General summary: Acas_rep1_ivt.fastq.gz Acas_rep1_native.fastq.gz Atha_rep1_ivt.fastq.gz Atha_rep1_native.fastq.gz Bnat_rep1_ivt.fastq.gz Bnat_rep1_native.fastq.gz Cfra_rep1_ivt.fastq.gz Cfra_rep1_native.fastq.gz Cowc_rep1_ivt.fastq.gz Cowc_rep1_native.fastq.gz Ddis_rep1_ivt.fastq.gz Ddis_rep1_native.fastq.gz Gthe_rep1_ivt.fastq.gz Gthe_rep1_native.fastq.gz Hsap_rep1_ivt.fastq.gz Hsap_rep1_native.fastq.gz Itha_rep1_ivt.fastq.gz Itha_rep1_native.fastq.gz Mlei_rep1_ivt.fastq.gz Mlei_rep1_native.fastq.gz Mmus_rep1_ivt.fastq.gz Mmus_rep1_native.fastq.gz Mpol_rep1_ivt.fastq.gz Mpol_rep1_native.fastq.gz Ngru_rep1_ivt.fastq.gz Ngru_rep1_native.fastq.gz Nvec_rep1_ivt.fastq.gz Nvec_rep1_native.fastq.gz Scer_rep1_ivt.fastq.gz Scer_rep1_native.fastq.gz Scin_rep1_ivt.fastq.gz Scin_rep1_native.fastq.gz Skew_rep1_ivt.fastq.gz Skew_rep1_native.fastq.gz Smic_rep1_ivt.fastq.gz Smic_rep1_native.fastq.gz Spun_rep1_ivt.fastq.gz Spun_rep1_native.fastq.gz Tthe_rep1_ivt.fastq.gz Tthe_rep1_native.fastq.gz Mean read length: 285.3 447.6 289.6 378.2 239.1 276.9 279.0 331.3 481.0 922.6 265.4 708.2 254.0 305.3 264.5 567.3 292.9 320.8 376.5 543.5 243.5 669.4 308.1 593.0 410.1 536.5 235.0 258.3 226.9 209.3 373.1 619.7 305.6 384.5 333.7 345.6 294.3 617.2 304.2 126.1 Mean read quality: 13.8 10.3 10.1 11.2 13.5 9.9 13.5 13.0 14.0 14.6 13.9 15.7 13.4 9.8 12.6 11.2 10.0 10.3 11.4 10.8 12.7 12.3 13.1 11.4 13.6 12.9 12.5 10.3 13.8 10.4 10.1 11.0 12.4 11.7 12.4 11.9 11.1 12.4 11.0 7.6 Median read length: 246.0 156.0 266.0 145.0 213.0 140.0 248.0 173.0 366.0 785.0 218.0 166.0 205.0 155.0 262.0 189.0 200.0 150.0 322.0 188.0 240.0 244.0 259.0 182.0 309.0 169.0 227.0 124.0 204.0 97.0 312.0 202.0 274.0 191.0 286.0 188.0 237.0 210.0 281.0 89.0 Median read quality: 14.4 10.7 9.5 10.9 14.3 9.6 14.0 13.4 14.4 14.7 14.6 16.4 13.8 9.5 13.1 11.6 9.6 9.8 11.4 11.0 13.1 12.5 13.5 11.8 14.2 13.4 13.1 10.5 14.4 9.4 10.1 11.5 12.6 11.8 12.5 12.0 11.1 13.2 10.8 7.4 Number of reads: 292,599.0 227,639.0 167,702.0 166,842.0 95,370.0 98,964.0 115,088.0 200,850.0 98,740.0 300,960.0 324,084.0 492,285.0 290,842.0 119,750.0 279,549.0 