FastQCFastQC Report
Tue 27 Sep 2016
WNN_A_P4_12834_TAAGGCGA_read2_50k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN_A_P4_12834_TAAGGCGA_read2_50k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71288472
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC3436160.4820078062551264No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG3103310.43531722772792775No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA183750.026.14348444
GATTTCG196950.025.0171841
ATTTCGT197800.024.82111742
TTTCGTC197900.024.63019643
GTAGGAC330650.023.722973
GTCCTAC351750.023.5311741
TATGGCG280300.021.62535516
TAGGACC222250.020.6439784
CGGAAAA212650.020.36378739
TCACGTT228100.020.36153827
ACGGAAA212200.020.26172638
AATCACG213350.020.15109434
ACGTTTT242100.020.03923829
CGAGAAA235850.019.99733721
TCACGGA212000.019.93772136
GCGAGAA233200.019.92948220
CACGTTT242300.019.84972428
ATCACGG211300.019.80488435
CGTTTTT246150.019.47716530
ATGGCGA330100.019.12884717
Produced by FastQC (version 0.11.2)