 0.025196075439453125Mb
2026-07-01 09:58:26,536 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:26,542 Nanoget: Decompressing gzipped fastq Mlei_rep1_native.fastq.gz
2026-07-01 09:58:26,772 Reduced DataFrame memory usage from 0.0436248779296875Mb to 0.016435623168945312Mb
2026-07-01 09:58:26,783 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:26,787 Nanoget: Decompressing gzipped fastq Mmus_rep1_ivt.fastq.gz
2026-07-01 09:58:27,037 Reduced DataFrame memory usage from 0.1455230712890625Mb to 0.05464744567871094Mb
2026-07-01 09:58:27,058 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:27,059 Nanoget: Decompressing gzipped fastq Mmus_rep1_native.fastq.gz
2026-07-01 09:58:27,272 Reduced DataFrame memory usage from 0.06951904296875Mb to 0.02614593505859375Mb
2026-07-01 09:58:27,279 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:27,285 Nanoget: Decompressing gzipped fastq Mpol_rep1_ivt.fastq.gz
2026-07-01 09:58:27,572 Reduced DataFrame memory usage from 0.064239501953125Mb to 0.024166107177734375Mb
2026-07-01 09:58:27,581 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:27,581 Nanoget: Decompressing gzipped fastq Mpol_rep1_native.fastq.gz
2026-07-01 09:58:27,688 Reduced DataFrame memory usage from 0.1126556396484375Mb to 0.04232215881347656Mb
2026-07-01 09:58:27,696 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:27,701 Nanoget: Decompressing gzipped fastq Ngru_rep1_ivt.fastq.gz
2026-07-01 09:58:27,893 Reduced DataFrame memory usage from 0.0618133544921875Mb to 0.023256301879882812Mb
2026-07-01 09:58:27,894 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:27,900 Nanoget: Decompressing gzipped fastq Ngru_rep1_native.fastq.gz
2026-07-01 09:58:28,187 Reduced DataFrame memory usage from 0.03631591796875Mb to 0.01369476318359375Mb
2026-07-01 09:58:28,208 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:28,212 Nanoget: Decompressing gzipped fastq Nvec_rep1_ivt.fastq.gz
2026-07-01 09:58:28,417 Reduced DataFrame memory usage from 0.023773193359375Mb to 0.008991241455078125Mb
2026-07-01 09:58:28,423 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:28,431 Nanoget: Decompressing gzipped fastq Nvec_rep1_native.fastq.gz
2026-07-01 09:58:28,543 Reduced DataFrame memory usage from 0.0790557861328125Mb to 0.029722213745117188Mb
2026-07-01 09:58:28,558 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:28,563 Nanoget: Decompressing gzipped fastq Scer_rep1_ivt.fastq.gz
2026-07-01 09:58:28,953 Reduced DataFrame memory usage from 0.103118896484375Mb to 0.038745880126953125Mb
2026-07-01 09:58:28,954 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:28,959 Nanoget: Decompressing gzipped fastq Scer_rep1_native.fastq.gz
2026-07-01 09:58:29,156 Reduced DataFrame memory usage from 0.0741729736328125Mb to 0.027891159057617188Mb
2026-07-01 09:58:29,163 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:29,163 Nanoget: Decompressing gzipped fastq Scin_rep1_ivt.fastq.gz
2026-07-01 09:58:29,400 Reduced DataFrame memory usage from 0.1125030517578125Mb to 0.04226493835449219Mb
2026-07-01 09:58:29,414 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:29,420 Nanoget: Decompressing gzipped fastq Scin_rep1_native.fastq.gz
2026-07-01 09:58:29,559 Reduced DataFrame memory usage from 0.0814666748046875Mb to 0.030626296997070312Mb
2026-07-01 09:58:29,573 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:29,578 Nanoget: Decompressing gzipped fastq Skew_rep1_ivt.fastq.gz
2026-07-01 09:58:29,612 Reduced DataFrame memory usage from 0.040496826171875Mb to 0.015262603759765625Mb
2026-07-01 09:58:29,630 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:29,630 Nanoget: Decompressing gzipped fastq Skew_rep1_native.fastq.gz
