FastQCFastQC Report
Fri 20 Mar 2020
sl2_Viehweger_GR2019.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesl2_Viehweger_GR2019.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310957
Sequences flagged as poor quality0
Sequence length1-30551
%GC62

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGAUUGUGUUUGUUAGUCGCUAUGGCUGUCUCUAAAGUUUACGCUAGAUC25930.8338773528172706No Hit
CU22490.7232511247535832No Hit
AGAUUGUGUUUGUUAGUCGCUAUGGCUGUCUAAAGUUUACGCUAGAUCCG18510.5952591515868754No Hit
A11090.35664095035648014No Hit
GU7150.2299353286788849No Hit
C4870.15661329379946423No Hit
AGAUUGUGUUUGUUAGUCGCUAUGGCUGUCUCUAAAGUUUACGUUAGAUC4860.15629170592718608No Hit
G4350.13989072444099987No Hit
CGU3690.11866592487064127No Hit
CAGAUUGUGUUUGUUAGUCGCUAUGGCUGUCUCUAAAGUUUACGCUAGAU3620.11641480976469416No Hit
GCU3520.11319893104191255No Hit
AGAUUGUGUUUGUUAGUCGCUAUGGCUGUCUAAAGUUUACGUUAGAUCCG3500.11255575529735623No Hit
AGAUUGUGUUUGUUAGUCGCUAUGGCUGUCUUAAAGUUUACGCUAGAUCC3480.1119125795527999No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)