2026-07-02 08:27:39,504 NanoComp 1.18.0 started with arguments Namespace(threads=4, outdir='../report/nanocomp_fastq/', prefix='', verbose=False, raw=False, store=False, tsv_stats=False, no_static=False, readtype='1D', maxlength=None, minlength=None, barcoded=False, split_runs=False, format='png', names=None, colors=None, plot='violin', title=None, dpi=100, fasta=None, fastq=['Acas_rep1_ivt.fastq.gz', 'Acas_rep1_native.fastq.gz', 'Atha_rep1_ivt.fastq.gz', 'Atha_rep1_native.fastq.gz', 'Bnat_rep1_ivt.fastq.gz', 'Bnat_rep1_native.fastq.gz', 'Cfra_rep1_ivt.fastq.gz', 'Cfra_rep1_native.fastq.gz', 'Cowc_rep1_ivt.fastq.gz', 'Cowc_rep1_native.fastq.gz', 'Ddis_rep1_ivt.fastq.gz', 'Ddis_rep1_native.fastq.gz', 'Gthe_rep1_ivt.fastq.gz', 'Gthe_rep1_native.fastq.gz', 'Hsap_rep1_ivt.fastq.gz', 'Hsap_rep1_native.fastq.gz', 'Itha_rep1_ivt.fastq.gz', 'Itha_rep1_native.fastq.gz', 'Mlei_rep1_ivt.fastq.gz', 'Mlei_rep1_native.fastq.gz', 'Mmus_rep1_ivt.fastq.gz', 'Mmus_rep1_native.fastq.gz', 'Mpol_rep1_ivt.fastq.gz', 'Mpol_rep1_native.fastq.gz', 'Ngru_rep1_ivt.fastq.gz', 'Ngru_rep1_native.fastq.gz', 'Nvec_rep1_ivt.fastq.gz', 'Nvec_rep1_native.fastq.gz', 'Scer_rep1_ivt.fastq.gz', 'Scer_rep1_native.fastq.gz', 'Scin_rep1_ivt.fastq.gz', 'Scin_rep1_native.fastq.gz', 'Skew_rep1_ivt.fastq.gz', 'Skew_rep1_native.fastq.gz', 'Smic_rep1_ivt.fastq.gz', 'Smic_rep1_native.fastq.gz', 'Spun_rep1_ivt.fastq.gz', 'Spun_rep1_native.fastq.gz', 'Tthe_rep1_ivt.fastq.gz', 'Tthe_rep1_native.fastq.gz'], fastq_rich=None, summary=None, bam=None, ubam=None, cram=None, pickle=None, feather=None, path='../report/nanocomp_fastq/')
2026-07-02 08:27:39,504 Python version is: 3.9.23 | packaged by conda-forge | (main, Jun  4 2025, 17:57:12)  [GCC 13.3.0]
2026-07-02 08:27:39,526 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:27:39,527 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:27:39,527 Nanoget: Decompressing gzipped fastq Acas_rep1_ivt.fastq.gz
2026-07-02 08:27:39,527 Nanoget: Decompressing gzipped fastq Acas_rep1_native.fastq.gz
2026-07-02 08:27:39,527 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:27:39,529 Nanoget: Decompressing gzipped fastq Atha_rep1_ivt.fastq.gz
2026-07-02 08:27:39,529 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:27:39,530 Nanoget: Decompressing gzipped fastq Atha_rep1_native.fastq.gz
2026-07-02 08:28:08,543 Reduced DataFrame memory usage from 2.559051513671875Mb to 1.2795867919921875Mb
2026-07-02 08:28:08,559 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:28:08,564 Nanoget: Decompressing gzipped fastq Bnat_rep1_ivt.fastq.gz
2026-07-02 08:28:14,217 Reduced DataFrame memory usage from 2.545928955078125Mb to 1.2730255126953125Mb
2026-07-02 08:28:14,226 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:28:14,232 Nanoget: Decompressing gzipped fastq Bnat_rep1_native.fastq.gz
2026-07-02 08:28:22,373 Reduced DataFrame memory usage from 1.455352783203125Mb to 0.7277374267578125Mb
2026-07-02 08:28:22,387 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:28:22,393 Nanoget: Decompressing gzipped fastq Cfra_rep1_ivt.fastq.gz
2026-07-02 08:28:27,383 Reduced DataFrame memory usage from 4.4648284912109375Mb to 2.2324752807617188Mb
2026-07-02 08:28:27,411 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:28:27,416 Nanoget: Decompressing gzipped fastq Cfra_rep1_native.fastq.gz
