FastQCFastQC Report
Wed 10 Jun 2015
10346_ACAGTG_L002_R2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
Filename10346_ACAGTG_L002_R2_001.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences84996559
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2211340.2601681792788812Illumina Single End PCR Primer 1 (100% over 50bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTCG262750.042.18286510
GTCGCCG256800.042.1639344
GCGTCGT263300.042.0038411
CGTCGTG264850.041.70866412
GATCGGA273600.041.0887831
GGTCGCC264800.040.6904743
TGGTCGC264950.040.6428942
GAGCGTC278050.039.9487049
GTCGTGT278300.039.85898213
GTGGTCG272650.039.73468441
ATCGGAA285650.039.2707632
CGTGTAG283100.038.77519215
TCGTGTA283800.038.74811614
TCGGAAG292900.038.268683
TCGGTGG287950.037.90469738
AGAGCGT296050.037.839228
TCTCGGT289300.037.4973336
CTCGGTG293950.036.85214237
CGGAAGA304600.036.690454
CGGTGGT300550.036.14790739
Produced by FastQC (version 0.11.2)