FastQCFastQC Report
Tue 27 Sep 2016
WNN_A_P4_12834_TAAGGCGA_read1_50k_nuclei.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameWNN_A_P4_12834_TAAGGCGA_read1_50k_nuclei.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71288472
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACCCTAACC3590190.5036143852262677No Hit
GGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGGGTTAGG3119510.4375896849072596No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGTCA174600.025.4316444
ATTTCGT184050.024.65215142
GATTTCG186500.024.42265741
TTTCGTC184600.024.23316443
GTAGGAC350250.023.9383013
GTCCTAC344200.023.810621
TATGGCG292650.021.48711416
CGGAAAA222400.021.0541939
GCGAGAA242350.020.630620
CGAGAAA247650.020.52927821
AATCACG219650.020.41799234
ACGGAAA224600.020.34898638
TAGGACC228000.020.046144
ATCACGG221350.019.97295635
TCACGGA224600.019.85927436
TCACGTT218700.019.72193327
TAGGACG177000.019.4598084
ACGTTTT235950.019.36194429
TGGCGAG331850.019.2675318
TGTAGGA435650.019.2506282
Produced by FastQC (version 0.11.2)