2026-07-02 08:28:30,588 Reduced DataFrame memory usage from 1.51019287109375Mb to 0.755157470703125Mb
2026-07-02 08:28:30,615 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:28:30,620 Nanoget: Decompressing gzipped fastq Cowc_rep1_ivt.fastq.gz
2026-07-02 08:28:34,458 Reduced DataFrame memory usage from 3.4736175537109375Mb to 1.7368698120117188Mb
2026-07-02 08:28:34,461 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:28:34,466 Nanoget: Decompressing gzipped fastq Cowc_rep1_native.fastq.gz
2026-07-02 08:28:40,923 Reduced DataFrame memory usage from 1.7562255859375Mb to 0.878173828125Mb
2026-07-02 08:28:40,943 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:28:40,951 Nanoget: Decompressing gzipped fastq Ddis_rep1_ivt.fastq.gz
2026-07-02 08:28:55,354 Reduced DataFrame memory usage from 1.50677490234375Mb to 0.753448486328125Mb
2026-07-02 08:28:55,376 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:28:55,383 Nanoget: Decompressing gzipped fastq Ddis_rep1_native.fastq.gz
2026-07-02 08:29:03,831 Reduced DataFrame memory usage from 3.064849853515625Mb to 1.5324859619140625Mb
2026-07-02 08:29:03,834 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:29:03,841 Nanoget: Decompressing gzipped fastq Gthe_rep1_ivt.fastq.gz
2026-07-02 08:29:31,196 Reduced DataFrame memory usage from 4.94525146484375Mb to 2.472686767578125Mb
2026-07-02 08:29:31,214 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:29:31,221 Nanoget: Decompressing gzipped fastq Gthe_rep1_native.fastq.gz
2026-07-02 08:29:47,791 Reduced DataFrame memory usage from 4.438018798828125Mb to 2.2190704345703125Mb
2026-07-02 08:29:47,801 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:29:47,811 Nanoget: Decompressing gzipped fastq Hsap_rep1_ivt.fastq.gz
2026-07-02 08:29:52,457 Reduced DataFrame memory usage from 1.827362060546875Mb to 0.9137420654296875Mb
2026-07-02 08:29:52,464 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:29:52,469 Nanoget: Decompressing gzipped fastq Hsap_rep1_native.fastq.gz
2026-07-02 08:30:31,933 Reduced DataFrame memory usage from 4.2657012939453125Mb to 2.1329116821289062Mb
2026-07-02 08:30:31,951 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:30:31,963 Nanoget: Decompressing gzipped fastq Itha_rep1_ivt.fastq.gz
2026-07-02 08:30:46,779 Reduced DataFrame memory usage from 4.5924072265625Mb to 2.2962646484375Mb
2026-07-02 08:30:46,808 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:30:46,814 Nanoget: Decompressing gzipped fastq Itha_rep1_native.fastq.gz
2026-07-02 08:30:50,619 Reduced DataFrame memory usage from 1.6192626953125Mb to 0.8096923828125Mb
2026-07-02 08:30:50,644 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:30:50,653 Nanoget: Decompressing gzipped fastq Mlei_rep1_ivt.fastq.gz
2026-07-02 08:30:53,930 Reduced DataFrame memory usage from 3.1316986083984375Mb to 1.5659103393554688Mb
2026-07-02 08:30:53,937 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:30:53,944 Nanoget: Decompressing gzipped fastq Mlei_rep1_native.fastq.gz
2026-07-02 08:31:10,866 Reduced DataFrame memory usage from 1.979583740234375Mb to 0.9898529052734375Mb
2026-07-02 08:31:10,888 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:31:10,893 Nanoget: Decompressing gzipped fastq Mmus_rep1_ivt.fastq.gz
2026-07-02 08:31:36,151 Reduced DataFrame memory usage from 3.326446533203125Mb to 1.6632843017578125Mb