2026-07-01 09:58:29,642 Reduced DataFrame memory usage from 0.1204376220703125Mb to 0.04524040222167969Mb
2026-07-01 09:58:29,655 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:29,664 Nanoget: Decompressing gzipped fastq Smic_rep1_ivt.fastq.gz
2026-07-01 09:58:29,917 Reduced DataFrame memory usage from 0.0223541259765625Mb to 0.008459091186523438Mb
2026-07-01 09:58:29,931 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:29,931 Nanoget: Decompressing gzipped fastq Smic_rep1_native.fastq.gz
2026-07-01 09:58:30,182 Reduced DataFrame memory usage from 0.0525970458984375Mb to 0.019800186157226562Mb
2026-07-01 09:58:30,203 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:30,208 Nanoget: Decompressing gzipped fastq Spun_rep1_ivt.fastq.gz
2026-07-01 09:58:30,275 Reduced DataFrame memory usage from 0.05657958984375Mb to 0.02129364013671875Mb
2026-07-01 09:58:30,275 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:30,279 Nanoget: Decompressing gzipped fastq Spun_rep1_native.fastq.gz
2026-07-01 09:58:30,337 Reduced DataFrame memory usage from 0.0391998291015625Mb to 0.014776229858398438Mb
2026-07-01 09:58:30,366 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:30,371 Nanoget: Decompressing gzipped fastq Tthe_rep1_ivt.fastq.gz
2026-07-01 09:58:30,436 Reduced DataFrame memory usage from 0.0645294189453125Mb to 0.024274826049804688Mb
2026-07-01 09:58:30,437 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-01 09:58:30,437 Nanoget: Decompressing gzipped fastq Tthe_rep1_native.fastq.gz
2026-07-01 09:58:30,535 Reduced DataFrame memory usage from 0.0369415283203125Mb to 0.013929367065429688Mb
2026-07-01 09:58:30,759 Reduced DataFrame memory usage from 0.0464019775390625Mb to 0.017477035522460938Mb
2026-07-01 09:58:30,805 Reduced DataFrame memory usage from 0.0400390625Mb to 0.0150909423828125Mb
2026-07-01 09:58:30,851 Reduced DataFrame memory usage from 0.1225738525390625Mb to 0.04604148864746094Mb
2026-07-01 09:58:30,877 Nanoget: Gathered all metrics of 172581 reads
2026-07-01 09:58:30,878 Using sequenced read lengths for plotting.
2026-07-01 09:58:31,463 NanoComp: Creating barplots for number of reads and total throughput.
2026-07-01 09:58:32,449 Saved ../report/nanocomp_fastq/NanoComp_number_of_reads  as png (or png for --legacy)
2026-07-01 09:58:33,329 Saved ../report/nanocomp_fastq/NanoComp_total_throughput  as png (or png for --legacy)
2026-07-01 09:58:34,433 Saved ../report/nanocomp_fastq/NanoComp_N50  as png (or png for --legacy)
2026-07-01 09:58:34,441 NanoComp: Creating violin plot for lengths.
2026-07-01 09:58:37,518 Saved ../report/nanocomp_fastq/NanoComp_lengths_violin  as png (or png for --legacy)
2026-07-01 09:58:37,524 NanoComp: Creating violin plot for log length.
2026-07-01 09:58:40,722 Saved ../report/nanocomp_fastq/NanoComp_log_length_violin  as png (or png for --legacy)
2026-07-01 09:58:40,724 NanoComp: Creating violin plot for quals.
2026-07-01 09:58:43,662 Saved ../report/nanocomp_fastq/NanoComp_quals_violin  as png (or png for --legacy)
2026-07-01 09:58:44,910 Saved ../report/nanocomp_fastq/NanoComp_OverlayHistogram  as png (or png for --legacy)
2026-07-01 09:58:46,122 Saved ../report/nanocomp_fastq/NanoComp_OverlayHistogram_Normalized  as png (or png for --legacy)
2026-07-01 09:58:47,372 Saved ../report/nanocomp_fastq/NanoComp_OverlayLogHistogram  as png (or png for --legacy)
2026-07-01 09:58:48,763 Saved ../report/nanocomp_fastq/NanoComp_OverlayLogHistogram_Normalized  as png (or png for --legacy)
2026-07-01 09:58:48,766 Writing html report.
2026-07-01 09:58:48,872 Succesfully processed all input.