205,231.0 106,112.0 129,726.0 217,994.0 224,713.0 307,583.0 252,755.0 184,933.0 135,101.0 313,384.0 429,918.0 82,285.0 339,340.0 224,410.0 253,230.0 120,422.0 202,376.0 174,898.0 77,797.0 185,574.0 127,277.0 116,890.0 110,326.0 137,647.0 355,633.0 Read length N50: 302.0 1,639.0 319.0 1,100.0 276.0 506.0 319.0 659.0 669.0 1,788.0 286.0 2,039.0 300.0 781.0 294.0 1,635.0 387.0 808.0 404.0 1,844.0 283.0 1,540.0 364.0 1,562.0 544.0 1,355.0 282.0 591.0 236.0 326.0 442.0 1,839.0 350.0 725.0 403.0 597.0 362.0 1,781.0 338.0 130.0 STDEV read length: 366.1 997.5 234.4 570.1 895.4 1,052.4 571.1 738.1 389.8 809.5 324.3 1,232.7 1,122.5 833.7 985.7 940.6 926.0 519.9 385.9 1,066.7 561.2 853.9 932.4 981.3 615.1 797.8 567.0 454.3 437.6 452.7 515.9 1,058.2 532.4 1,067.5 601.6 643.1 503.9 1,094.7 294.1 274.1 Total bases: 83,485,721.0 101,885,680.0 48,572,378.0 63,103,712.0 22,807,587.0 27,399,885.0 32,105,085.0 66,550,615.0 47,493,551.0 277,654,213.0 86,012,602.0 348,629,233.0 73,874,482.0 36,560,520.0 73,934,944.0 116,427,709.0 31,076,488.0 41,611,052.0 82,080,485.0 122,128,455.0 74,904,100.0 169,184,698.0 56,974,289.0 80,111,732.0 128,526,210.0 230,642,386.0 19,333,579.0 87,665,018.0 50,907,589.0 53,005,307.0 44,931,456.0 125,414,050.0 53,454,522.0 29,909,651.0 61,928,617.0 43,990,764.0 34,396,441.0 68,090,295.0 41,874,840.0 44,854,454.0 Number, percentage and megabases of reads above quality cutoffs >Q5: 288934 (98.7%) 83.1Mb 218111 (95.8%) 100.8Mb 163829 (97.7%) 48.2Mb 163539 (98.0%) 62.8Mb 93369 (97.9%) 22.4Mb 91343 (92.3%) 26.6Mb 112509 (97.8%) 31.9Mb 197407 (98.3%) 66.3Mb 97753 (99.0%) 47.4Mb 300323 (99.8%) 277.5Mb 319242 (98.5%) 85.3Mb 486208 (98.8%) 348.0Mb 287620 (98.9%) 71.4Mb 113127 (94.5%) 36.0Mb 274845 (98.3%) 73.3Mb 196502 (95.7%) 115.5Mb 93028 (87.7%) 29.8Mb 125065 (96.4%) 41.2Mb 213869 (98.1%) 81.7Mb 218510 (97.2%) 121.5Mb 301598 (98.1%) 74.3Mb 249545 (98.7%) 168.9Mb 182351 (98.6%) 56.6Mb 132881 (98.4%) 79.9Mb 307699 (98.2%) 128.0Mb 425882 (99.1%) 230.3Mb 80455 (97.8%) 19.0Mb 324715 (95.7%) 86.3Mb 219590 (97.9%) 50.5Mb 240992 (95.2%) 52.0Mb 118049 (98.0%) 44.7Mb 190675 (94.2%) 