FastQCFastQC Report
Tue 15 Sep 2015
DIA_SS_11137_TCAGAG.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameDIA_SS_11137_TCAGAG.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48915581
Sequences flagged as poor quality0
Sequence length50
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATCGGAAGAGCACACGTCAGAACTCCAGTCACTCAGAGATCTCGTATG1262980.2581958497027767Illumina Multiplexing PCR Primer 2.01 (97% over 34bp)
GATCGGAAGAGCACACGTCAGAACTCCAGTCACTCAGAGATCTCGTATGC607310.12415471462968006TruSeq Adapter, Index 3 (97% over 34bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGATCGG154050.038.5367431
CGTATGC90600.032.08489644
GATCGGA214650.027.80692
ATCGGAA220000.027.0960623
CTCGTAT216600.026.95155543
TCTCGTA216900.026.80272542
ACACGTC223150.026.30831514
TCGGAAG228150.026.0890334
CGTCAGA226200.025.95358517
ATCTCGT224300.025.92826841
TCGTATG226050.025.79712944
CACGTCA230700.025.6284915
ACGTCAG233250.025.33887916
CGGAAGA237050.025.1373835
GCACACG254400.023.29280712
CACACGT256550.023.02044113
GATCTCG295550.019.88597340
GTCACTC297950.019.68887930
AGAGCAC320250.018.805619
GAGATCT326700.018.13801638
Produced by FastQC (version 0.11.2)