FastQCFastQC Report
Tue 15 Sep 2015
INPUT_cycl_11138_CACAGT.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameINPUT_cycl_11138_CACAGT.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44961597
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG57350.034.2987861
GATCGGA72600.027.2628652
CTCGTAT75350.025.2508743
ATCGGAA79600.024.9993573
TCTCGTA76900.024.7133142
TATCTCG77450.024.39581140
ACACGTC81900.024.09084514
TCGGAAG83050.023.8544334
CGTCAGA83250.023.70018417
ACGTCAG86050.022.90343516
ATCTCGT83700.022.75809741
CACGTCA88550.022.30649215
CGGAAGA89350.022.2463845
TCGTATG85700.022.17361544
CGTATGC39150.021.42229744
GCACACG104150.018.98645612
TCCGATC26500.017.84617
CACACGT112000.017.79318413
CCGATCT29400.016.0855568
TTCCGAT34300.013.7878046
Produced by FastQC (version 0.11.2)