124.5Mb 171908 (98.3%) 53.2Mb 75182 (96.6%) 29.7Mb 183182 (98.7%) 61.6Mb 124713 (98.0%) 43.8Mb 113871 (97.4%) 34.0Mb 106861 (96.9%) 67.8Mb 133022 (96.6%) 41.4Mb 330403 (92.9%) 42.3Mb >Q7: 280414 (95.8%) 81.4Mb 184699 (81.1%) 96.0Mb 142671 (85.1%) 43.7Mb 149515 (89.6%) 60.9Mb 89927 (94.3%) 21.7Mb 74115 (74.9%) 24.3Mb 108573 (94.3%) 31.0Mb 190006 (94.6%) 65.5Mb 95208 (96.4%) 46.8Mb 298462 (99.2%) 276.2Mb 308398 (95.2%) 83.9Mb 463938 (94.2%) 344.6Mb 278907 (95.9%) 69.4Mb 96687 (80.7%) 33.7Mb 262159 (93.8%) 71.0Mb 176551 (86.0%) 111.5Mb 75830 (71.5%) 27.1Mb 108271 (83.5%) 39.1Mb 202987 (93.1%) 79.9Mb 196218 (87.3%) 117.8Mb 290509 (94.4%) 72.4Mb 239272 (94.7%) 166.1Mb 175977 (95.2%) 55.1Mb 124268 (92.0%) 78.4Mb 295055 (94.2%) 125.4Mb 409777 (95.3%) 228.3Mb 77147 (93.8%) 18.5Mb 280840 (82.8%) 81.5Mb 213257 (95.0%) 49.7Mb 198261 (78.3%) 48.2Mb 108839 (90.4%) 42.7Mb 168128 (83.1%) 121.5Mb 164627 (94.1%) 51.5Mb 71343 (91.7%) 29.0Mb 175676 (94.7%) 60.2Mb 116690 (91.7%) 42.5Mb 103399 (88.5%) 32.1Mb 99960 (90.6%) 66.7Mb 122432 (88.9%) 39.5Mb 212051 (59.6%) 29.9Mb >Q10: 249803 (85.4%) 72.0Mb 128873 (56.6%) 79.3Mb 75053 (44.8%) 25.0Mb 105261 (63.1%) 51.4Mb 75566 (79.2%) 17.6Mb 46366 (46.9%) 16.2Mb 94005 (81.7%) 26.9Mb 167051 (83.2%) 61.7Mb 86376 (87.5%) 43.7Mb 286490 (95.2%) 263.9Mb 276354 (85.3%) 77.4Mb 422574 (85.8%) 322.4Mb 246613 (84.8%) 61.2Mb 53427 (44.6%) 24.4Mb 218290 (78.1%) 59.3Mb 135992 (66.3%) 91.5Mb 49764 (46.9%) 18.6Mb 61112 (47.1%) 28.7Mb 151503 (69.5%) 63.5Mb 135661 (60.4%) 103.2Mb 244090 (79.4%) 63.0Mb 204037 (80.7%) 147.3Mb 144373 (78.1%) 46.6Mb 93110 (68.9%) 66.6Mb 262143 (83.6%) 115.5Mb 359431 (83.6%) 219.3Mb 62983 (76.5%) 15.3Mb 185292 (54.6%) 65.1Mb 194185 (86.5%) 45.1Mb 114879 (45.4%) 39.6Mb 61338 (50.9%) 28.7Mb 122821 (60.7%) 107.2Mb 130785 (74.8%) 42.5Mb 57691 (74.2%) 23.2Mb 136296 (73.4%) 50.3Mb 92869 (73.0%) 34.0Mb 72617 (62.1%) 24.2Mb 82522 (74.8%) 60.7Mb 82130 (59.7%) 27.9Mb 39930 (11.2%) 8.7Mb >Q12: 215012 (73.5%) 61.3Mb 76304 (33.5%) 47.8Mb 45084 (26.9%) 15.5Mb 65325 (39.2%) 39.9Mb 65854 (69.1%) 15.3Mb 30490 (30.8%) 10.0Mb 79155 (68.8%) 22.8Mb 134777 (67.1%) 55.2Mb 73206 (74.1%) 37.4Mb 260741 (86.6%) 236.4Mb 243889 (75.3%) 68.1Mb 401704 (81.6%) 310.6Mb 210261 (72.3%) 52.6Mb 30811 (25.7%) 16.0Mb 169486 (60.6%) 45.9Mb 93350 (45.5%) 59.7Mb 35688 (33.6%) 13.3Mb 38915 (30.0%) 21.1Mb 88466 (40.6%) 38.5Mb 90707 (40.4%) 82.3Mb 190796 (62.0%) 49.2Mb 149505 (59.2%) 114.5Mb 117327 (63.4%) 37.5Mb 63863 (47.3%) 46.2Mb 219825 (70.1%) 97.6Mb 300527 (69.9%) 197.6Mb 50219 (61.0%) 12.1Mb 111808 (32.9%) 42.8Mb 173093 (77.1%) 40.4Mb 90921 (35.9%) 36.0Mb 25068 (20.8%) 13.9Mb 93311 (46.1%) 90.2Mb 99347 (56.8%) 32.3Mb 37017 (47.6%) 12.7Mb 102994 (55.5%) 38.5Mb 64222 (50.5%) 20.9Mb 47486 (40.6%) 15.5Mb 68181 (61.8%) 53.8Mb 51720 (37.6%) 18.1Mb 13366 (3.8%) 4.6Mb >Q15: 121481 (41.5%) 37.1Mb 10127 (4.4%) 3.4Mb 16303 (9.7%) 5.6Mb 24230 (14.5%) 19.7Mb 39121 (41.0%) 9.7Mb 9773 (9.9%) 2.9Mb 44496 (38.7%) 13.7Mb 54267 (27.0%) 28.4Mb 42204 (42.7%) 20.6Mb 130879 (43.5%) 110.0Mb 144022 (44.4%) 41.0Mb 329515 (66.9%) 253.7Mb 92775 (31.9%) 28.0Mb 6740 (5.6%) 4.4Mb 75762 (27.1%) 21.7Mb 25194 (12.3%) 7.1Mb 15729 (14.8%) 5.6Mb 14862 (11.5%) 7.5Mb 26413 (12.1%) 10.0Mb 13679 (6.1%) 7.4Mb 83583 (27.2%) 22.0Mb 31791 (12.6%) 19.4Mb 62048 (33.6%) 20.9Mb 14074 (10.4%) 7.7Mb 127778 (40.8%) 57.6Mb 98402 (22.9%) 87.0Mb 21524 (26.2%) 5.6Mb 16992 (5.0%) 4.0Mb 92186 (41.1%) 24.0Mb 42862 (16.9%) 25.5Mb 3989 (3.3%) 1.8Mb 27653 (13.7%) 26.0Mb 42280 (24.2%) 14.3Mb 11643 (15.0%) 4.5Mb 47407 (25.5%) 17.7Mb 23798 (18.7%) 7.9Mb 15312 (13.1%) 4.7Mb 28549 (25.9%) 22.3Mb 17817 (12.9%) 5.8Mb 2760 (0.8%) 0.6Mb Top 5 highest mean basecall quality scores and their read lengths 1: 25.3 (225) 22.3 (94) 28.6 (447) 25.1 (122) 24.4 (767) 27.2 (177) 26.0 (155) 25.5 (512) 25.6 (1711) 26.8 (638) 25.8 (534) 36.5 (131) 23.9 (499) 22.5 (1797) 25.2 (537) 25.9 (75) 26.7 (157) 24.7 (406) 25.0 (126) 25.4 (1185) 27.7 (117) 24.7 (1780) 26.9 (124) 24.9 (1241) 28.5 (149) 27.2 (150) 23.8 (87) 26.6 (156) 26.3 (536) 29.9 (682) 22.3 (270) 24.5 (92) 22.8 (629) 26.4 (265) 27.3 (198) 25.5 (162) 23.8 (715) 26.1 (683) 24.5 (157) 33.2 (121) 2: 25.1 (290) 21.8 (708) 24.1 (644) 24.6 (123) 24.0 (173) 26.6 (116) 24.8 (164) 25.2 (875) 24.8 (770) 26.3 (1539) 24.6 (713) 36.3 (124) 23.8 (422) 22.5 (159) 24.2 (576) 25.4 (500) 26.5 (157) 24.3 (3620) 24.0 (133) 23.1 (321) 27.1 (69) 24.5 (1251) 24.4 (310) 24.3 (846) 26.9 (185) 25.8 (141) 22.4 (246) 25.6 (270) 26.2 (807) 26.9 (1727) 22.2 (612) 23.8 (117) 22.5 (610) 24.2 (155) 26.4 (831) 25.5 (657) 23.1 (521) 25.1 (629) 24.1 (64) 29.8 (121) 3: 24.5 (223) 21.5 (296) 23.8 (78) 24.0 (121) 24.0 (360) 26.1 (161) 24.7 (126) 24.8 (732) 23.6 (1192) 25.8 (638) 24.5 (1132) 36.2 (94) 23.6 (392) 21.8 (94) 24.1 (619) 25.2 (785) 26.0 (166) 23.9 (537) 23.8 (340) 23.1 (298) 26.0 (94) 24.2 (3095) 24.4 (290) 23.9 (940) 25.3 (100) 25.6 (924) 22.0 (323) 23.4 (329) 25.8 (1087) 26.7 (551) 22.0 (851) 23.7 (94) 22.5 (573) 24.2 (515) 25.6 (168) 25.3 (162) 22.6 (239) 24.7 (1539) 23.5 (229) 29.1 (122) 4: 24.5 (537) 21.4 (1250) 23.1 (411) 23.6 (122) 23.8 (728) 24.9 (122) 24.5 (157) 24.6 (738) 23.5 (468) 25.7 (931) 24.4 (661) 36.0 (124) 23.5 (492) 21.7 (81) 23.6 (52) 25.1 (871) 25.9 (157) 23.2 (165) 23.7 (150) 22.8 (97) 26.0 (122) 24.2 (130) 24.4 (367) 23.9 (1053) 24.3 (154) 24.5 (1116) 21.9 (301) 23.3 (214) 25.7 (1662) 26.6 (1562) 21.9 (159) 23.5 (81) 22.5 (486) 24.1 (294) 25.5 (706) 25.2 (56) 22.6 (159) 24.5 (549) 23.2 (188) 28.5 (121) 5: 24.4 (112) 21.2 (157) 22.8 (874) 23.5 (491) 23.8 (1370) 24.8 (233) 24.3 (188) 24.3 (629) 23.4 (291) 25.6 (783) 24.4 (529) 36.0 (124) 23.5 (482) 21.6 (128) 23.6 (214) 25.0 (589) 25.6 (131) 23.1 (606) 23.4 (225) 22.6 (318) 25.9 (366) 24.2 (832) 23.9 (116) 23.8 (1182) 24.1 (633) 24.4 (254) 21.9 (319) 22.8 (47) 25.7 (2288) 26.3 (1506) 21.5 (405) 23.5 (120) 22.4 (474) 24.1 (415) 25.5 (194) 25.1 (592) 22.5 (310) 24.4 (2206) 23.2 (375) 28.1 (120) Top 5 longest reads and their mean basecall quality score 1: 117422 (6.0) 196401 (7.5) 40009 (7.8) 43942 (7.8) 202701 (3.3) 169481 (7.8) 87963 (6.4) 253137 (7.5) 41322 (7.9) 72623 (7.8) 61147 (7.9) 212321 (7.8) 311846 (4.1) 146469 (6.8) 340972 (7.3) 110674 (7.6) 187090 (7.4) 46654 (7.8) 91305 (8.1) 304001 (6.1) 167855 (7.8) 61095 (7.7) 351654 (6.0) 156635 (7.9) 178589 (6.9) 54592 (7.9) 124933 (4.2) 116141 (7.2) 108375 (7.8) 29197 (8.2) 89096 (7.9) 226803 (7.6) 180146 (6.5) 231195 (8.1) 212765 (7.9) 83545 (7.7) 116058 (4.1) 206518 (7.9) 52805 (6.3) 118004 (7.3) 2: 61899 (4.4) 170197 (7.7) 17671 (7.5) 32231 (7.9) 100295 (5.1) 125435 (7.9) 84402 (7.4) 39647 (7.7) 11747 (7.8) 69766 (6.8) 58872 (7.7) 105406 (8.0) 264385 (4.3) 107329 (7.1) 230788 (7.8) 86975 (7.8) 129402 (5.6) 38077 (8.0) 55976 (7.5) 84537 (6.1) 122314 (6.5) 59205 (7.4) 127049 (7.7) 110387 (7.4) 175990 (6.7) 32691 (7.2) 73165 (3.1) 63898 (7.7) 104998 (7.9) 28570 (8.0) 82533 (5.5) 87618 (7.6) 74807 (7.8) 96959 (7.8) 55718 (3.5) 73130 (7.9) 86619 (5.0) 47482 (8.1) 29814 (7.2) 40239 (5.9) 3: 40089 (8.0) 131868 (7.6) 11953 (5.7) 30930 (7.2) 85936 (6.6) 114620 (7.9) 81392 (5.3) 32179 (7.4) 9450 (7.8) 66666 (6.0) 42132 (4.8) 76556 (8.1) 232631 (4.0) 86024 (7.5) 211418 (6.0) 74580 (5.3) 81447 (7.7) 28865 (7.8) 43760 (7.8) 40311 (6.3) 96110 (6.2) 39852 (6.6) 93033 (4.7) 78558 (8.0) 84275 (6.3) 28399 (7.3) 52567 (8.0) 39373 (7.9) 74999 (7.9) 22311 (8.6) 77050 (7.0) 75097 (6.7) 41831 (5.3) 62992 (5.6) 38437 (7.4) 60348 (7.4) 30783 (6.8) 46975 (7.8) 21426 (7.4) 27310 (7.7) 4: 36411 (7.8) 106320 (7.3) 10501 (7.1) 21351 (8.0) 64796 (8.2) 79646 (7.5) 78048 (6.1) 24437 (7.8) 6322 (5.2) 49854 (7.9) 35856 (5.2) 75697 (8.1) 152596 (3.6) 81816 (7.3) 138953 (4.4) 60297 (6.9) 55322 (7.4) 25115 (7.8) 37733 (7.3) 35423 (7.7) 72913 (6.1) 39285 (7.6) 31450 (6.5) 36588 (6.1) 64607 (7.7) 26800 (8.3) 26414 (5.1) 29950 (7.7) 56020 (7.7) 20116 (8.6) 28628 (3.9) 47031 (8.0) 21955 (7.8) 29910 (8.2) 34910 (5.3) 56941 (7.9) 21436 (7.8) 45087 (7.9) 19011 (8.0) 23618 (6.6) 5: 34829 (7.8) 95120 (7.7) 8582 (7.9) 20699 (8.0) 51889 (7.5) 76553 (7.9) 54978 (7.8) 6139 (4.5) 6236 (7.1) 48735 (7.8) 32970 (4.6) 75425 (8.1) 142885 (3.9) 51315 (7.8) 126937 (6.2) 56706 (6.8) 46118 (7.5) 18724 (8.5) 28233 (5.9) 33864 (7.6) 65696 (7.7) 38980 (5.8) 28826 (8.1) 34312 (7.1) 38669 (5.6) 20716 (8.0) 19608 (4.0) 16212 (8.2) 44172 (7.6) 13436 (7.6) 14897 (7.4) 45165 (7.4) 17416 (5.3) 20492 (7.6) 29253 (8.0) 36596 (7.7) 16406 (6.2) 27954 (8.2) 11407 (5.3) 22604 (7.7)