FastQCFastQC Report
Fri 20 Mar 2020
wt.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamewt.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences428120
Sequences flagged as poor quality0
Sequence length1-38943
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CT81921.9134822012519856No Hit
A37810.8831635989909371No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGATC27560.643744744464169No Hit
GT23310.5444735120994114No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTAAAGTTTACGCTAGATCCG18530.43282257311034295No Hit
C16240.37933289731850883No Hit
G12060.28169672054564143No Hit
CGT10970.25623656918620946No Hit
GCT10790.25203214052134915No Hit
CAGT6450.15065869382416144No Hit
CG5850.13664393160796037No Hit
GA5140.12005979631878913No Hit
T4840.11305241521068858No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGTTAGATC4720.11024946276744839No Hit
CAGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGAT4290.10020554984583761No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)