FastQCFastQC Report
Wed 31 Aug 2016
CD01_15469_GAGTCA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameCD01_15469_GAGTCA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences47273805
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACGAGTCAATCTCGTATG863450.18264872057580303TruSeq Adapter, Index 9 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA110250.037.5537382
CTCGTAT107100.037.2195643
ATCGGAA114750.036.139323
ACGAGTC111700.035.9044633
TCTCGTA111100.035.8399242
TCGTATG111100.035.80031644
AGATCGG115700.035.781781
CGTCAGA120900.033.71742217
GTCACGA120500.033.51944730
CGAGTCA120650.033.45982434
ACACGTC125300.032.70877514
CACGAGT124150.032.42793332
ATCTCGT123600.032.3223341
TCGGAAG131400.031.6772234
ACGTCAG130400.031.3622416
AGTCACG129950.031.0982129
CGGAAGA134250.030.8616355
CACGTCA133450.030.67842515
GTCAATC138100.028.88099537
CACACGT161850.025.48537613
Produced by FastQC (version 0.11.2)