2026-07-02 08:31:36,179 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:31:36,179 Nanoget: Decompressing gzipped fastq Mmus_rep1_native.fastq.gz
2026-07-02 08:31:39,756 Reduced DataFrame memory usage from 7.5117950439453125Mb to 3.7559585571289062Mb
2026-07-02 08:31:39,791 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:31:39,796 Nanoget: Decompressing gzipped fastq Mpol_rep1_ivt.fastq.gz
2026-07-02 08:31:56,784 Reduced DataFrame memory usage from 3.4289703369140625Mb to 1.7145462036132812Mb
2026-07-02 08:31:56,807 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:31:56,813 Nanoget: Decompressing gzipped fastq Mpol_rep1_native.fastq.gz
2026-07-02 08:31:56,830 Reduced DataFrame memory usage from 4.6934661865234375Mb to 2.3467941284179688Mb
2026-07-02 08:31:56,848 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:31:56,852 Nanoget: Decompressing gzipped fastq Ngru_rep1_ivt.fastq.gz
2026-07-02 08:32:12,957 Reduced DataFrame memory usage from 2.8219757080078125Mb to 1.4110488891601562Mb
2026-07-02 08:32:12,972 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:32:12,977 Nanoget: Decompressing gzipped fastq Ngru_rep1_native.fastq.gz
2026-07-02 08:32:38,627 Reduced DataFrame memory usage from 2.0615997314453125Mb to 1.0308609008789062Mb
2026-07-02 08:32:38,642 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:32:38,647 Nanoget: Decompressing gzipped fastq Nvec_rep1_ivt.fastq.gz
2026-07-02 08:32:50,461 Reduced DataFrame memory usage from 1.2556915283203125Mb to 0.6279067993164062Mb
2026-07-02 08:32:50,489 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:32:50,493 Nanoget: Decompressing gzipped fastq Nvec_rep1_native.fastq.gz
2026-07-02 08:33:02,398 Reduced DataFrame memory usage from 3.8568572998046875Mb to 1.9284896850585938Mb
2026-07-02 08:33:02,422 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:33:02,431 Nanoget: Decompressing gzipped fastq Scer_rep1_ivt.fastq.gz
2026-07-02 08:33:06,605 Reduced DataFrame memory usage from 4.781982421875Mb to 2.39105224609375Mb
2026-07-02 08:33:06,609 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:33:06,615 Nanoget: Decompressing gzipped fastq Scer_rep1_native.fastq.gz
2026-07-02 08:33:33,011 Reduced DataFrame memory usage from 3.424346923828125Mb to 1.7122344970703125Mb
2026-07-02 08:33:33,027 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:33:33,033 Nanoget: Decompressing gzipped fastq Scin_rep1_ivt.fastq.gz
2026-07-02 08:33:38,834 Reduced DataFrame memory usage from 3.864105224609375Mb to 1.4491157531738281Mb
2026-07-02 08:33:38,857 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:33:38,860 Nanoget: Decompressing gzipped fastq Scin_rep1_native.fastq.gz
2026-07-02 08:33:42,955 Reduced DataFrame memory usage from 5.17803955078125Mb to 2.589080810546875Mb
2026-07-02 08:33:42,983 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:33:42,986 Nanoget: Decompressing gzipped fastq Skew_rep1_ivt.fastq.gz
2026-07-02 08:33:58,573 Reduced DataFrame memory usage from 1.837615966796875Mb to 0.9188690185546875Mb
2026-07-02 08:33:58,596 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:33:58,600 Nanoget: Decompressing gzipped fastq Skew_rep1_native.fastq.gz
2026-07-02 08:34:08,594 Reduced DataFrame memory usage from 6.560150146484375Mb to 3.2801361083984375Mb
2026-07-02 08:34:08,621 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:34:08,632 Nanoget: Decompressing gzipped fastq Smic_rep1_ivt.fastq.gz
