FastQCFastQC Report
Tue 15 Sep 2015
INPUT_SS_11139_TTGGCA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameINPUT_SS_11139_TTGGCA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52254093
Sequences flagged as poor quality0
Sequence length50
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACTTGGCAATCTCGTATG1115780.21352968465073158TruSeq Adapter, Index 3 (97% over 35bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACTTGGCAATCTCGTATGC587840.11249645075649864TruSeq Adapter, Index 3 (97% over 35bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG139150.039.195511
CGTATGC88950.032.7520444
GATCGGA199500.027.5233382
ATCGGAA209600.026.213213
TCTCGTA204100.025.97277342
CTCGTAT203850.025.91830343
CGTCAGA211100.025.76796717
ACACGTC212700.025.65686414
TCGGAAG214750.025.5941684
ATCTCGT211250.025.15616841
ACGTCAG216850.025.1049916
CACGTCA219350.024.89908415
TCGTATG212900.024.78644844
CGGAAGA222200.024.7261315
AATCTCG233950.022.98794240
GCACACG239000.022.9807912
CACACGT244400.022.4910313
GGCAATC258750.020.8441637
ACTTGGC299550.018.26210233
AGAGCAC309050.017.9712819
Produced by FastQC (version 0.11.2)