FastQCFastQC Report
Sun 15 Mar 2020
sl2.fq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filenamesl2.fq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310957
Sequences flagged as poor quality0
Sequence length1-30538
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGATC25950.8345205285618268No Hit
CT22480.7229295368813051No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTAAAGTTTACGCTAGATCCG18520.5955807394591536No Hit
A11110.35728412610103644No Hit
GT7170.2305785044234412No Hit
C4870.15661329379946423No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGTTAGATC4830.1553269423103516No Hit
G4330.13924754869644357No Hit
CGT3720.11963068848747575No Hit
CAGATTGTGTTTGTTAGTCGCTATGGCTGTCTCTAAAGTTTACGCTAGAT3610.116093221892416No Hit
GCT3550.11416369465874703No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTAAAGTTTACGTTAGATCCG3480.1119125795527999No Hit
AGATTGTGTTTGTTAGTCGCTATGGCTGTCTTAAAGTTTACGCTAGATCC3450.11094781593596542No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.8)