FastQCFastQC Report
Tue 15 Sep 2015
DIA_cycl_11136_CGTAGA.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDIA_cycl_11136_CGTAGA.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51345371
Sequences flagged as poor quality0
Sequence length50
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACCGTAGAATCTCGTATG1005610.19585212462482743TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG134300.037.6272661
GATCGGA166150.030.5856952
CCGTAGA166900.029.78461334
CTCGTAT165550.029.34988443
TCTCGTA167500.029.12638742
ATCGGAA175250.029.0543233
ACCGTAG175300.028.44522933
CGTAGAA176150.028.28299535
TCGGAAG180550.028.1887254
CGTCAGA178800.028.18366217
ACACGTC179000.028.09073314
CGTATGC61150.027.75628744
ATCTCGT177300.027.70255541
TCGTATG174300.027.6811644
GTCACCG180600.027.586130
CACCGTA181200.027.5554532
ACGTCAG186250.027.15079516
CACGTCA187700.026.90589515
CGGAAGA193150.026.475225
GCACACG209800.024.20796612
Produced by FastQC (version 0.11.2)