2026-07-02 08:34:14,015 Reduced DataFrame memory usage from 2.668853759765625Mb to 1.3344879150390625Mb
2026-07-02 08:34:14,037 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:34:14,037 Nanoget: Decompressing gzipped fastq Smic_rep1_native.fastq.gz
2026-07-02 08:34:15,183 Reduced DataFrame memory usage from 1.1872100830078125Mb to 0.5936660766601562Mb
2026-07-02 08:34:15,184 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:34:15,190 Nanoget: Decompressing gzipped fastq Spun_rep1_ivt.fastq.gz
2026-07-02 08:34:35,462 Reduced DataFrame memory usage from 1.783721923828125Mb to 0.8919219970703125Mb
2026-07-02 08:34:35,484 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:34:35,490 Nanoget: Decompressing gzipped fastq Spun_rep1_native.fastq.gz
2026-07-02 08:34:38,939 Reduced DataFrame memory usage from 1.9422149658203125Mb to 0.9711685180664062Mb
2026-07-02 08:34:38,940 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:34:38,946 Nanoget: Decompressing gzipped fastq Tthe_rep1_ivt.fastq.gz
2026-07-02 08:34:41,804 Reduced DataFrame memory usage from 3.088134765625Mb to 1.54412841796875Mb
2026-07-02 08:34:41,822 Nanoget: Starting to collect statistics from plain fastq file.
2026-07-02 08:34:41,828 Nanoget: Decompressing gzipped fastq Tthe_rep1_native.fastq.gz
2026-07-02 08:34:43,554 Reduced DataFrame memory usage from 2.831756591796875Mb to 1.4159393310546875Mb
2026-07-02 08:34:58,625 Reduced DataFrame memory usage from 2.1004486083984375Mb to 1.0502853393554688Mb
2026-07-02 08:35:01,479 Reduced DataFrame memory usage from 1.683563232421875Mb to 0.8418426513671875Mb
2026-07-02 08:35:03,016 Reduced DataFrame memory usage from 5.4266510009765625Mb to 2.7133865356445312Mb
2026-07-02 08:35:03,210 Nanoget: Gathered all metrics of 8286819 reads
2026-07-02 08:35:03,211 Using sequenced read lengths for plotting.
2026-07-02 08:35:27,203 NanoComp: Creating barplots for number of reads and total throughput.
2026-07-02 08:35:36,723 Saved ../report/nanocomp_fastq/NanoComp_number_of_reads  as png (or png for --legacy)
2026-07-02 08:35:38,646 Saved ../report/nanocomp_fastq/NanoComp_total_throughput  as png (or png for --legacy)
2026-07-02 08:35:40,004 Saved ../report/nanocomp_fastq/NanoComp_N50  as png (or png for --legacy)
2026-07-02 08:35:40,016 NanoComp: Creating violin plot for lengths.
2026-07-02 08:35:48,482 Saved ../report/nanocomp_fastq/NanoComp_lengths_violin  as png (or png for --legacy)
2026-07-02 08:35:48,514 NanoComp: Creating violin plot for log length.
2026-07-02 08:35:53,980 Saved ../report/nanocomp_fastq/NanoComp_log_length_violin  as png (or png for --legacy)
2026-07-02 08:35:53,982 NanoComp: Creating violin plot for quals.
2026-07-02 08:35:59,119 Saved ../report/nanocomp_fastq/NanoComp_quals_violin  as png (or png for --legacy)
2026-07-02 08:36:01,878 Saved ../report/nanocomp_fastq/NanoComp_OverlayHistogram  as png (or png for --legacy)
2026-07-02 08:36:04,680 Saved ../report/nanocomp_fastq/NanoComp_OverlayHistogram_Normalized  as png (or png for --legacy)
2026-07-02 08:36:07,590 Saved ../report/nanocomp_fastq/NanoComp_OverlayLogHistogram  as png (or png for --legacy)
2026-07-02 08:36:10,358 Saved ../report/nanocomp_fastq/NanoComp_OverlayLogHistogram_Normalized  as png (or png for --legacy)
2026-07-02 08:36:10,360 Writing html report.
2026-07-02 08:36:10,621 Succesfully processed all input.