FastQCFastQC Report
Mon 6 Apr 2020
fast5.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamefast5.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences430923
Sequences flagged as poor quality0
Sequence length1-54309
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGATC131553.0527495631470125No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTAAAGTTTACGCTAGATCCG39790.9233668195942198No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTTAAAGTTTACGCTAGATCC24410.5664585088287235No Hit
CCAGCTAGATCCGAAAGATTGGCTAAGTTGAACCAATTGTTGAGAATCGA23650.5488219473084519No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGTTAGATC17000.39450203400607536No Hit
AGATTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGATCCG8150.18912891630291256No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGATT8080.18750449616288756No Hit
AGATTGTGTTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGAT6780.157336693562423No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTTCTAAAGTTTACGCTAGAT6310.14642987262225501No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTTCTAAAGTTTACGCTAGATCC6200.1438772124022157No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGATA6160.1429489723222014No Hit
AGATTGTGTTTGTTAGTCGCATGGCTGTCTCTAAAGTTTACGCTAGATCC6020.1397001320421514No Hit
TCCAGCTAGATCCGAAAGATTGGCTAAGTTGAACCAATTGTTGAGAATCG5830.13529099166208347No Hit
AGATTGTGTTTGTTGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGATCC5580.12948949116199415No Hit
CAGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGAT5400.1253124108019298No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTAAAGTTTACGTTAGATCCG5320.12345593064190122No Hit
AGATTGTGTCTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGAT4910.1139414698217547No Hit
AGATTGTGTTTCTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGA4740.10999644948169396No Hit
AAGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGAT4720.10953232944168678No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAGTTTACGCTAGATCC4670.10837202934166892No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACAAGATCCG4530.1051231890616189No Hit
AGATTGTGTTTGTTAGTCGCTGGCTGTCTCTAAAGTTTACGCTAGATCCG4510.10465906902161176No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)